Improving LC-MS/MS Bioanalytical Quantitation of Oligonucleotides using New High Performance Tandem Quadrupole Mass Spectrometer

DEVELOPMENT OF A BIOANALYTICAL SPE LC-MS/MS ASSAY FOR OLIGONUCLEOTIDES Mary Lame, Kathryn Brennan, Michael Donegan, Thanai Paxton, and Ian Edwards Waters Corporation, 34 Maple Street, Milford, MA , USA INTRODUCTION RESULTS Over the past decade, there has been a dramatic…

T1530 ‐09‐54 Sensitive Bioanalysis of Antisense Oligonucleotides through Mitigation of Non‐specific Binding and Improved LC‐MS/MS Performance Suma Veeramachineni, Mark D Wrona Waters Corporation, Milford, MA CONTACT INFORMATION: [email protected]; [email protected] PURPOSE RESULT(S) Improving LC-MS/MS bioanalytical performance especially for ESI negative analytes…

Application Note Sensitive LC-MS/MS Bioanalytical Quantitation of Antisense Oligonucleotides Suma Veeramachineni, Mark D. Wrona Waters Corporation Abstract This application demonstrates the quantitative and qualitative capabilities of the Waters™ Xevo™ TQ Absolute Tandem Quadrupole Mass Spectrometer (MS/MS) and its suitability for…

Application Note Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology Kathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. Rainville Waters Corporation Abstract The demand for highly selective and sensitive LC-MS bioanalytical assays in support of research and development…