Fast profiling of the N-glycan population in biotherapeutic antibodies by UHPLC-FLD with MS confirmation

APPLICATION NOTE 21651 Comprehensive protein glycosylation comparison of an innovator monoclonal antibody to a candidate biosimilar by HILIC UHPLC analysis Authors Silvia Millán, Anne Trappe, Amy Farrell, and Jonathan Bones Characterisation and Comparability Laboratory, NIBRT – The National Institute for…

APPLICATION NOTE 21683 An ultrafast, batch-to-batch comparison of monoclonal antibody glycosylation Authors Silvia Millán, Stefan Mittermayr, Amy Farrell, and Jonathan Bones Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing Research and Training, Dublin, Ireland Keywords NIBRT, Biopharmaceutical,…

APPLICATION NOTE 21764 Accurate and precise quantification of mAb-released N-glycans with an amide HILIC column Authors Xin Zhang, Xuefei Sun Thermo Fisher Scientific, Sunnyvale, CA, USA Application benefits • Proven intra- and inter-column reproducibility in retention time, backpressure, and…

See your protein therapeutics in high resolution Bio LC Columns Contents 2 Introduction to monoclonal antibody (mAb) characterization 3 Affinity titer determination 4 Aggregate separation 5 Charge variant analysis 6 Oxidation variant analysis 11 Antibody drug conjugates (ADCs) analysis 12…