Sequential enrichment using metal oxide affinity chromatography (SMOAC) to enhance phosphoproteome coverage for quantitative proteomic analysis

Sequential enrichment from Metal Oxide Affinity Chromatography (SMOAC), a phosphoproteomics strategy for the separation of multiply phosphorylated from monophosphorylated peptides. Jae Choi, Sergei I. Snovida, Ryan Bomgarden, John C. Rogers, Thermo Fisher Scientific, Rockford, IL, 61101, USA Purpose: We explored…

Quantitative analysis of signaling pathways using TMT 11plex reagents and comprehensive phosphopeptide enrichment strategies Bhavin Patel, Leigh Foster, Jae Choi, Ryan Bomgarden, Sergei Snovida, John C. Rogers, Thermo Fisher Scientific, Rockford, IL, USA ABSTRACT Results Purpose: There is broad interest…

Quantitative, comprehensive multi-pathway signaling analysis using an optimized phosphopeptide enrichment method combined with an internal standard triggered targeted MS assay Bhavin Patel1; Penny Jensen1; Aaron S. Gajadhar2; Sebastien Gallien3; Jae Choi1; Romain Huguet2; Graeme McAlister2; Derek Bailey2; Shannon Eliuk2; Markus…

Quantitative, comprehensive multi-pathway signaling analysis using an optimized phosphopeptide enrichment method combined with an internal standard triggered targeted MS assay Bhavin Patel1; Penny Jensen1; Aaron S. Gajadhar2; Sebastien Gallien3; Jae Choi1; Romain Huguet2; Graeme McAlister2; Derek Bailey2; Shannon Eliuk2; Markus…