Automated online multicolumn 2D-LC screening as a rapid and efficient tool (Heather Wang, MDCW 2024)

🎤 Presenter: Heather Wang, Zach Dunn, Andrew Singh, Rodell Barrientos, Imad Haidar, Erik Regalado (Merck, Rahway, USA)

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💡 Book in your calendar: 16th Multidimensional Chromatography Workshop (MDCW) 3 - 5. February 2025

Abstract

Two-dimensional liquid chromatography (2D-LC) has become a valuable tool for improving peak capacity and selectivity. Though powerful, standard 2D-LC instrumentation and software can often lead to tedious method development that is poorly suited for a fast-paced industrial environment. In this regard, the introduction of an automated online 2D-LC setup that could screen multiple columns in both dimensions without manual intervention will undeniably serve to streamline column/mobile phase selection and secure the viability of 2D-LC as a mainstay instrument for industrial applications. Herein, we introduce and investigate a multicolumn online 2D-LC approach that simplifies column screening and method development dramatically enabling us to combine multiple columns in both dimensions. This strategy in conjunction with diode array detection (DAD) in both dimensions and mass spectrometry (MS) acquisition in the second dimension serves to explore different columns and mobile phases as a framework for screening targeted compounds in multicomponent mixtures without having to perform chromatographic purification. Multiple achiral – achiral and achiral – chiral online 2D-LC-DAD-ESI-MS methods combining several stationary phase selectivity in an automated fashion are successfully applied to the separation and analysis of (bio)pharmaceuticals, where in many instances, traditional 1D-UHPLC fails or delivers sub-optimal results.