Enhanced Sensitivity and Reproducibility for HLA-Class I & II Immunopeptidomics Utilizing dia-PASEF Workflow

Exploring the power of the timsTOF Ultra to investigate the HLA immunopeptidome with high sensitivity and accuracy, allowing for smaller sample sizes while maintaining a robust immunopeptidome Benoit FATOU1, Diego ASSIS1, Nicholas CHEUNG2, Darshit SHAH2, Robert SALZLER2, Matthew WILLETTS1 1…

High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…