Quantitative Analysis of Large Phosphopeptide Datasets Using Proteome Discoverer 2.0

INTRODUCTION POSTER NOTE Proteome Discoverer software is a node-based workflow engine and study management platform for analysis of mass spectrometry-based proteomics datasets. The latest released version 2.1 fully supports isotopically-labeled quantitative workflows, such as TMTTM reporter ion-based quantification and SILAC…

Methods A F e d 2 p l Sample Preparation Before committing valuable sample, instrument performance was verified using a standard Pierce HeLa cell digest with Biognosys HRM kit spiked in 1:10 according to the manufacturer’s instructions. Next, two non-small…

values, or to be used as replicates to generate standard errors. Here we present a new workflow for untargeted label-free quantification using a new feature detection approach that provides the full suite of quantitative capabilities previously only available for isotopicallylabeled…

APPLICATION NOTE 65381 Sequential enrichment using metal oxide affinity chromatography (SMOAC) to enhance phosphoproteome coverage for quantitative proteomic analysis Authors Jae Choi, Ryan Bomgarden, Bhavin Patel, Leigh Foster, Sergei Snovida, John C. Rogers Thermo Fisher Scientific, Rockford, IL, USA Introduction…