Sequential enrichment from Metal Oxide Affinity Chromatography (SMOAC), a phosphoproteomics strategy for the separation of multiply phosphorylated from monophosphorylated peptides.

APPLICATION NOTE 65381 Sequential enrichment using metal oxide affinity chromatography (SMOAC) to enhance phosphoproteome coverage for quantitative proteomic analysis Authors Jae Choi, Ryan Bomgarden, Bhavin Patel, Leigh Foster, Sergei Snovida, John C. Rogers Thermo Fisher Scientific, Rockford, IL, USA Introduction…

Quantitative analysis of signaling pathways using TMT 11plex reagents and comprehensive phosphopeptide enrichment strategies Bhavin Patel, Leigh Foster, Jae Choi, Ryan Bomgarden, Sergei Snovida, John C. Rogers, Thermo Fisher Scientific, Rockford, IL, USA ABSTRACT Results Purpose: There is broad interest…

Turnkey, Multi-pathway Signaling Analysis Using a Synthetic Phosphopeptide Panel, Standardized Sample Preparation Kits and SureQuant Internal Standard Targeted Quantitation Aaron S. Gajadhar1; Bhavin Patel2; Penny Jensen2; Sebastien Gallien3,4; Romain Huguet1; Kay Opperman2; John C Rogers2; Andreas Huhmer1; Daniel Lopez-Ferrer1 1Thermo…

Quantitative, comprehensive multi-pathway signaling analysis using an optimized phosphopeptide enrichment method combined with an internal standard triggered targeted MS assay Bhavin Patel1; Penny Jensen1; Aaron S. Gajadhar2; Sebastien Gallien3; Jae Choi1; Romain Huguet2; Graeme McAlister2; Derek Bailey2; Shannon Eliuk2; Markus…