Separation and Size Assessment of dsDNA Fragments by Anion-Exchange Chromatography (AEX)

Application Note High-Resolution Separations of Single and Double-Stranded Nucleic Acids Using Strong Anion-Exchange Chromatography Abraham S. Finny, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation, United States Published on November 18, 2025 Abstract High-Resolution Separations of Single and Double-Stranded Nucleic Acids…

Application Note Size and Purity Assessment of Single-Guide RNAs by Anion-Exchange Chromatography (AEX) Hua Yang, Stephan M. Koza, Ying Qing Yu Waters Corporation Abstract Single-guide RNA (sgRNA) is a critical element in the CRISPR/Cas9 Technology for gene editing, the size…

Application Note Plasmid Isoform Separation and Quantification by Anion-Exchange Chromatography (AEX) Hua Yang, Stephan M. Koza, Weibin Chen Waters Corporation Abstract The purity and quality of plasmids play a critical role in the production of gene therapies and vaccines. Among…

Best Poster Award Participant (Poster #304) Strong Anion-Exchange HPLC for Size-Range and Topology-Resolved Analysis of Nucleic Acids Abraham S. Finny*, Fabrice G. Gritti, Balasubrahmanyam Addepalli, Matthew A. Lauber *[email protected] Waters Corporation, Milford, MA, USA Strong AEX resolves nucleic acids from…