Strategies for acquisition and processing of N-term acetylated peptides on the timsTOF Ultra 2

Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF) The timsTOF Pro with diaPASEF enables deeper proteome coverage in a single 4D shotgun proteomics experiment, with highly reproducible qualitative and quantitative results – making it a near-ideal mass analyzer…

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…

Quantitative analysis of signaling pathways using TMT 11plex reagents and comprehensive phosphopeptide enrichment strategies Bhavin Patel, Leigh Foster, Jae Choi, Ryan Bomgarden, Sergei Snovida, John C. Rogers, Thermo Fisher Scientific, Rockford, IL, USA ABSTRACT Results Purpose: There is broad interest…

APPLICATION NOTE 65381 Sequential enrichment using metal oxide affinity chromatography (SMOAC) to enhance phosphoproteome coverage for quantitative proteomic analysis Authors Jae Choi, Ryan Bomgarden, Bhavin Patel, Leigh Foster, Sergei Snovida, John C. Rogers Thermo Fisher Scientific, Rockford, IL, USA Introduction…