<p><strong>Join us for a free two-day LECO Separation Science Workshop on September 23–24, 2026, at the </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/companies/219"><strong>C³AL Laboratory</strong></a><strong> at Cal State LA. Registration is free, and meals and refreshments will be provided throughout the workshop.&nbsp;</strong></p><h4><strong>Registration</strong></h4><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://forms.cloud.microsoft/pages/responsepage.aspx?id=iYeNbqIuykW7qZP4_jUEVbznDB40eK1PgjPzhHyb__9URTFTTFA5UEEzQ1A4MUtFV0o4TEJTNlZCOS4u&amp;route=shorturl"><strong>Register HERE</strong></a></p><p class="cke-user_style-important"><strong>Space is limited, register today!</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/LECO_LECO_Separation_Science_Workshop_registration_332543a18e.jpg" alt="LECO: LECO Separation Science Workshop - QR code for registration" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_LECO_LECO_Separation_Science_Workshop_registration_332543a18e.jpg 156w," sizes="100vw" width="437" height="437"></p></blockquote><h4><strong>Hand-on Experience with the LECO Complex Chemical Composition Analysis Lab C<sup>3</sup>AL</strong></h4><ul><li>Work directly with LECO instruments in the C<sup>3</sup>AL Laboratory</li><li>Learn complete analytical workflows from sample to answer</li><li>Get expert guidance from LECO application scientists</li><li>Develop methods and solve real-world challenges</li><li>Collaborate, ask questions, and build your skills</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Cal_State_LA_The_LECO_Pegasus_BTX_system_in_Cal_State_LA_s_LECO_Complex_Chemical_Compositional_Analysis_Lab_enhances_research_and_student_training_in_advanced_chemical_analysis_cover_b739e727ca.jpg" alt="Cal State LA: The LECO Pegasus BTX system in Cal State LA’s LECO Complex Chemical Compositional Analysis Lab enhances research and student training in advanced chemical analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Cal_State_LA_The_LECO_Pegasus_BTX_system_in_Cal_State_LA_s_LECO_Complex_Chemical_Compositional_Analysis_Lab_enhances_research_and_student_training_in_advanced_chemical_analysis_cover_b739e727ca.jpg 227w," sizes="100vw" width="1252" height="863"></p><p>The first day will focus on the <strong>fundamentals and theory</strong> of comprehensive two-dimensional gas chromatography (<strong>GC×GC</strong>), with an emphasis on understanding how GC×GC can enhance your research, solve complex analytical challenges, and support your laboratory or company goals.&nbsp;</p><p>The second day will be dedicated to <strong>hands-on activities</strong>, giving participants the opportunity to work directly with <strong>LECO instrumentation</strong>, learn <strong>practical analytical workflows</strong>, and <strong>analyze their own samples</strong> with guidance from LECO application scientists.&nbsp;</p><p>Whether you are new to GC×GC or looking to expand its use in your research or organization, this workshop offers a unique opportunity to learn, explore, and apply the technology in a practical setting. Space is limited, so register early!</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Cal_State_LA_LECO_QUADJET_G_Cx_GC_coupled_to_Pegasus_BT_4_D_TOM_MS_in_the_Complex_Chemical_Composition_Analysis_Lab_C_AL_077a14ae34/Cal-State-LA-LECO-QUADJET-GCxGC-coupled-to-Pegasus-BT-4D-TOM-MS-in-the-Complex-Chemical-Composition-Analysis-Lab-(CAL).jpg" alt="Cal State LA: LECO QUADJET GCxGC coupled to Pegasus BT 4D TOM MS in the Complex Chemical Composition Analysis Lab (C³AL)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Cal_State_LA_LECO_QUADJET_G_Cx_GC_coupled_to_Pegasus_BT_4_D_TOM_MS_in_the_Complex_Chemical_Composition_Analysis_Lab_C_AL_077a14ae34/thumbnail_Cal-State-LA-LECO-QUADJET-GCxGC-coupled-to-Pegasus-BT-4D-TOM-MS-in-the-Complex-Chemical-Composition-Analysis-Lab-(CAL).jpg 234w," sizes="100vw" width="1949" height="1299"></p>

LECO Separation Science Workshop

<p><strong>Gas chromatography (GC) is a powerful analytical technique used to separate, identify, and quantify components within a complex mixture. The ability to achieve accurate and reliable separation is critical for both qualitative and quantitative analysis across numerous fields, including pharmaceuticals, environmental science, food safety, and petrochemical industries.</strong></p><p>By providing high-resolution data, gas chromatography plays a pivotal role in ensuring product quality, monitoring environmental pollutants, and advancing scientific research. Understanding the fundamental principles of GC enhances the capacity to apply this technique effectively, enabling more precise measurements and contributing to innovations in various applications.</p><h3><strong>Process and Principles of GC Separation</strong></h3><p>At its core, GC separates compounds based on differences in volatility and interactions with a stationary phase as the mixture moves through a column carried by an inert gas (mobile phase). Each compound’s unique behavior when exposed to heat and the stationary phase results in distinct retention times, enabling precise identification and analysis.</p><p>The sample mixture is vaporized and injected into a flow of inert carrier gas, such as helium or nitrogen, which propels the sample through a column lined with stationary phase material. As the sample travels, components interact differently with the stationary phase based on their volatilities and affinities. Stronger interactions slow movement, while weaker interactions lead to faster traversal, causing separation according to boiling points and polarity.</p><p>Over time, compounds elute at different rates, producing distinct peaks in the chromatogram. Each peak represents a compound, with retention time serving as its identifier. The area under the peak correlates with the compound’s quantity, allowing for accurate quantification.</p><p>Discover the wide range of <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/gc"><strong>GC applications and methods</strong></a> used across industries for accurate compound analysis and separation.</p><h3><strong>Operational steps in Gas Chromatography</strong></h3><p>The working of GC involves injecting and vaporizing samples, separating them in the column based on their affinities, and detecting them as distinct peaks. These components are then analyzed for precise identification and quantification. Here are the key operational steps involved in performing a gas chromatography analysis:</p><h4><strong>Sample Injection</strong></h4><p>The gas chromatography process begins with sample injection, where a small, precise amount of the sample is introduced into the system through a syringe. The injection process must be carefully controlled to prevent overloading the column, ensuring accurate and efficient separation.</p><p>Key components, such as syringe injectors, autosamplers and injection ports, are tailored to meet specific analytical needs. Injection techniques, including split, splitless, and on-column injection, are used to control the introduction based on the sample's concentration, volatility, and desired sensitivity. Proper selection and configuration of the injection system are essential for optimal separation, minimizing sample loss, and enhancing analytical precision.</p><h4><strong>Vaporization</strong></h4><p>Upon entering the heated injection port, the sample is vaporized, converting it into a gas phase. This vaporized sample mixes with the carrier gas, typically an inert gas such as helium or nitrogen. The carrier gas plays a crucial role in transporting the vaporized sample through the column, promoting even distribution and preventing condensation or loss of the sample during transit.</p><h4><strong>Separation</strong></h4><p>As the vaporized sample travels through the GC column, it interacts with the stationary phase lining the interior of the column. Separation occurs because each component of the sample interacts differently with the stationary phase, depending on its volatility and polarity. Compounds with a stronger affinity for the stationary phase move more slowly, while those with weaker interactions pass through more quickly. This differential movement leads to the separation of the sample into distinct components, allowing for their individual analysis.</p><h4><strong>Detection</strong></h4><p>When the separated compounds exit the column, they pass through a detector that identifies and measures their presence. Commonly used detectors include flame ionization detectors (FID) and mass spectrometers (MS). As each compound reaches the detector, it generates a signal that is recorded as a peak on the chromatogram. Each peak corresponds to a specific compound, and the time it takes to reach the detector (retention time) serves as an identifier.</p><h4><strong>Data Analysis</strong></h4><p>The chromatogram produced by the detector is analyzed to identify and quantify the compounds in the sample. Retention times are compared to known standards to determine the identity of each compound, while the area under each peak correlates with the concentration.</p><p>Advanced techniques enhance this process by employing mathematical algorithms to handle large datasets, facilitating pattern recognition, simultaneous compound analysis, and the optimization of separation parameters for improved analytical accuracy and efficiency.</p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/gas-chromatography"><strong>Explore advanced gas chromatography techniques</strong></a><strong> </strong>to enhance sensitivity, improve resolution, and optimize your analytical processes.</p></blockquote><h3><strong>Conclusion</strong></h3><p>A GC system comprises four primary components: the <strong>injection port</strong>, where the sample is introduced and vaporized; the <strong>carrier gas</strong>, which transports the vaporized sample through the column oven; the <strong>gas chromatography column</strong>, where separation of components occurs based on their interactions with the stationary phase; and the <strong>detector</strong>, which identifies and quantifies the separated analytes as they exit the column. Together, these elements enable precise separation, detection, and analysis of volatile compounds in diverse analytical applications.</p>

Understanding the Essential Components of Gas Chromatography

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 31st August 2026? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies</strong>, <strong>Shimadzu, Thermo Fisher Scientific </strong>and presentation by<strong> MDCW / Liège University!</strong></p><h3><strong>1. Agilent Technologies: Extend the time window for the detection of low level anabolic‑androgenic steroids and their metabolites</strong></h3><p><strong>Steroid profiling, screening, and confirmatory analysis using the </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=10&amp;search=7010"><strong>Agilent&nbsp;7010 GC/MS mass spectrometer</strong></a></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/5991_9045_EN_Doping_Control_App_Note_1_13dde5f053.pdf"><strong>Full PDF for download</strong></a></li></ul><section><div><div><div><div><div><div><p>Doping laboratories want to have lower detection limits to be able to identify and quantitate illegal drugs for a longer time window between doping and urine sample collection. This Application Note describes how the doping control lab in Kreischa, Germany used the Agilent 7010 Triple Quadrupole GC/MS System (TQ) for enhanced targeted detection of anabolic‑androgenic steroids in urine samples. The 7010 TQ enabled higher sensitivity, longer maintenance-free intervals, and more stable operation than earlier models, and satisfied all measurement specifications required by the World Anti‑Doping Agency (WADA). The 7010 TQ GC/MS ion source produces more ions, and provides exceptional sensitivity for the detection of low intensity metabolites weeks after drug ingestion. However, this higher sensitivity may lead to saturation of endogenous components naturally present at higher concentrations. This method provides a sensitive and robust operation over a wide concentration range, permitting the detection of low-concentration exogenous species in the presence of high‑concentration endogenous species, which can vary with ethnicity.</p><h4><strong>Experimental</strong></h4><p>The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=10&amp;search=7010"><strong>Agilent 7010 GC/TQ system</strong></a> consists of an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=10&amp;search=7890"><strong>Agilent 7890 GC</strong></a> with multimode inlet and backflush, an<strong> Agilent 7993A</strong>, and the Agilent 7010 TQ mass spectrometer.</p><h4><strong>Conclusions&nbsp;</strong></h4><p>Based on the method optimization and thorough testing at the Kreischa lab, an Agilent 7010 GC/MS-based protocol was developed. This protocol fulfills the screening and confirmation requirements of steroid measurements in doping control labs. With this method, it is possible to detect traces of exogenous anabolic steroid metabolites while quantifying small concentrations of testosterone and epitestosterone typical in Asian populations. It permits the measurement of high‑abundance endogenous steroids such as androsterone and etiocholanolone in the same injection, while analyzing testosterone/epitestosterone and androsterone/etiocholanolone ratios without suppression.&nbsp;</p><p>The backflush capability of the system protects the analytical column, and consequently the mass spectrometer, by removing heavy matrix components. This allowed more than 6,000 samples to be analyzed on the same column without requiring ion source cleaning or filament change. It also demonstrated that the higher, 130&nbsp;eV electron energy did not sacrifice the filament lifetime. It performed reliable measurements of both trace (0.2&nbsp;pg on‑column) and high (10,000&nbsp;pg on‑column) concentration analytes in the same run.&nbsp;</p><p>The method validation revealed that the exceptional sensitivity offered by the 7010 GC/MS can be used to:&nbsp;</p><ul><li>Improve the trace detection ability of the method&nbsp;</li><li>Reduce the amount of sample&nbsp;injected&nbsp;</li><li>Extend maintenance-free intervals</li></ul><h3><strong>2. MDCW / Liège University: The Century Mix: updates and cool projects</strong></h3><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/DG_1_Dimandja_Stefanuto_34c4a66c3d.pdf"><strong>Full PDF for download</strong></a></li></ul><section><div><div><div><div><div><div><p>The presentation <strong>“The Century Mix: updates and cool projects”</strong> introduces the Century Mix, a standardized reference mixture developed for comprehensive two-dimensional gas chromatography (GC×GC). The mixture contains <strong>100 compounds representing a broad range of chemical classes</strong>, including alkanes, alcohols, ketones, esters, lactones, phthalates, and aromatic compounds. Stability was a key criterion in compound selection, and the mixture was developed through cross-laboratory collaboration for <strong>column characterization, method assessment, and quality control</strong>. Its structured chromatographic behavior also makes it useful for studying how different compound classes behave in GC×GC separations.</p><p>A major part of the work examined the <strong>factors influencing retention indices (RI) in GC×GC systems</strong>, which are important for reliable compound annotation in non-targeted analysis. An inter-laboratory study involved <strong>11 GC×GC systems</strong> using cryogenic, Peltier, and flow modulation, different detectors, and both normal and reversed column configurations. Principal component analysis showed clear grouping according to normal versus reversed orthogonality, confirming the strong influence of stationary-phase configuration. A subsequent design-of-experiments study evaluated <strong>140 combinations</strong> of temperature ramp rate, carrier-gas flow rate, modulation period, and secondary-oven temperature offset.</p><p>The results showed that the influence of experimental conditions is strongly <strong>compound dependent</strong>. Of the compounds evaluated in the statistical model, 33 showed RI variation that could be linked to the investigated parameters, whereas 43 did not follow the model well. Compounds such as quinoline, phthalates, lactones, terpenes, and some aromatic compounds showed particularly pronounced effects. For quinoline, for example, the model explained about <strong>93% of RI variability</strong>, with differences of approximately 75 RI units across the tested conditions, while 2-hexanone showed considerably weaker dependence. Overall, <strong>temperature ramp rate and flow rate emerged as particularly important parameters</strong>, although the magnitude of their effect depended on compound chemistry and column configuration.</p><p>The Century Mix is therefore being developed not only as a characterization mixture but also as a tool for <strong>system quality control and more reliable retention-index prediction</strong>. Repeated standardized QC measurements demonstrated very low RI variability and can be used to monitor system stability, experimental effects, and potential instrument drift. The broader goal is to better understand the fundamental chemistry governing GC×GC retention and ultimately improve method development, data processing, and identification of unknown compounds by combining high-quality separations, mass spectra, and predicted retention information.</p><h3><strong>3. Shimadzu: Determination of Free and Total Glycerol and Mono-, Di-, and Triglyceride Contents in Biodiesel According to EN 14105</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_05_sca_180_055_en_9b8677ed6a.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>User benefits</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=1&amp;search=2060"><strong>Nexis GC-2060</strong></a> offers reliable analysis of biodiesel samples regarding their free and total glycerol and mono-, di-, and triglyceride contents, using hydrogen as a cost-effective carrier gas&nbsp;</li><li>Hydrogen carrier gas can be used safely through built-in safety measures</li><li>The multi-mode injection unit (MMI) allows selection of the preferred injection mode – direct (simple OCI) or convenient splitless PTV mode – both delivering excellent instrument performance in line with the standard’s requirements</li></ul><p>Playing a vital part in countries’ efforts to lower greenhouse gas emissions, biofuels can be manufactured from various feedstocks such as vegetable oils, animal-derived fats, and recycled cooking oils. Biodiesel production predominantly uses rapeseed, soybean, and palm oils. Because feedstocks are so varied, ongoing quality monitoring is essential - since the raw material’s quality greatly influencesthe final biodiesel quality.&nbsp;</p><p>The EN 14105:2024 describes the determination of free and total glycerol contents and mono-, di-, and triglyceride contents in biodiesel by means of gas chromatography with flame ionization detection (GC-FID). Injection shall be performed via on-column injector or an equivalent device<sup>1</sup>.&nbsp;</p><p>This study shows the analysis of biodiesel originating from rapeseed, soybean, and palm oil using the Nexis GC-2060 gas chromatograph and its multi-mode injection unit (MMI).</p><h4><strong>Configuration and Analysis Conditions</strong></h4><p>Gas chromatograph <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=1&amp;search=2060"><strong>Nexis GC-2060</strong></a> with multi-mode injection unit (MMI), flame-ionization detector (FID) and autosampler <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=1&amp;search=AOC+-30i"><strong>AOC -30i</strong></a>.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>Nexis and AOC are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.. Nexis GC-2060 provides reliable analysis of biodiesel samples for glycerol and glyceride contents according to EN 14105:2024 across the commonly used feedstocks. It supports hydrogen as a cost-effective carrier gas, while the multi-mode injection unit (MMI) offers selection of the injection mode based on user preference: direct mode representing simple on-column (OCI) analysis or splitless programmed temperature vaporization (PTV) mode as a convenient alternative that delivers comparable performance.</p><h3><strong>4. Thermo Fisher Scientific: Streamlined static headspace GC–MS approach for detection of impurities in recycled PET</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_004458_gc_ms_impurities_recycled_pet_an004458_na_en_6cb2435671.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Polyethylene terephthalate (PET) is a widely used thermoplastic polymer valued for its strength, low weight, durability, and adaptability. Owing to these properties, it is extensively applied in packaging, textile fibers, and electronic components. PET is particularly suitable for recycling because it can be melted and reshaped multiple times with minimal loss of performance, and it is easily recognizable by the recycling code “1” (♳). The recycling of PET involves several stages, including collection, sorting, transport, processing, washing, pelletizing, and remanufacturing into new products [1]. However, during these steps, contaminants such as plasticizers, residual monomers, and degradation byproducts may be introduced.&nbsp;</p><p>The presence of such substances can pose potential risks to both environmental and human health, making the identification and characterization of contaminants in recycled PET essential for evaluating material quality and safety. Among the non-intentionally added substances (NIAS), benzene, acetaldehyde, and limonene are of particular relevance. Benzene is a recognized carcinogen and represents a serious health concern if present in PET. It may form as a degradation product of PET, with the reaction catalyzed by acidic compounds released at high temperatures by polymer impurities such as polyvinyl chloride (PVC). Poor temperature control or overheating can increase benzene formation as a degradation byproduct. Acetaldehyde, another degradation product of PET generated during bottle manufacturing, can alter the sensory properties of bottled beverages. Limonene, a flavor compound commonly present in soft drinks, may be absorbed into recycled PET and subsequently migrate into packaged beverages, potentially affecting product quality.&nbsp;</p><p>Gas chromatography coupled to headspace sampling is a fast and simple technique that enables the extraction of volatile and semivolatile compounds from solid and liquid samples without the need for time-consuming sample preparation.&nbsp;</p><p>In this study, headspace sampling coupled to single quadrupole mass spectrometry was used to assess the overall quantitative performance for analysis of acetaldehyde, benzene, and limonene in recycled PET packaging.&nbsp;</p><p>The capability of the Thermo Scientific™ ISQ™ 7610 single quadrupole GC–MS of combining the simultaneous acquisition of both single ion monitoring (SIM) and full scan (FS) allowed for sensitive targeted analysis as well as screening of unknown contaminants possibly present in recycled PET bottles.</p><h4><strong>Experimental&nbsp;</strong></h4><p>In all experiments, a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/64"><strong>TriPlus 500 HS autosampler</strong></a> was coupled to a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/486"><strong>Thermo Scientific™ TRACE™ 1610 Gas Chromatograph (GC)</strong></a>, configured with a Thermo Scientific™ iConnect™ Split/splitless Injector Module (iConnect-SSL), and connected to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/484"><strong>ISQ 7610 single quadrupole mass spectrometer</strong></a>.&nbsp;</p><p>The separation of the target analytes was achieved using a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1751"><strong>Thermo Scientific™ Trace GOLD™ TG-624 SILMS capillary column</strong></a>, 60 m x 0.25 mm x 1.4 µm (Part No. 26059-3330). This column provides a low to mid polarity phase with high thermal stability (maximum operating temperatures up to 320 °C) and high inertness, enabling Gaussian peak shapes for analysis of volatile organics. The TriPlus 500 HS autosampler is connected directly to the analytical column, removing the use of a long external transfer line. In this configuration, the SSL injector module is used only for the pneumatic control of the carrier gas and split flow.</p><h4><strong>Conclusions&nbsp;</strong></h4><p>The results presented in this work demonstrate that headspace coupled to GC–MS provides a suitable and simple solution for analysis of contaminants in rPET, with no complex sample preparation required.&nbsp;</p><ul><li>The capability of combining FS with SIM acquisition allows for qualitative and quantitative analysis, increasing confidence in compound identification through spectral comparison with NIST library and allowing for the detection of possible analyte co-elution.&nbsp;</li><li>The TriPlus 500 HS autosampler provides highly repeatable solventless extraction from solid samples with absolute area counts RSD &lt; 5%, permitting reliable and consistent quantitative data.&nbsp;</li><li>The ISQ 7610 single quadrupole GC–MS demonstrates an extended linear response (≥ 4–5 orders of magnitude) with R2 ≥ 0.997, calculated amounts within ± 20% the expected value, and ion ratio deviation ≤ 15% across the calibration curve.&nbsp;</li><li>Sensitivity was demonstrated with overall calculated LOD ≤ 0.03 ng/µL.</li><li>Chromeleon CDS helps ensure data integrity, traceability, and effective data management from instrument control to the final report streamlining analysis and data review processes.</li></ul></div></div></div></div></div></div></section></div></div></div></div></div></div></section>

News from LabRulezGCMS Library - Week 36, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

In 1936, the Laboratory Equipment Company introduced the first rapid carbon determinator for the American iron and steel industry.  Today, almost 80 years later, LECO is recognized globally as a leader in the development of high-quality analytical instrumentation, mass spectrometers, comprehensive two-dimensional chromatography, metallography & optical equipment as well as consumables.

LECO

Linde Oxygen 5.0 – Gas Cylinder

Linde Oxygen 5.0 (≥99.999%) gas cylinder for analytics, semiconductors, and research. Ultra-high purity for demanding applications.

<p><strong>Oxygen 5.0 from </strong><span><strong>Linde</strong></span><strong> is an ultra-high purity gas with a purity of ≥ 99.999%, supplied in pressurized cylinders for the most demanding laboratory, analytical, and industrial applications.</strong></p><p>It acts as a strong oxidizing agent in chemical processes and serves as an operational gas for analytical applications where maximum purity and minimal contamination are critical. Its quality ensures high precision and reproducibility.</p><p>Oxygen 5.0 is widely used in the chemical industry, semiconductor manufacturing, healthcare, and research and development environments.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Gas purity ≥ 99.999%</li><li>Ultra-high purity for demanding applications</li><li>Strong oxidizing properties</li><li>Suitable for analytical use</li><li>Minimal contamination levels</li><li>Supplied in pressurized cylinders</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> oxygen (O₂)</li><li><strong>Purity:</strong> ≥ 99.999% (5.0 grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Chemical industry (oxidation processes)</li><li>Semiconductor manufacturing</li><li>Analytical laboratories</li><li>Healthcare</li><li>Research and development</li></ul>

Linde Oxygen 6.0 – Gas Cylinder

Linde Oxygen 6.0 (≥99.9999%) gas cylinder for ultra-high purity applications in analytics, semiconductors, and research.

<p><strong>Oxygen 6.0 from </strong><span><strong>Linde</strong></span><strong> is an ultra-high purity gas with a purity of ≥ 99.9999%, supplied in pressurized cylinders for the most demanding analytical, semiconductor, and research applications.</strong></p><p>It functions as a strong oxidizing agent in chemical processes and as a high-purity operational gas for analytical use, where minimizing trace contaminants is essential. Its exceptional purity ensures maximum accuracy, stability, and reproducibility.</p><p>Oxygen 6.0 is particularly suited for advanced research, microelectronics, and other applications where gas quality is a critical parameter.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Gas purity ≥ 99.9999%</li><li>Ultra-high purity for critical applications</li><li>Strong oxidizing properties</li><li>Minimal impurity levels</li><li>Suitable for analytical and semiconductor applications</li><li>Supplied in pressurized cylinders</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> oxygen (O₂)</li><li><strong>Purity:</strong> ≥ 99.9999% (6.0 grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Chemical industry (high-purity oxidation processes)</li><li>Semiconductor manufacturing</li><li>Analytical laboratories</li><li>Healthcare</li><li>Research and development</li></ul>

Linde Helium 4.6 – Gas Cylinder

Linde Helium 4.6 (≥99.996%) gas cylinder for chromatography, leak detection, and industrial use. Reliable carrier and cooling gas.

<p><strong>Helium 4.6 from </strong><span><strong>Linde</strong></span><strong> is a high-purity inert gas with a purity of ≥ 99.996%, supplied in pressurized cylinders for a wide range of industrial and analytical applications.</strong></p><p>Due to its chemical inertness, low density, and excellent thermal conductivity, helium is ideal for cooling, protective atmospheres, and analytical workflows. It is widely used as a carrier gas in gas chromatography, ensuring stable separations and reproducible results.</p><p>Helium 4.6 is also applied in leak detection, metallurgy, glass production, laser technologies, and medical gas mixtures.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Gas purity ≥ 99.996%</li><li>Chemically inert</li><li>Excellent thermal conductivity</li><li>Low density</li><li>Ideal carrier gas for GC</li><li>Supplied in pressurized cylinders</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> helium (He)</li><li><strong>Purity:</strong> ≥ 99.996% (4.6 grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li><li>50 L (300 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Shielding gas in welding</li><li>Purging in metallurgy and specialty glass production</li><li>Cooling medium in reactors</li><li>Glass fiber manufacturing</li><li>Carrier gas in gas chromatography</li><li>Laser gas mixtures (with CO₂)</li><li>Leak detection</li><li>Medical gas mixtures</li></ul>

Linde Helium ECD – Gas Cylinder

Linde Helium ECD (≥99.999%) gas cylinder for GC-ECD analysis. Ultra-high purity carrier gas for trace-level detection and reliable results.

<p><strong>Helium ECD from </strong><span><strong>Linde</strong></span><strong> is an ultra-high purity inert gas with a purity of ≥ 99.999%, specifically designed for analytical applications in gas chromatography with electron capture detection (GC-ECD).</strong></p><p>Its extremely low impurity levels make it ideal for trace analysis, where baseline stability and minimal noise are critical. It serves as a carrier and operational gas in GC-ECD systems, commonly used for detecting halogenated compounds, pesticides, and persistent organic pollutants.</p><p>In addition to analytical use, helium ECD is also suitable for industrial applications such as purging in metallurgy, reactor cooling, glass fiber production, leak testing, and laser gas mixtures.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Gas purity ≥ 99.999%</li><li>Optimized for GC-ECD applications</li><li>Extremely low impurity levels</li><li>Stable baseline and low noise</li><li>Chemically inert</li><li>Supplied in pressurized cylinders</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> helium (He)</li><li><strong>Purity:</strong> ≥ 99.999% (ECD grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Carrier gas in gas chromatography (GC-ECD)</li><li>Analytical chemistry – trace-level analysis</li><li>Purging in metallurgy and specialty glass production</li><li>Cooling medium in reactors</li><li>Glass fiber manufacturing</li><li>Laser gas mixtures (with CO₂)</li><li>Leak detection</li><li>Medical gas mixtures</li></ul>

Linde Helium 5.3 – Gas Cylinder

Linde Helium 5.3 (≥99.9993%) gas cylinder for analytics, GC, and electronics. High-purity carrier and purge gas.

<p><strong>Helium 5.3 from </strong><span><strong>Linde</strong></span><strong> is a high-purity inert gas with a purity of ≥ 99.9993%, designed for demanding analytical applications and use in the electronics industry.</strong></p><p>It serves as an operational, carrier, and purge gas, particularly in analytical chemistry where high stability and low impurity levels are essential. Its physical properties, including low density and excellent thermal conductivity, make it ideal for chromatography, cooling, and protective atmospheres.</p><p>Helium 5.3 is widely used across industrial and research environments, including metallurgy, glass production, leak testing, laser applications, and medical gas mixtures.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Gas purity ≥ 99.9993%</li><li>Suitable for analytical applications and GC</li><li>Low impurity levels</li><li>Chemically inert</li><li>Excellent thermal conductivity</li><li>Supplied in pressurized cylinders</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> helium (He)</li><li><strong>Purity:</strong> ≥ 99.9993% (5.3 grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Carrier gas in gas chromatography</li><li>Analytical laboratories</li><li>Electronics industry</li><li>Purging in metallurgy and specialty glass production</li><li>Cooling medium in reactors</li><li>Glass fiber manufacturing</li><li>Laser gas mixtures (with CO₂)</li><li>Leak detection</li><li>Medical gas mixtures</li></ul>

Linde Helium 5.0 – Gas Cylinder

Linde Helium 5.0 (≥99.999%) gas cylinder for analytics, GC, and industry. High-purity carrier, purge, and measurement gas.

<p><strong>Helium 5.0 from </strong><span><strong>Linde</strong></span><strong> is a high-purity inert gas with a purity of ≥ 99.999%, suitable for a wide range of analytical, industrial, and research applications.</strong></p><p>It is commonly used as an operational, measurement, and purge gas and is an essential component of gas mixtures for welding and other industrial processes. Due to its chemical inertness, low density, and excellent thermal conductivity, it is ideal for gas chromatography, cooling applications, and protective atmospheres.</p><p>Helium 5.0 represents a versatile solution for laboratories and industrial environments where high purity, stability, and reliability are required.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Gas purity ≥ 99.999%</li><li>Suitable for analytical applications and GC</li><li>Chemically inert</li><li>Excellent thermal conductivity</li><li>Versatile use across industries</li><li>Supplied in pressurized cylinders</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> helium (He)</li><li><strong>Purity:</strong> ≥ 99.999% (5.0 grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Carrier gas in gas chromatography</li><li>Analytical laboratories</li><li>Purging in metallurgy and specialty glass production</li><li>Cooling medium in reactors</li><li>Glass fiber manufacturing</li><li>Laser gas mixtures (with CO₂)</li><li>Leak detection</li><li>Medical gas mixtures</li><li>Welding and shielding gas mixtures</li></ul>

Linde Helium 6.0 – Gas Cylinder

Linde Helium 6.0 (≥99.9999%) for GC, analytics, and research. Ultra-high purity carrier, purge, and measurement gas for demanding applications.

<p><strong>Helium 6.0 from </strong><span><strong>Linde</strong></span><strong> is an ultra-high purity inert gas with a purity of ≥ 99.9999%, designed for the most demanding analytical, scientific, and industrial applications.</strong></p><p>Its extremely low impurity levels make it particularly suitable for highly sensitive analytical techniques such as gas chromatography, where even trace contaminants can impact results. Helium 6.0 ensures excellent stability, reproducibility, and reliability when used as a carrier, purge, or measurement gas.</p><p>Thanks to its chemical inertness and high thermal conductivity, it is widely used across applications ranging from analytical chemistry to laser technologies and medical gas mixtures.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>Ultra-high purity ≥ 99.9999% (6.0 grade)</li><li>Extremely low impurity levels</li><li>Ideal for high-sensitivity analytical methods (GC)</li><li>Chemically inert</li><li>High thermal conductivity</li><li>Reliable and reproducible performance</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Gas:</strong> helium (He)</li><li><strong>Purity:</strong> ≥ 99.9999% (6.0 grade)</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Carrier gas in gas chromatography (GC)</li><li>Analytical and research laboratories</li><li>Purging in metallurgy and specialty glass production</li><li>Cooling medium in reactors</li><li>Glass fiber manufacturing</li><li>Laser gas mixtures (e.g., with CO₂)</li><li>Leak detection</li><li>Medical gas mixtures</li></ul>

Linde Helium Mixture (40% H₂ in He) – Gas Cylinder

Linde 40% H₂ in He gas mixture for GC and analytical applications. Stable, high-performance carrier gas for laboratory workflows.

<p><strong>The helium mixture (40% hydrogen in helium) from </strong><span><strong>Linde</strong></span><strong> is a specialty gas blend designed primarily for analytical and laboratory applications.</strong></p><p>The mixture is colorless, odorless, non-toxic, and classified as an asphyxiant. The combination of helium and hydrogen provides advantageous physical properties such as high thermal conductivity and low viscosity, supporting stable and efficient operation of analytical systems.</p><p>It is commonly used as a carrier or working gas in analytical techniques where optimized separation performance and signal stability are required.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>40% hydrogen in helium mixture</li><li>Colorless and odorless</li><li>Non-toxic, asphyxiant gas</li><li>High thermal conductivity</li><li>Stable composition</li><li>Suitable for analytical applications</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Composition:</strong> 40% hydrogen (H₂), 60% helium (He)</li><li><strong>Gas type:</strong> gas mixture</li><li><strong>Supply form:</strong> pressurized cylinder<ul><li>10 L (200 bar)</li><li>50 L (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Analytical chemistry</li><li>Gas chromatography (GC)</li><li>Laboratory measurements</li><li>Calibration and working gas</li><li>Research and development</li></ul>

Linde Helium/Nitrogen Mixture (10% He in N₂) – Gas Cylinder

Linde 10% He in N₂ gas mixture for leak detection. Safe, non-flammable, and stable gas for industrial and laboratory testing.

<p><strong>The helium/nitrogen mixture (10% helium in nitrogen) from </strong><span><strong>Linde</strong></span><strong> is a specialty gas designed primarily for leak detection applications in industrial and laboratory environments.</strong></p><p>The mixture is colorless, odorless, non-toxic, and non-flammable, but classified as an asphyxiant. The helium component enables highly sensitive leak detection using helium leak detectors due to its low molecular weight and high diffusivity, while nitrogen acts as an inert carrier gas.</p><p>Its stable composition and safe handling characteristics make it suitable for both routine and demanding leak testing applications across various industries.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>10% helium / 90% nitrogen mixture</li><li>Colorless and odorless</li><li>Non-toxic and non-flammable</li><li>Asphyxiant gas</li><li>Ideal for leak detection</li><li>Stable and consistent composition</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Composition:</strong> 10% helium (He), 90% nitrogen (N₂)</li><li><strong>Gas type:</strong> gas mixture</li><li><strong>Cylinder pressure:</strong> up to 300 bar</li><li><strong>Cylinder volume:</strong> 12 L (SV 12LH)</li><li><strong>Safety classification:</strong> H280 – contains gas under pressure; may explode if heated</li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Leak detection testing</li><li>Industrial processes</li><li>Manufacturing quality control</li><li>Laboratory testing</li><li>Maintenance and diagnostics</li></ul>

Linde Helium/Nitrogen Mixture (50% He in N₂) – Gas Cylinder

Linde 50% He in N₂ gas mixture for analytical and testing applications. Stable, non-flammable gas for laboratory and industrial use.

<p><strong>The helium/nitrogen mixture (50% helium in nitrogen) from </strong><span><strong>Linde</strong></span><strong> is a versatile gas blend designed for analytical, laboratory, and industrial applications.</strong></p><p>This mixture is colorless, odorless, non-toxic, and non-flammable. The combination of helium and nitrogen provides balanced physical properties, making it suitable for both analytical purposes and testing, calibration, or process applications.</p><p>The higher helium content compared to standard mixtures enhances detection sensitivity in leak testing and expands its applicability in analytical workflows where stability and reproducibility are essential.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>50% helium / 50% nitrogen mixture</li><li>Colorless and odorless</li><li>Non-toxic and non-flammable</li><li>Balanced physical properties</li><li>Suitable for analytical and industrial use</li><li>Stable composition</li></ul><h4><span><strong>Technical Specifications</strong></span></h4><ul><li><strong>Composition:</strong> 50% helium (He), 50% nitrogen (N₂)</li><li><strong>Gas type:</strong> gas mixture</li><li><strong>Supply form:</strong> pressurized gas cylinder<ul><li>50 l (200 bar)</li></ul></li></ul><h4><span><strong>Applications</strong></span></h4><ul><li>Analytical chemistry</li><li>Gas chromatography (specific applications)</li><li>Leak detection</li><li>Calibration gases</li><li>Laboratory testing</li><li>Industrial processes</li></ul>

Products from category Gases in pressure gas cylinders - page 2

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