Troubleshooting Liquid Chromatographic Separations: Avoiding Common Issues through Informed Method Development Practices

Stability and Performance of Cyano Bonded Phase HPLC Columns for Reversed-Phase, Normal-Phase and HILIC Applications Richard A. Henry, Carmen T. Santasania, William H. Campbell and Wendy Roe sigma-aldrich.com T410171 Fundamentals of Ascentis® ES Cyano R. A. Henry EAS 2010 2…

Grouping Modern HPLC Columns Into Chemical Classes for Improving Phase Selection and Method Development Richard A. Henry, Hillel K. Brandes, William H. Campbell, Carmen T. Santasania, and David S. Bell Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA www.sigma-aldrich.com T410088…

Basics of Chiral HPLC Definitions Principles Available CSPs Mobile phase types T408109 sigma-aldrich.com The Field of Stereochemistry 1. All isomers have same chemical formula but differ in the arrangement of certain chemical groups in space. 2. Many types of isomers…

HybridSPE™ - Precipitation Technology Bridging the Gap between Simplicity & Selectivity in Sample Prep Updated: Mar 13 ‘09 T408104 sigma-aldrich.com Agenda • Introduction • HybridSPE-PPT (How does it work?) • LC Column Phospholipid Accumulation • Comparative Studies • Phospholipid Removal…