Label-Free Analysis by HPLC with Charged Aerosol Detection of Glycans Separated by Charge, Size and Isomeric Structure

Dave Thomas and Ian Acworth Thermo Fisher Scientific, Chelmsford, MA Ap plica t ion Note 1 1 27 Label-Free Analysis by UHPLC with Charged Aerosol Detection of Glycans Separated by Charge, Size, and Isomeric Structure Key Words Glycan Quantitation, Glycan…

O-linked glycans are released from proteins by reductive β-elimination. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased performance and ease…

Charge Profiling of 2AB-labelled N-linked Glycans The Agilent 1260 Infinity Bio-inert Quaternary LC with Fluorescence Detection Application Note Biologics and Biosimilars Author Abstract Sonja Schneider Agilent Technologies, Inc. Waldbronn, Germany This Application Note describes the analysis of charged N-linked glycans…

APPLICATION NOTE 73063 Improved profiling of sialylated N-linked glycans by ion chromatography-Orbitrap mass spectrometry Authors Sachin Patil and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords HPAE-PAD, glycoprotein, sialylated glycans, Q Exactive, HPAE-MS, HRAM, CarboPac PA200 column Goal To describe…