Structural Analysis of Native N-Glycans Released from Proteins Using a Novel Mixed-Mode Column and a Hybrid Quadrupole-Orbitrap Mass Spectrometer

See your protein therapeutics in high resolution Bio LC Columns Contents 2 Introduction to monoclonal antibody (mAb) characterization 3 Affinity titer determination 4 Aggregate separation 5 Charge variant analysis 6 Oxidation variant analysis 11 Antibody drug conjugates (ADCs) analysis 12…

In M M G N-linked glycans are released from proteins by PNGase-F. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased…

O-linked glycans are released from proteins by reductive β-elimination. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased performance and ease…

APPLICATION NOTE 73063 Improved profiling of sialylated N-linked glycans by ion chromatography-Orbitrap mass spectrometry Authors Sachin Patil and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords HPAE-PAD, glycoprotein, sialylated glycans, Q Exactive, HPAE-MS, HRAM, CarboPac PA200 column Goal To describe…