A team from CEITEC Masaryk University (MUNI) in collaboration with researchers from two research institutes in Norway has, for the first time, captured how echovirus 18, a member of the enterovirus genus, releases its genetic material into cells to initiate infection. The study provides a structural view of an early stage of viral infection under near-native conditions. Using cryo-electron microscopy, the researchers discovered that the virus does not release its RNA through a small pore in its capsid, as had long been assumed. Instead, its protein shell partially disassembles, creating a large opening through which the viral genetic material escapes into the host cell. A detailed understanding of this genome release mechanism could eventually help develop compounds preventing enteroviruses from infecting cells.Enteroviruses are common human pathogens causing diseases ranging from the common cold and gastrointestinal infections to severe neurological disorders. Echovirus 18, the virus investigated in this study, is known primarily as a cause of meningitis and encephalitis in children. It belongs to the picornaviruses, a family of small, non-enveloped viruses whose genetic material is protected by a protein shell, the capsid, organized with icosahedral symmetry.To replicate within a cell, the enteroviruses must release their RNA – the molecule that carries viral genetic information – from the capsid, into the cell cytoplasm. Although this step is crucial for initiating infection, it has never been observed in infected cells.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vedci_z_CEITEC_jako_prvni_zachytili_jak_enteroviry_spousteji_infekci_bunek_71d553bb0e.jpg" alt="CEITEC/Plevka Lab: CEITEC Scientists Are the First to Capture How Enteroviruses Initiate Infection: View into a cell infected by echovirus 18. Echovirus 18 is shown in green and yellow, ribosomes in violet, and actin filaments in cyan. Scale 50 nm. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vedci_z_CEITEC_jako_prvni_zachytili_jak_enteroviry_spousteji_infekci_bunek_71d553bb0e.jpg 221w," sizes="100vw" width="1600" height="1128">“For a long time, two main theories existed about how picornaviruses deliver their RNA genomes into cells. One proposed that the genome leaves the capsid through a small opening. The other, supported by earlier work from our laboratory, suggested that the genome escapes from partially disassembled capsids missing pentamers of capsid proteins. However, both models were based on experiments with virus particles outside cells. We wanted to go one step further and see what really happens during infection inside a cell,” says <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/liya-mukhamedova-ph-d/u89248?page=publication">Liya Mukhamedova</a>, the study’s first author from CEITEC MUNI.Using cryo-electron microscopy, which allows scientists to visualize biological structures at exceptionally high resolution, the researchers observed individual echovirus 18 particles directly within infected cells. They discovered that empty virus particles in infected cells lack parts of their capsids. It is through these openings that the virus releases its RNA.The study also revealed the role of the FcRn receptor, a cell-surface molecule that enteroviruses use to enter cells. The binding of the virus to the receptor triggers structural changes in the viral particle that prime it for subsequent genome release. The team showed that receptor binding causes the loss of the so-called pocket factor, a small molecule that normally helps stabilize the virus particle. The virus otherwise remains largely intact, suggesting that receptor binding is only the first step toward uncoating, while additional factors within the cell are required to complete the process and trigger genome release.“Infection is a complex process, during which enterovirus particles sense various interactions with cell components. The binding of echovirus 18 to the FcRn host-cell receptor triggers conformational changes in the virus, priming it for genome release.” – explains <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/pavel-plevka-ph-d/u26854">Pavel Plevka</a>, head of the Structural Virology Research Group.The researchers did not observe the so-called activated particles that are key intermediates in the enterovirus uncoating process. This suggests that genome release in cells occurs much faster than previous experiments had suggested.By combining structural data obtained from studies of virus particles outside living cells with observations of infected cells, the study offers a picture of how enterovirus infection begins. Moving beyond simplified experiments with isolated virus particles, it brings scientists closer to understanding the earliest stages of enterovirus infection as they occur in living cells. The study, was published in the <a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1073/pnas.2601182123">PNAS journal</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vedci_z_CEITEC_jako_prvni_zachytili_jak_enteroviry_spousteji_infekci_bunek_2_3601bf1df9.jpg" alt="CEITEC/Plevka Lab: CEITEC Scientists Are the First to Capture How Enteroviruses Initiate Infection: Reconstructions of E18 particles from infected cells. The particles are rainbow-colored based on the distance from the particle center. Scale 10 nm." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vedci_z_CEITEC_jako_prvni_zachytili_jak_enteroviry_spousteji_infekci_bunek_2_3601bf1df9.jpg 245w," sizes="100vw" width="1600" height="906"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.pnas.org/doi/10.1073/pnas.2601182123">Particles of echovirus 18 open to release their genomes in vivo</a></h4>Liya Mukhamedova, David Buchta, Zuzana Trebichalská, Yevgen Levdansky, Jana Moravcová, David Potěšil, Zbyněk Zdráhal, Dominik Hřebík, Lucie Nepovímová, Torleif Tollefsrud Gjølberg, Jan Terje Andersen, Jiří Nováček, Tibor Füzik, and Pavel PlevkaProc. Natl. Acad. Sci. U.S.A., 2026, 123, e2601182123<div>&nbsp;</div><div><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1073/pnas.2601182123">https://doi.org/10.1073/pnas.2601182123</a></div><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Enteroviruses cause a broad spectrum of human diseases, ranging from mild respiratory or gastrointestinal infections to severe neurological disorders such as aseptic meningitis and encephalitis. Enterovirus cell entry involves receptor-mediated endocytosis followed by destabilizing rearrangements of the virus capsid that enable genome release. However, the mechanism of enterovirus genome release has not been visualized in infected cells. Here, we used cryoelectron tomography and microscopy to image echovirus 18 (E18) entry into host cells and its interaction with the neonatal Fc receptor (FcRn). 30 min postinfection, endosomes and cytoplasm contained empty capsids missing one or several pentamers of capsid proteins, providing evidence that in vivo E18 releases its genome through capsid opening. In vitro, FcRn binding induced the expulsion of pocket factors from hydrophobic pockets in VP1, priming the virus for uncoating. The cryoelectron microscopy reconstruction of genome-containing particles of E18 inside infected cells did not reveal pocket factors, indicating that receptor binding triggers the same priming process during infection. We did not detect activated particles in infected cells, suggesting that these intermediates are short-lived and rapidly release their genomes in vivo. Our results identify capsid opening as the in vivo mechanism of echovirus 18 genome release, providing structural evidence for a process previously only inferred from in vitro experiments.

CEITEC Scientists Are the First to Capture How Enteroviruses Initiate Infection 

Gas chromatography (GC) is a powerful analytical technique used to separate and analyze compounds in a mixture. Whether you're working in pharmaceuticals, environmental testing, or food safety, understanding the key components of a gas chromatograph is essential for accurate and efficient results.In this article, we will delve into the primary components of gas chromatography, including the injector, column, detector, and carrier gas—and explain how each plays a critical role in the separation and analysis process.By mastering these elements, you can optimize your GC setup for precise and reliable outcomes. Let's explore each component in detail:<h3>Sample Injector</h3>The injector is the starting point in the <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/gc">gas chromatography process</a>, where the sample is introduced into the system. In GC, the sample must be vaporized into a gas before it enters the column. The injector serves this purpose by heating the sample to its gaseous state. There are many types of injectors, Split/Splitless, PTV, on column.The most common injector is the Split/Splitless injector that can be used in split mode or in splitless mode:<ul><li>Split Injection: This mode allows a portion of the sample to be sent to the column while the remainder is vented. It is useful for high-concentration samples to prevent system overload.</li><li>Splitless Injection: In this mode, the entire sample is introduced into the column, making it ideal for trace analysis where even minute quantities need detection.</li></ul>Proper choice of injector type and proper control of injection conditions, such as temperature, sample size, injection mode are crucial for achieving reproducible and reliable results.<h3>Carrier Gas</h3>In gas chromatography, the mobile phase is represented by carrier gas, which transports the vaporized sample through the column. The carrier gas is an inert gas, such as helium, nitrogen, or hydrogen. The choice of carrier gas can influence the efficiency of the separation process.<ul><li>Helium is the most used carrier gas due to its inertness and high flow rate.</li><li>Hydrogen offers faster analysis times but requires careful handling due to its flammability.</li><li>Nitrogen provides a cheaper solution compared to Helium, although it's slower than helium or hydrogen.</li></ul>The flow rate and pressure of the carrier gas need to be carefully controlled to ensure that the sample moves smoothly through the system and adequate efficiency is generated.<h3>Column/Stationary Phase</h3>The column is the heart of the gas chromatograph, where the actual separation of compounds occurs. <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/products?category=7&amp;manufacturerItems=50">GC columns</a> are typically long, thin tubes made from stainless steel or fused silica. They are coated with a stationary phase, which interacts with the components of the sample as they pass through.<ul><li>Packed Columns: These contain small particles of stationary phase material and generally have a larger volume. While they are less efficient than capillary columns, they can be suitable for specific applications.</li><li>Capillary Columns: These thin tubes are coated with a stationary phase, offering higher resolution and faster analysis times due to their smaller diameter.</li></ul>The length, diameter, and type of stationary phase of the column are important factors that affect the efficiency and selectivity of the separation. The choice of stationary phase (polar or non-polar; liquid-coated or solid) affects how different compounds interact with it.As the sample moves through the column, compounds separate based on volatility and their affinity for the stationary phase, resulting in distinct peaks in the chromatogram.<h3>Detector</h3>Once the sample has passed through the column, it reaches the detector, where individual components are identified and measured. There are several types of detectors, each suited to different applications:<ul><li>Flame Ionization Detector (FID): One of the most commonly used detectors in GC. It measures ions produced when organic compounds are burned in a hydrogen-air flame. FID is highly sensitive to a wide range of organic compounds and offers a broad linear dynamic range.</li><li>Thermal Conductivity Detector (TCD): Detects changes in the thermal conductivity of the gas stream as analytes elute from the column. It is a universal, non-destructive detector, but is generally less sensitive than FID.</li><li>Electron capture detector (ECD): A very sensitive and selective detector used to detect chlorinated compounds it is easily contaminated and requires very pure and dry carrier and makeup gases and difficult maintenance.</li><li>Mass Spectrometer (MS): Provides structural information, aiding in both identification and quantification. Requires advanced and trained users and it’s difficult to maintain.</li></ul>The choice of detector depends on the type of analysis being performed and the sensitivity required. Each detector converts physical or chemical changes associated with the analytes into a measurable signal, producing a chromatogram that reflects concentration and retention time.<h3>Data System</h3>Although not a physical part of the chromatograph, the data system plays an integral role in processing and interpreting the signals generated by the detector. Modern gas chromatographs are equipped with software that converts detector signals into chromatograms—visual representations of the separation process. The software allows users to quantify and identify the components of the sample based on retention times and peak areas.<h3>How to Read a Gas Chromatogram?</h3>Interpreting a gas chromatogram involves understanding its essential elements, such as peaks, retention times, and the overall data for qualitative and quantitative analysis.<h4>The Nature of the Sample</h4>Begin by considering the sample's characteristics; different samples, such as environmental pollutants or food components, may exhibit distinct peak patterns. Recognizing the baseline (the flat line representing the signal without analytes) is crucial for identifying peaks accurately. While well-defined peaks indicate successful separation of components, poorly shaped or broad peaks may suggest issues with the separation process. For instance, if a mixture is injected and three peaks are observed, it indicates the presence of three different compounds in the sample. Conversely, when evaluating the purity of a sample, one would anticipate observing a single peak. Ideally, the chromatogram should reflect this expectation, confirming the sample's purity.<h4>The Identity of the Sample</h4>Retention time (Rt) is a critical factor in identifying the compounds present in the sample. Each analyte has a characteristic retention time that can be compared to known standards or literature. To confirm identities, a standard mixture should run alongside the sample; this allows to correlate retention times with specific compounds. If available, utilizing MS in conjunction with gas chromatography (GC-MS) can provide additional structural information, enhancing identification accuracy. With HR-MS or MS-MS detectors, unknown compounds can be identified by matching perfectly the Molecular weight or by matching the fragmentation pattern.<h4>The Amount of Sample</h4>Quantifying analytes involves analyzing peak area or height, which is directly proportional to the concentration of the analyte in the sample. A calibration curve constructed from known concentrations can help relate peak areas to actual concentrations. It is essential to consider the detector’s sensitivity and the limits of detection, as peaks below a certain threshold may not provide reliable quantification.

Basic Principles of Gas Chromatography - Separation and Analysis

ISC 2026 - Day 2

Article

Events

Academy

Science and research

Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Agilent Technologies

Technology Networks

Thermo Fisher Scientific

SelectScience

SCIEX

KNAUER

SciY/Mestrelab Research

Waters Corporation

<div>As monoclonal antibody (mAb) production titers continue to rise, downstream purification is becoming a critical bottleneck for many development and manufacturing teams. Traditional bind-and-elute cation exchange (CEX) polishing steps can limit capacity and process efficiency, creating a growing need for higher-productivity alternatives.Join this webinar to explore how flow-through hydrophobic interaction chromatography (HIC) can help overcome these challenges by enabling improved loading capacity and streamlined purification.Through a real-world development case study, attendees will gain practical insights into resin evaluation, process optimization, and successful scale-up strategies that support efficient technology transfer and manufacturing readiness.<h5>Attend this webinar to:</h5><ul><li>Understand mAb polishing capacity constraints and opportunities</li><li>Discover frameworks that can replace CEX bind-and-elute steps with HIC flow-through</li><li>Learn from a detailed case study of HIC resin selection and process development</li><li>Confirm scale up conditions and support consistent performance for manufacturing</li></ul><blockquote>Presenter: Andrew Siemers (Systems Design Engineer at Thermo Fisher Scientific)Andrew Siemers is a systems design engineer at Thermo Fisher Scientific, focusing on downstream bioprocessing research and development. Andrew works as a part of the Bioprocessing Collaboration Center in St. Louis, Missouri which works to develop new products and processes across the downstream train. He specializes primarily in monoclonal antibody workstreams, with focuses on resin development and implementation as well as directly supporting the on-site contract development and manufacturing organization.</blockquote></div>

Technology Networks: Improving mAb Purification Efficiency With Hydrophobic Interaction Chromatography

Improving mAb Purification Efficiency With Hydrophobic Interaction Chromatography

Discover how flow-through HIC improves mAb polishing, increases purification capacity, and supports efficient downstream bioprocessing.

mAb Purification With Hydrophobic Interaction Chromatography

The challenge of developing and maintaining robust chemometric models for use within Process Analytical Technology (PAT) deployment remains a significant barrier to implementation. This webinar explores how Iterative Optimization Technology (IOT) is advancing the principles of lean chemometrics by reducing the calibration burden associated with PAT deployment. Case studies will demonstrate how these methods enable faster model deployment, improved lifecycle management, and greater operational efficiency without compromising product quality or process understanding. Attendees will gain insights into how innovative chemometric strategies can accelerate PAT adoption while supporting the industry's drive towards agile, data-driven manufacturing.<h4>Learning Points</h4><ul><li>You will learn how lean chemometrics reduces model complexity and data requirements</li><li>Discover how to simplify model deployment and lifecycle management</li><li>Explore how to accelerate PAT adoption while maintaining process understanding and quality</li></ul><h4>Who should attend</h4><ul><li>Process development and MSAT teams scaling processes to manufacturing</li><li>PAT scientists and analytical experts developing chemometric models</li><li>Lab managers driving efficient and scalable PAT implementation</li><li>Development and manufacturing and digitalization leaders advancing data-driven operations</li></ul><blockquote>Presenter: Jonique Samuels (Scientific Advisor for Innovations, SciY)Jonique Samuels is a Scientific Advisor for Innovations at SciY where she leads research and development projects on new, novel functionality for synTQ, the orchestration and real-time process-control platform that enables PAT and advanced, quality-centric control strategies. Currently, Jonique's focus is on supporting sustainability initiatives with customers, which includes investigating and developing lean chemometrics methods.&nbsp;</blockquote><blockquote>Presenter: Anna Codina, Ph.D. ( Senior Director Strategy and Business Development, SciY)Anna Codina is Senior Director of Strategy and Business Development at SciY, where she focuses on enabling data-driven orchestration across the pharmaceutical value chain. With a background in experimental science, analytical development, and digital innovation, she works at the intersection of data, automation, and AI to accelerate scientific workflows and decision-making.Anna holds a Ph.D. in protein NMR from the University of Barcelona and completed postdoctoral research at the MRC Laboratory of Molecular Biology in Cambridge. She spent seven years in Analytical R&amp;D at Pfizer, specializing in reaction monitoring, impurity characterization, and regulatory submissions, and was recognized with a Pfizer Worldwide Achievement Award for implementing NMR-based reaction monitoring in an open-access environment.Since joining Bruker, Anna has held multiple leadership roles across applications, product management, and biopharma business development and market management. In her current role at SciY, she drives strategy for vendor-agnostic data platforms and digital solutions that connect instruments, robotics, and enterprise systems enabling orchestration, AI-powered experimentation and scalable workflows across R&amp;D, CROs/CDMOs, and manufacturing.</blockquote>

SciY: Advancing Lean Chemometrics: Iterative Optimization Technology as a Process Analytical Technology Tool for Reducing Calibration Burden

Advancing Lean Chemometrics: Iterative Optimization Technology as a Process Analytical Technology Tool for Reducing Calibration Burden

Reduce PAT calibration burden with Lean Chemometrics and IOT. Learn to accelerate model deployment and simplify lifecycle management in this webinar

Lean Chemometrics & IOT: Reducing PAT Calibration Burden

Simplifed, turnkey workflows of the Agilent 8700 LDIR.<blockquote>Presenter: Louis Tisinger, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)Louis (Lou) Tisinger has a Ph.D. in Analytical Chemistry from Miami University in Oxford Ohio, where his graduate research involved the study of ATR imaging and microscope performance in vibrational spectroscopy. Most of his career has been related to pre- and post-sales support of FTIR spectrometers, microscopes, imaging systems, and thermal analytical instruments, focusing on analyzing customer samples, demonstrations, post-sales training, troubleshooting, and R&amp;D.</blockquote>

Agilent Technologies: IR Microscopy to Streamline Your Microplastics Analysis

IR Microscopy to Streamline Your Microplastics Analysis

discover streamlined microplastics analysis using IR microscopy. Learn how the Agilent 8700 LDIR system provides automated workflows for fast testing

Agilent 8700 LDIR: Streamlined Microplastics Analysis

A live webinar hosted by Life Science Connect, presented by KNAUER in partnership with BIZON Labs (a spin-off of an MIT research project)Lipid nanoparticles are now central to the delivery of genetic medicines, yet LNP process development remains empirical and resource-intensive. Identifying process parameters for scalable, reproducible, and robust manufacturing is challenging, as particle quality attributes are highly sensitive to formulation and processing conditions.This webinar explores how impinging jets mixing can be integrated with automation, inline analytics, and data-driven optimization to accelerate LNP process development. Attendees will see how automated design of experiments, dynamic process sweeps, self-optimization, and predictive modeling can map complex process-property relationships and rapidly identify conditions that achieve target quality attributes.We'll show how closed-loop experimentation can transform LNP development from an empirical workflow into a faster, more resource-sparing process, and discuss the challenges that remain on the path to fully autonomous nanoparticle manufacturing.<h5>What you'll learn:</h5><ul><li>How impinging jets mixing parameters influence LNP critical quality attributes such as size, structure, and morphology</li><li>How automation enables faster, data-rich LNP process development</li><li>How design of experiments, dynamic sweeps, self-optimization, and data-driven modeling support predictive and scalable LNP manufacturing</li></ul><h5>Who should attend:</h5><ul><li>Formulation scientists working on RNA, mRNA, siRNA, or gene therapy delivery</li><li>Process development scientists and engineers in nanoparticle manufacturing</li><li>CMC, MSAT, and manufacturing scientists supporting LNP scale-up</li><li>R&amp;D leaders evaluating automated or data-driven approaches to drug delivery development</li></ul><blockquote>Speaker: Peter Sagmeister, PhD (BIZON Labs, spinning out of MIT) Peter Sagmeister earned his BSc and MSc from the University of Graz, Austria, and completed his PhD there in 2022 under Prof. Oliver Kappe, focusing on the digitalization of continuous flow chemistry for APIs and intermediates. During his postdoctoral work at the University of Graz and RCPE GmbH, he published extensively on process analytical technology, data science, and machine learning for small molecule process development. He is currently a Postdoctoral Associate in the Department of Chemical Engineering at MIT, where his work focuses on modernizing pharmaceutical manufacturing. Peter has collaborated with leading pharmaceutical companies, including Roche, Boehringer Ingelheim, Takeda, and AstraZeneca, to develop manufacturing platforms and data-driven workflows spanning small molecules to mRNA therapeutics.</blockquote><blockquote>&nbsp;Speaker: Dr. Nadia Elghobashi-Meinhardt (KNAUER)&nbsp;Nadia Elghobashi-Meinhardt received a BS in Chemistry from Stanford University in 1998 and a PhD in Chemistry in 2005 from the Freie Universität Berlin. After completing postdoctoral work in computational biophysics at the Fritz-Haber-Institut Berlin, Heidelberg University, and the Technical University Berlin, Nadia joined the School of Chemistry at University College Dublin (Ireland) as Assistant Professor in 2023. At KNAUER, Nadia serves as Principal Consultant and Technical GTM in the Customized Solutions team supporting LNP projects.</blockquote>

KNAUER: Autonomous Impinging Jets Mixing for LNP Process Development

Autonomous Impinging Jets Mixing for LNP Process Development

Accelerate LNP process development with KNAUER & BIZON Labs. Learn how automated impinging jets mixing and closed-loop modeling optimize RNA delivery

Autonomous Impinging Jets Mixing in LNP Development

Analytical Strategies for GLP-1 Therapeutics: Advancing Impurity Profiling with LC-UV/MS and High Resolution MS&nbsp;Glucagon-like peptide-1 (GLP-1) receptor agonists have transformed the treatment of type 2 diabetes and obesity, while increasing molecular complexity has created new analytical challenges for pharmaceutical scientists. Sequence modifications, fatty acid conjugation, and emerging multi-agonist designs require robust analytical strategies capable of detecting, characterizing, and monitoring impurities throughout development and manufacturing to ensure product quality, safety, and efficacy.<h4>Why you should attend</h4>This webinar will showcase complementary LC-MS approaches for the characterization and impurity profiling of GLP-1 therapeutics.Part 1 will highlight the use of LC-UV/MS workflows as a versatile toolbox for impurity analysis of exenatide. Case studies will demonstrate how orthogonal mass information can enhance impurity detection beyond UV-based analysis, while complementary chromatographic approaches, including reverse-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC), improve impurity resolution and raw material screening. Applications for characterizing amino acid sequence variants, monitoring stability-indicating impurities, and supporting risk-based decision-making in peptide manufacturing environments will be discussed.Part 2 will focus on advanced characterization of the tirzepatide using high resolution mass spectrometry (HRMS). Through forced degradation studies under oxidative stress conditions, HRMS will be used to confidently identify degradants and elucidate impurity pathways, providing detailed structural insights into sites of molecular vulnerability and degradation mechanisms. Case studies will demonstrate how HRMS supports comprehensive characterization of complex peptide therapeutics and helps establish the analytical methodologies needed to identify and control clinically relevant impurities.<h4>Key Learning Objectives</h4><ul><li>Apply LC-UV/MS workflows to characterize impurities, sequence variants, and stability-indicating degradants in GLP-1 therapeutics.</li><li>Leverage complementary RPLC and HILIC separations to improve impurity detection and analytical confidence.</li><li>Understand how HRMS enables detailed structural characterization of GLP-1 analogs and their impurities.U se forced degradation studies and degradant identification to support stability assessment and impurity control strategies.</li></ul><h4>Who should attend</h4>This webinar is designed for analytical development, LC-MS, CMC, and QC scientists working in pharmaceutical and biotechnology organizations developing or manufacturing GLP-1 and other therapeutic peptides.Don't miss it!<blockquote>Presenter: Duanduan Han, Ph.D. (Senior Scientist, Waters Corporation)Duanduan Han is a Senior Scientist at Waters Corporation in Milford, MA. She specializes in LC–MS method development and troubleshooting, impurity profiling, and raw material screening for biopharmaceutical products and new modalities, including GLP‑1 therapeutics, lipid nanoparticles, oligonucleotides, surfactants, and monoclonal antibodies.She earned her PhD in Chemical Engineering from Texas A&amp;M University.</blockquote><blockquote>Presenter: Christopher Henry (Senior Scientist, Waters Corporation)Chris Henry is a Principal Scientist at Waters Corporation in Wilmslow, UK. He has worked at Waters for 13 years focusing on LC-MS and GC-MS method development across small molecule and large molecule workflows using high resolution mass spectrometry.</blockquote>

GLP‑1 Analogs: Enhancing Impurity Profiling with LC‑UV/MS Workflows

Profile impurities in GLP-1 therapeutics using LC-UV/MS and HRMS. Learn RPLC/HILIC separation and forced degradation analysis for exenatide and tirzepatide

GLP-1 Impurity Profiling: LC-UV/MS & HRMS Workflows

<div>Bispecific antibodies can present purification challenges that push beyond established platform processes. When molecules “misbehave,” downstream process development scientists need practical strategies for identifying suitable chromatography resins, conditions, and operating windows.Join this data-driven presentation to explore process development resin screening for bispecific antibody purification.See how product-related impurity profiles and high-throughput screening of resins across various modalities (ion exchange, hydrophobic interaction, and mixed-mode cation exchange) can help guide resin selection and identify conditions that balance yield and purity.<h5>Attend this webinar to:</h5><ul><li>Discover how bispecific impurity profiles can guide resin selection</li><li>Learn a practical workflow for screening ion exchange chromatography, hydrophobic interaction chromatography, and mixed-mode cation exchange chromatography resins</li><li>Explore how selectivity mapping can reveal promising operating windows</li><li>See how screening results can support scale-up to column confirmation runs</li></ul><blockquote>Presenter: Michelle Nolasco (Systems Design Engineer, Thermo Fisher Scientific)&nbsp;Michelle Nolasco leads innovation initiatives and collaborations for bioproduction at Thermo Fisher Scientific. Michelle specializes in downstream processes and has experience in automation and advanced analytics.&nbsp;</blockquote></div>

Technology Networks: Tackling Bispecific Antibody Purification: A Polishing Case Study

Tackling Bispecific Antibody Purification: A Polishing Case Study

Optimize bispecific antibody purification and resin screening. Learn how IEX, HIC, and mixed-mode chromatography improve yield, purity, and scale-up

Bispecific Antibody Purification: Resin Screening

Complex sample matrices can make trace element analysis challenging, particularly when spectral interferences limit confidence in results. In applications such as mining analysis, conventional ICP-OES approaches may not always provide the resolution needed to clearly separate overlapping signals or achieve the detection limits required for demanding workflows.In this 20-minute TechTalk, discover how high-resolution ICP-OES can help overcome spectral interferences, improve confidence in elemental analysis, and deliver reliable results for complex samples. The session will show how enhanced spectral resolution, combined with strong sensitivity, can support more robust trace element detection where conventional approaches may reach their limits.Designed for ICP-OES users, elemental analysis specialists, analytical chemists, method developers, laboratory managers, QC/QA professionals, and researchers working with complex sample matrices, this session will provide practical insight into how high-resolution ICP-OES can strengthen analytical performance and support confident decision-making in challenging applications.<h5>Certificate of attendance</h5>If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.<h5>TechTalk details</h5>&nbsp; &nbsp;Cost: Free to attend &nbsp; &nbsp;Location: Online &nbsp; &nbsp;Duration: 20 minutesRegistration is required to secure your place. If you register but can’t attend live, you will receive a link to the on-demand recording once it becomes available.<h5>Who should attend?</h5>This event is perfect for:<ul><li>ICP-OES users</li><li>Elemental analysis specialists</li><li>Analytical chemists and method developers</li><li>Laboratory managers and quality managers</li><li>QC/QA professionals</li><li>Researchers working with complex sample matrices</li><li>Scientists evaluating elemental analysis technologies</li></ul><h5>What will this webinar cover?</h5>Key learning objectives:<ul><li>Understand how high-resolution ICP-OES helps resolve spectral interferences in complex samples.</li><li>Explore application examples where conventional ICP-OES approaches may reach their limits.</li><li>Discover how high resolution combined with best sensitivity enables extraordinary detection limits</li></ul><blockquote>Presenter: Dr. Kilian Schneider (Product Specialist ICP-OES, Analytik Jena GmbH+Co.KG)Dr. Kilian Schneider is a Product Specialist for ICP-OES at Analytik Jena, based in Jena, Germany. He specializes in elemental analysis and supports customers worldwide in the development and optimization of analytical methods for challenging applications. His expertise covers a broad range of industries, including mining, environmental analysis, chemicals, advanced materials, energy, and recycling. Dr. Schneider is actively involved in application development, customer support, technical training, and the introduction of innovative ICP-OES solutions for complex analytical challenges.</blockquote>

SelectScience: TechTalk - Benefits of high-resolution ICP-OES for challenging applications

TechTalk: Benefits of high-resolution ICP-OES for challenging applications

Learn how high-resolution ICP-OES from Analytik Jena overcomes spectral interferences and improves trace element analysis in complex sample matrices

High-Resolution ICP-OES for Complex Sample Matrices

Synthetic peptides like GLP-1 analogs (e.g., semaglutide, tirzepatide) present analytical challenges due to complex modifications and potential degradation products. High resolution accurate mass spectrometry (HRMS) offers the sensitivity, resolution, and mass accuracy needed for confident sequence confirmation and impurity profiling.In this Tech Talk, attendees will discover how integrated workflows from high-throughput screening using the INTACT Mass App to detailed degradation characterization with UNIFI streamline synthetic peptide analysis, support quality attribute monitoring, and ensure regulatory compliance.<h5>Certificate of attendance</h5><ul><li>If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.&nbsp;</li><li>If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.</li></ul><h5>TechTalk details</h5><ul><li>Cost: Free to attend</li><li>Location: Online</li><li>Duration: 20 minutes</li></ul>Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on-demand recording once it becomes available.<h5>Who should attend?</h5>This event is perfect for:<ul><li>Bioanalytical scientists</li><li>Pharmaceutical R&amp;D researchers</li><li>QC/QA laboratory managers</li><li>Contract Research Organisation (CRO) scientists</li><li>Mass spectrometry specialists</li></ul><h5>What will this webinar cover?</h5><ul><li>The advantages of high resolution accurate mass spectrometry (HRMS) over nominal mass systems for resolving near-isobaric impurities and peptide variants.</li><li>How high-throughput screening tools enable rapid mass confirmation, sequence verification, and routine purity reporting.</li><li>Strategies for comprehensive qualitative analysis and tracking of forced degradation products.</li></ul><blockquote>Presenter: Jonathan Fox (Biologics Consulting Scientist, Waters Corporation)Jonathan Fox is a Biologics Consulting Scientist at Waters Corporation, specializing in advanced high resolution accurate mass spectrometry (HRMS) applications for biopharmaceutical research and development. With extensive expertise in biotherapeutic characterization, analytical chemistry, and liquid chromatography-mass spectrometry (LC-MS) workflows, he focuses on developing innovative solutions for complex molecules, including synthetic peptides, oligonucleotides, and therapeutic proteins. He works closely with pharmaceutical and biotech organizations to optimize quality attribute monitoring and impurity profiling strategies.</blockquote>

SelectScience: GLP-1 & GIP under the lens: HRMS strategies for synthetic peptide profiling

GLP-1 & GIP under the lens: HRMS strategies for synthetic peptide profiling

Explore HRMS workflows for synthetic peptide analysis, including sequence confirmation, impurity profiling, purity assessment, and degradation characterization.

GLP-1 & GIP HRMS strategies for synthetic peptide profiling

Join us for an insightful webinar exploring advanced LC-MS/MS approaches for the quantitative analysis of nitrosamine impurities using the SCIEX novus V55 system.This session will highlight highly sensitive and selective LC-MS/MS workflows for the detection and quantitation of trace-level nitrosamine impurities in pharmaceutical products. Learn about optimized sample preparation, chromatographic separation, and MRM-based quantitation strategies designed to deliver accurate, reproducible, and regulatory-compliant results.The webinar will also cover method development and validation considerations, analytical challenges associated with nitrosamine analysis, and best practices to support global regulatory requirements for routine quality control and risk assessment.<blockquote>Speaker: Dr. Dev Kant Shandilya (Director - Analytical Research and Development, Fresenius Kabi)Dr. Devkant is an accomplished analytical chemistry leader with over 26 years of experience in pharmaceutical analytical R&amp;D, specializing in APIs, drug products, peptides, and complex generics. He has extensive expertise in LC-MS/MS, impurity profiling, nitrosamine and elemental impurity analysis, method development and validation, and regulatory submissions (DMF, NDA, and ANDA). Holding a Ph.D. in Chemistry, an MBA, and an M.Sc. in Analytical Chemistry, Dr. Devkant has successfully led high-performing analytical teams and driven innovation in pharmaceutical development and regulatory compliance.Topic: LC-MS-Based Quantitative Analysis of Nitrosamines from LeachableKey Highlights:<ul><li>Ultra-trace detection and quantitation of nitrosamines using highly sensitive LC-MS methodologies.</li><li>Optimized sample preparation and chromatographic separation for enhanced selectivity and analytical robustness.</li><li>Validated workflows supporting regulatory-compliant risk assessment of nitrosamine impurities arising from leachable sources.</li></ul>Speaker: Aman Sharma (Sales Development Manager, Sciex India Pvt Ltd)Aman Sharma has over 18 years of experience in analytical sciences, spanning analytical instrumentation, application support, and strategic marketing. He specializes in understanding market needs, driving demand generation, and developing innovative marketing initiatives. Known for his strategic mindset, customer-centric approach, and collaborative leadership, he has successfully bridged technical expertise with business growth.Topic: High-Sensitivity Quantitation of Nitrosamine Impurities with the SCIEX novus V55 systemKey Highlights:<ul><li>Enhanced sensitivity for low-level nitrosamine detection.</li><li>Robust quantitative performance with optimized LC-MS/MS workflows.</li><li>Streamlined method development for routine quality control and regulatory compliance.</li></ul></blockquote>

SCIEX: LC-MS/MS Analysis of Nitrosamines using novus V55

LC-MS/MS Analysis of Nitrosamines using novus V55

Explore LC-MS/MS workflows for trace nitrosamine analysis with the SCIEX novus V55, including MRM quantitation, validation, and regulatory compliance.

<h4>Microplastic analysis in complex matrices</h4>Join Thermo Fisher Scientific and Frontier Laboratories for a two-week webinar series exploring how Pyrolysis-GC-MS (Py-GC-MS) is helping laboratories solve some of today's most challenging analytical problems. Learn how Py-GC-MS workflows are being applied to emerging environmental contaminants, regulatory compliance, food safety, and advanced materials characterization.In this webinar, discover how pyrolysis GC Orbitrap MS enables confident identification and quantification of microplastics in complex food and environmental samples. Learn how streamlined workflows, enhanced selectivity, and targeted and non-targeted analysis support reliable results across challenging matrices, including fishmeal, stormwater, milk, and meat.<h5>What you will learn:</h5><ul><li>The advantages of Py-GC-MS for polymer identification, regardless of particle size</li><li>The role of high-resolution accurate-mass technology in improving selectivity, quantitation, and unknown identification</li><li>Best practices for applying targeted and non-targeted workflows to complex food and environmental samples</li></ul><blockquote>Speaker:&nbsp;Dominic Roberts (Product Marketing Manager, GC-MS, Thermo Fisher Scientific)Dominic Roberts has supported the Thermo Scientific GC Orbitrap MS technology since its launch in 2015. He joined Thermo Fisher Scientific as a Senior Applications Specialist and is currently the GC Orbitrap MS Product Marketing Manager. An analytical chemist and mass spectrometrist with 25+ years’ experience, he has worked with GC–MS and LC–MS across food, biological, and environmental analysis.Moderator: Daniela Cavagnino (Product Marketing Manager GC and Sampling Solutions, Thermo Fisher Scientific)Dr. Daniela Cavagnino earned an MS in Chemistry from the University of Milan. After starting in GC R&amp;D and applications at Thermo Fisher Scientific, she moved into product and marketing management. She has 25+ years of experience in GC/GC-MS for industrial and applied markets, specializing in advanced sampling and automated workflow solutions.</blockquote>

Thermo Fisher Scientific: From Microplastics and PFAS to Advanced Materials

Confident Microplastics Analysis in Food with Py-GC Orbitrap MS

Explore Py-GC Orbitrap MS workflows for microplastics analysis in food and environmental samples with accurate-mass targeted and non-targeted analysis.

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