Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF)

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…

High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and…

High throughput proteomics – Application of dia-PASEF and Evosep One for short gradients ASMS 2020, MP122 Stephanie Kaspar-Schoenefeld1, Markus Lubeck1, Thomas Kosinski1, Scarlet Koch1, Oliver Raether1, Gary Kruppa1 1Bruker Daltonik GmbH, 28359 Bremen, Germany Introduction Data-independent acquisition (DIA) promises reproducible…