Ion-Pairing Selection and Mixer Considerations in the Development of LC-MS Workflows

[ TECHNOLOGY BRIEF ] Application of Difluoroacetic Acid to Improve Optical and MS Performance in Peptide LC-UV/MS Jacob Kellett, Robert Birdsall, and Ying Qing Yu Waters Corporation, Milford, MA, USA Use of difluoroacetic acid as an ion-pairing agent improves optical…

Application Note Instrument Mixer Considerations to Improve Assay Reproducibility and Sensitivity David Dao, Robert E. Birdsall, Ying Qing Yu Waters Corporation This is an Application Brief and does not contain a detailed Experimental section. Abstract As part of a pharmaceutical…

Application Note Monitoring Impurities Using a High Throughput Focused Gradient With the Alliance™ iS Bio System Duanduan Han, Robert E. Birdsall, Karen Nyholm Waters Corporation Abstract The Alliance iS Bio HPLC System provides an efficient HPLC platform for routine monitoring…

Improving LC-MS Separations of Peptides with Difluoroacetic Acid Ion Pairing Jennifer Nguyen, Xiaoxiao Liu, and Matthew A. Lauber Waters Corporation, Milford, MA, USA INTRODUCTION Peptide mapping is an important and critical technique in the characterization of biopharmaceuticals. These separations are…