<p><strong>Microplastics have emerged as one of the most widely discussed environmental contaminants of our time. Regardless of where we live, daily exposure to microplastics has become virtually unavoidable—these tiny plastic particles are present in the air we breathe, the water we drink, and the environments we inhabit. As researchers work to better understand the sources, transport pathways, and impacts of microplastics, the demand for reliable microplastic analysis methods continues to grow.</strong></p><p>While techniques such as FTIR and Raman spectroscopy have become standard tools for identifying microplastics, their utility is limited for smaller particles (i.e. nanoplastics) and in complex matrices. More advanced microplastic analysis methods can provide a deeper understanding of what these particles contain and how they may change over time in the environment.</p><div><h3><strong>The Limits of Spectroscopic Microplastic Analysis Methods</strong></h3><p><strong>Fourier-Transform Infrared</strong> (<strong>FTIR</strong>) and <strong>Raman spectroscopy </strong>are widely used microplastic analysis methods because they can<strong> identify polymer types</strong> by measuring how electromagnetic radiation interacts with a material. These techniques are valuable for determining whether a particle is polyethylene, polypropylene, polystyrene, or another common plastic. For many studies, this information is sufficient to confirm the presence of microplastics, especially in clean samples with sufficient microplastic density.</p><p>However, <a target="_blank" rel="noopener noreferrer" href="https://www.leco.com/application/environmental/"><strong>environmental samples</strong></a> are rarely clean and compositionally simple. Microplastic particles can become weathered and obscured by other materials in the environment, making their accurate identification with spectroscopy difficult. Additionally, if researchers want to understand what additives, degradation products, or pollutants are associated with the plastic, particle identification alone is not enough. Thus, FTIR and Raman spectroscopy methods alone struggle to thoroughly characterize these complex chemical components, particularly in challenging environmental matrices. As questions surrounding microplastic exposure become increasingly important, researchers require analytical techniques capable of examining the chemistry of a sample in greater detail, not simply confirming the presence of the physical particle itself.</p></div><div><h3><strong>Thermal Desorption and Pyrolysis GCxGC-TOFMS For Deeper Microplastic Analysis</strong></h3><p><strong>Thermal Desorption</strong> (<strong>TD</strong>) and <strong>Pyrolysis </strong>(<strong>Py</strong>) coupled with comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://www.leco.com/core-analysis/gc-and-gc-ms/"><strong>GCxGC-TOFMS</strong></a>) offer a powerful alternative for investigating nano- and microplastics in complex samples. These techniques provide complementary information about sample composition.&nbsp;</p><p><strong>Thermal desorption</strong> releases volatile and adsorbed compounds, including environmental contaminants, degradation products, and VOCs associated with microplastic particles. This can reveal important information about chemicals present within or on the plastic.</p><p><strong>Pyrolysis </strong>thermally deconstructs the polymeric material, breaking it into GC-amenable characteristic degradation compounds. These compounds act as chemical markers that can be used to identify the various polymers present. When analyzing complex or heavily weathered microplastics that may be difficult to characterize using traditional spectroscopic techniques, pyrolysis is particularly advantageous.</p><p>The most significant benefit comes from pairing these techniques with comprehensive <strong>two-dimensional gas chromatography</strong>. Environmental samples often contain hundreds or even thousands of compounds that coelute in 1D GC-MS, making it difficult or impossible to identify each compound. GCxGC provides significantly greater separation capability, helping analysts distinguish important polymer markers from background matrix components. This enhanced separation improves the mass spectrometer’s ability to identify characteristic polymer pyrolysis products, even in highly complex environmental samples. As a result, researchers can detect and identify challenging microplastics without the extensive purification steps often required by other analytical workflows.</p><p>Another unique advantage of this approach is that thermal desorption and pyrolysis can be used in series on the same sample. Together, they create a more complete chemical profile of the sample.</p></div><div><h3><strong>A Powerful Microplastics Analysis Workflow for Deeper Insight into the Effects on Our Environment</strong></h3><p>FTIR and Raman spectroscopy remain valuable tools for rapid screening and flagging samples containing microplastic particles. However, by<strong> combining thermal desorption, pyrolysis, and GCxGC-TOFMS</strong>, analysts can obtain a <strong>much more comprehensive view of plastic</strong>s in their samples and their environmental implications. As environmental research continues to advance and concerns surrounding microplastic contamination grow, researchers will increasingly require information that extends beyond answering the question,&nbsp;<i>“What plastic is present?”&nbsp;</i>&nbsp;</p><p>Ultimately, this deeper level of characterization can provide new insights into the environmental fate of microplastics and help scientists better understand their potential impacts on ecosystems and human exposure.</p></div>

Surpassing the Limits of Traditional Microplastic Analysis Methods with Thermal Desorption and Pyrolysis GCxGC-TOFMS

Oligonucleotide and mRNA Analysis Resource Roundup

<p><strong>NIST26 introduces integrated chromatogram processing, deconvolution, and library searching for EI GC-MS and LC-MS/MS data, but the workflow begins with one essential requirement: chromatographic data must be available in the mzML format. In his presentation </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/Conversion_to_mz_ML_63e134bb34.pdf"><i><strong>Conversion to mzML Format for NIST26 Chromatogram Processing</strong></i></a><strong>, James Little of Mass Spec Interpretation Services explains how vendor data can be converted to mzML using either instrument-vendor software or the freely available MSConvert tool from ProteoWizard, and highlights several settings that are critical for successful processing in NIST26.</strong></p><h4><span><strong>NIST26 Chromatogram processing requires mzML</strong></span></h4><p>For the chromatogram-processing workflow described in the presentation, <strong>mzML is the accepted input format</strong>. Proprietary LC-MS or GC-MS data therefore need to be converted before they can be loaded into the NIST26 chromatogram-processing environment.</p><p>There are two principal ways to obtain an mzML file:</p><ul><li>conversion using software supplied by the instrument manufacturer,</li><li>conversion using <strong>MSConvert</strong>, which is included in the free <strong>ProteoWizard</strong> software package.</li></ul><p>Using an open format such as mzML also provides a practical way to move mass spectrometry data between different software environments without relying exclusively on proprietary vendor formats.</p><h4><span><strong>Using ProteoWizard and MSConvert</strong></span></h4><p>MSConvert is installed as part of the complete ProteoWizard package rather than as a separate application. After installing ProteoWizard, users can access either the graphical MSConvert interface or the command-line version.</p><p>The presentation recommends the stable release for routine work, while noting that newer or nightly builds may sometimes provide improved support for recently introduced vendor formats or additional control over data-processing filters. This can be particularly relevant when handling vendor files in which centroiding behavior or peak picking requires closer attention.</p><p>For most users, the graphical interface provides a straightforward route from proprietary raw data to an mzML file suitable for further NIST26 processing.</p><h4><span><strong>Start by selecting mzML as the output format</strong></span></h4><p>The first step in MSConvert is to select the source file or dataset using the <strong>Browse</strong> function.</p><p>The software supports a broad range of mass spectrometry data formats, and the file-selection window can either be restricted to a particular vendor format or set to display any supported spectral format.</p><p>After selecting the source data, the output format must be set to <strong>mzML</strong>. The screenshots in the presentation show this setting directly in the MSConvert options panel before the conversion is started.</p><p>The user can also select the destination folder and other file-output options before adding the selected dataset to the conversion queue.</p><h4><span><strong>Add the data and start the conversion</strong></span></h4><p>Once the source file and output settings have been selected, the file is added to the conversion list using the <strong>Add</strong> command.</p><p>The conversion itself is then launched with <strong>Start</strong>.</p><p>For simple datasets that are already stored in the correct spectral representation, this may be all that is required. However, James Little emphasizes an additional requirement that is particularly important for NIST26: the data must be <strong>centroided</strong>.</p><h4><span><strong>Centroided data are required</strong></span></h4><p>According to the presentation, <strong>profile-mode data are not acceptable for this NIST26 chromatogram-processing workflow</strong>. The spectra must instead be in centroided, or peak-picked, form.</p><p>Profile spectra contain the full measured shape of each mass spectral signal across the m/z axis. Centroided data reduce these profiles to discrete peaks represented by their characteristic m/z and intensity values.</p><p>If the original vendor data are still in profile mode, centroiding can be performed during conversion by adding the <strong>Peak Picking</strong> filter in MSConvert.</p><p>This means that the conversion step can perform two operations at once:</p><p><strong>vendor raw data → centroiding / peak picking → mzML</strong></p><p>The resulting mzML file can then be used as input for NIST26 chromatogram processing.</p><h4><span><strong>Peak Picking must be the first filter</strong></span></h4><p>One of the most important practical details in the presentation is the position of the Peak Picking operation in the MSConvert filter sequence.</p><p>If centroiding is required, <strong>Peak Picking must be the first algorithm or filter in the list</strong>. Placing another processing function before it can interfere with the intended conversion workflow.</p><p>MSConvert allows the user to select the Peak Picking filter, define the relevant MS levels, and add it to the processing sequence before starting conversion. The example shown in the presentation applies vendor-based peak picking to selected MS levels.</p><p>If there is uncertainty about whether the source data are already centroided, the Peak Picking filter can still be included. According to the presentation, applying it to data that are already centroided does not alter the file unnecessarily.</p><p>This provides a useful safeguard when the acquisition mode of an older dataset is uncertain.</p><h4><span><strong>Selecting the appropriate MS levels</strong></span></h4><p>Peak picking does not necessarily have to be applied identically to every spectrum in a dataset. MSConvert allows users to specify the <strong>MS levels</strong> to which the filter should be applied.</p><p>This is especially relevant for LC-MS/MS data, where a file can contain both precursor-level MS spectra and tandem MS spectra.</p><p>The conversion settings should therefore reflect the way the original data were acquired and how those data will subsequently be processed in NIST26.</p><h4><span><strong>Be aware of changes in file size</strong></span></h4><p>The presentation also notes that converted files may become substantially different in size from the original vendor files.</p><p>This is worth considering when working with large LC-MS/MS datasets or processing batches of chromatograms. The size of the resulting mzML file can depend on the original acquisition mode, centroiding, compression settings, the number of MS levels, and other output parameters.</p><p>Storage requirements should therefore be considered before converting large collections of raw data.</p><h4><span><strong>A simple workflow for preparing data for NIST26</strong></span></h4><p>The overall procedure presented by James Little can be summarized as a short sequence:</p><ol><li>Obtain the original vendor LC-MS or GC-MS data.</li><li>Open the data in MSConvert or use the vendor's own conversion software.</li><li>Select <strong>mzML</strong> as the output format.</li><li>Check whether the spectra are centroided.</li><li>If necessary, add <strong>Peak Picking</strong> as the first processing filter.</li><li>Select the appropriate MS levels.</li><li>Add the file to the conversion list.</li><li>Start the conversion.</li><li>Use the resulting mzML file for NIST26 chromatogram processing.</li></ol><p>The procedure itself is straightforward, but the centroiding requirement is important. A file can have the correct <code>.mzML</code> extension and still be unsuitable for the workflow if its spectra remain in profile mode.</p><h4><span><strong>From vendor data to integrated NIST26 processing</strong></span></h4><p>File conversion may appear to be a relatively minor step compared with deconvolution, spectral interpretation, and library searching, but it establishes the foundation for the entire downstream workflow.</p><p>By using mzML as the common input format, NIST26 can process data originating from different instrument platforms through a more consistent workflow. ProteoWizard and MSConvert provide a practical bridge between proprietary mass spectrometry files and this standardized format.</p><p>The key message from the presentation is therefore simple: <strong>convert to mzML, ensure the spectra are centroided, and place Peak Picking first whenever centroiding is required</strong>. Once those conditions are met, the data are ready for the integrated chromatogram-processing and library-searching capabilities available in NIST26.</p>

Conversion to mzML Format for NIST26 Chromatogram Processing

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In 1936, the Laboratory Equipment Company introduced the first rapid carbon determinator for the American iron and steel industry.  Today, almost 80 years later, LECO is recognized globally as a leader in the development of high-quality analytical instrumentation, mass spectrometers, comprehensive two-dimensional chromatography, metallography & optical equipment as well as consumables.

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My main interest is the identification of organic compounds by mass spectrometry in organic mixtures.

James Little/Mass Spec Interpretation Services

Galochrom Chiral ION-QD HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan.

<p><strong>Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QD-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Analytical Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION02-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>ION-QN</strong></a><strong> and ION-QD unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

<p><strong>Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QN-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Analytical Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 3</strong></li><li>Porosity: [Å] 200</li><li>Cat. No.: ION01-A150</li><li><strong>Price [€]: 1 300</strong></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral ION-QN and </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>ION-QD</strong></a><strong> unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Thermo Scientific Accucore RP-MS HPLC Columns

Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.

<p><strong>Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.</strong></p><p>Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations—without the elevated backpressures required by sub-2μm particles.</p><ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Optimized for MS detection</li><li>Excellent peak shapes</li><li>Excellent combination of speed and efficiency</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> Proprietary</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>USP Type: </strong>N/A</li><li><strong>Carbon Load</strong>: 7%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 9</li></ul>

Thermo Scientific Accucore C18 HPLC Columns

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

<p><strong>Achieve fast, high-resolution separations at low backpressures using Thermo Scientific™ Accucore™ C18 LC columns. Rugged 2.6μm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms.&nbsp;</strong></p><p>The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Optimal Selectivity for Nonpolar Compounds</strong></h5><ul><li>Hydrophobic interaction mechanism</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> C18</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L1</li><li><strong>Carbon Load</strong>: 9%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>1 to 11</li></ul>

Thermo Scientific Accucore C8 HPLC Columns

Improve separation speed and resolution while maintaining low backpressures using Thermo Scientific™ Accucore™ C8 LC Columns. 

<p><strong>Improve separation speed and resolution while maintaining low backpressures using Thermo Scientific™ Accucore™ C8 LC Columns. Rugged 2.6μm solid-core particles ensure high efficiencies and enable compatibility with both HPLC and UHPLC platforms.&nbsp;</strong></p><p>The high coverage C8 bonded phase provides optimal retention of analytes with moderate hydrophobicity. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Lower Hydrophobic Retention</strong></h5><ul><li>Recommended for moderately hydrophobic analytes or for when a less hydrophobic phase provides optimum retention</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Stationary Phase:</strong> C8</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L7</li><li><strong>Carbon Load</strong>: 5%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 9</li></ul>

Thermo Scientific Accucore aQ C18 Polar Endcapped HPLC Columns

Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns.

<p><strong>Separate polar compounds with improved speed and resolution using Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.</strong></p><p>The polar endcapped C18 stationary phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Alternative Selectivity</strong></h5><ul><li>Polar endcapped C18 stationary phase provides optimum retention of polar analytes</li><li>Accucore aQ Columns are compatible with highly aqueous mobile phases.</li><li>Polar encapping allows use in 100% aqueous mobile phases without loss of performance or stability.</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Polar</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> C18</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L1</li><li><strong>Carbon Load</strong>: 9%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 9</li></ul>

Thermo Scientific Accucore Polar Premium HPLC Columns

Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns.

<p><strong>Achieve fast, high-resolution separations of polar and nonpolar analytes using Thermo Scientific™ Accucore™ Polar Premium LC columns. Rugged 2.6μm solid-core particles enable fast, high-efficiency separations at low backpressures.&nbsp;</strong></p><p>The robust amide-embedded C18 stationary phase provides unique selectivity complementary to conventional C18 columns, is stable from pH 1.5–10, and is fully compatible with highly aqueous mobile phases. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.</p><h5><strong>Alternative Selectivity</strong></h5><ul><li>Polar embedded reverse phase material offers complementary selectivity to standard C18 columns.</li></ul><h5><strong>Stable over Wide pH Range</strong></h5><ul><li>Specially designed bonded phase is stable from pH 1.5–10.5.</li></ul><h5><strong>Compatible with Highly Aqueous Mobile Phases</strong></h5><ul><li>Rugged amide-embedded C18 phase will not undergo phase collapse in 100% aqueous mobile phase.</li></ul><h5><strong>Fast Separations</strong></h5><ul><li>2.6μm core-enhanced technology particles enable operation at high flow rates without generating excessive backpressures.</li></ul><h5><strong>Excellent Peak Shapes for Enhanced Resolution and Sensitivity</strong></h5><ul><li>Solid-core 2.6μm particles and narrow particle size distribution reduce band broadening and improve separation efficiencies.</li><li>High surface coverage of silica minimizes secondary interactions and peak tailing.</li></ul><h5><strong>Low Backpressures</strong></h5><ul><li>Solid-core 2.6μm particles for less backpressure compared to UHPLC sub-2μm particles</li><li>Compatible with conventional HPLC instrumentation and UHPLC platforms</li></ul><h5><strong>Excellent Reproducibility and Robustness</strong></h5><ul><li>Advanced bonding technology</li><li>Automated packing process</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> Polar Embedded</li><li><strong>Surface Area:</strong> 90 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L60</li><li><strong>Carbon Load</strong>: 9%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>150 Å</li><li><strong>pH: </strong>1.5 to 10.5</li></ul>

Thermo Scientific Accucore Phenyl-Hexyl HPLC Columns

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.

<p><strong>Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.</strong></p><p>Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.</p><ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes</li><li>Enhanced Pi-pi Interactions with Aromatics</li><li>Moderate Hydrophobicity</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>For Use With:</strong> HPLC</li><li><strong>Max. Pressure: </strong>14500 psi (1000 bar)</li><li><strong>Packing Material:</strong> Spherical, Solid Core, Ultrapure Silica</li><li><strong>Particle Shape:</strong> Spherical Solid Core</li><li><strong>Stationary Phase:</strong> Phenyl/Hexyl</li><li><strong>Surface Area:</strong> 130 m²/g</li><li><strong>Temperature:</strong> 70°C</li><li><strong>USP Type: </strong>L11</li><li><strong>Carbon Load</strong>: 5%</li><li><strong>Phase:</strong> Reversed Phase</li><li><strong>Pore Size: </strong>80 Å</li><li><strong>pH: </strong>2 to 8</li></ul>

Waters SEC Columns

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

<h4><strong>Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors</strong></h4><p>Get unmatched batch-to-batch reproducibility with the wide range of <strong>Waters Size-Exclusion Chromatography (SEC) Columns</strong> and standards for protein and peptide characterization.</p><p><strong>SEC</strong> is a powerful technique for aggregate and fragment analysis in the <strong>research, development</strong>, and <strong>manufacturing of biotherapeutic proteins</strong>, such as <strong>insulin</strong> and <strong>monoclonal antibodies</strong> (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.</p><p>The <strong>pore size and volume</strong> of the SEC particles used in the packed column drives this <strong>isocratic based technique</strong>, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.</p><h4><strong>Overview</strong></h4><ul><li>Gain efficiency with this “platform based” method for <strong>proteins ranging from 10,000 to 650,000 Daltons</strong>, leveraging <strong>MaxPeak Premier Technology</strong> coupled with <strong>BEH-PEO Particle Technology&nbsp;</strong> &nbsp;&nbsp;</li><li><strong>Minimize non-desired secondary interactions </strong>for improved separation reproducibility and results with Ethylene Bridged Hybrid (BEH) SEC particles</li><li>Ensure reliable <strong>column-to-column and batch-to-batch performance</strong> with columns quality control tested with relevant peptide, protein, and mAb samples &nbsp;</li><li>Achieve reliable <strong>high throughput analysis on UPLC</strong> systems with our <strong>1.7 µm particle size</strong> offering and reproducible separations on any <strong>HPLC, UHPLC, or UPLC system</strong> to address different application needs with <strong>2.5 µm&nbsp;</strong></li><li><strong>Extend analytical column life</strong> and<strong> lower cost per analyses </strong>with SEC Guards&nbsp;</li><li>Confirm on-site SEC column and LC system performance with available BEH125, BEH200, BEH450, and mAb Size Variant Standards &nbsp;&nbsp;</li><li><strong>Manufactured in a cGMP, ISO 9001</strong> certified plant using stringent manufacturing protocols and ultra-pure reagents</li></ul><p><strong>Recommended Use: </strong>For size-based peptide, protein, and viral vector analyses.</p><h4><strong>System Dispersion Specifications for Column Recommendations</strong></h4><h5><strong>LC System Dispersions &lt;20 µL (UPLC)</strong></h5><ul><li>ACQUITY Premier Protein SEC 250 Å Guards and Columns</li><li>ACQUITY UPLC Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>1.7 µm guards and columns for low dispersion UPLC systems</li></ul><h5><strong>LC System Dispersions &gt;20 µL – ≤35 µL (UHPLC)</strong></h5><ul><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>2.5 µm guards and columns for UHPLC systems</li></ul><h5><strong>LC System Dispersions &gt;35 µL (HPLC)</strong></h5><ul><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>BioSuite SEC Guards and Columns</li><li>3.5 µm guards and columns for HPLC systems</li></ul><h4><strong>GTxResolve SEC Columns</strong></h4><h5><strong>GTxResolve Premier BEH SEC 450 Å Columns</strong></h5><ul><li>2.5 µm particle size columns for UHPLC and UPLC separations</li><li>Lower secondary interactions and lower noise suitable for platform methods</li><li>Low adsorption hardware necessary for analysis of biologics samples</li><li>450 Å pore size with up to 50% higher resolving power reducing need for sample consumption, resulting in faster analyses<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><h5><strong>GTxResolve Premier SEC 1000 Å Columns</strong></h5><ul><li>3 µm columns for UHPLC and UPLC separations</li><li>1000 Å columns with high efficiency for aggregate analysis of GTx drugs with diameter of 80-200 nm</li><li>Low secondary interaction &amp; improved compatibility with universally applied detector, such as MALS</li><li>Low adsorption hardware necessary for analysis of biologics samples<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg" alt="Waters: GTxResolve SEC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Protein SEC Columns</strong></h4><h5><strong>ACQUITY Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations</li><li>250 Å pore size</li><li>High throughput &amp; resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-premier-columns.html"><strong>Explore ACQUITY Premier Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg" alt="Waters: ACQUITY Premier Columns featuring MaxPeak HPS Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg 234w," sizes="100vw" width="1024" height="682"></p><h5><strong>XBridge Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for HPLC and UHPLC separations</li><li>250 Å pore size</li><li>High resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on any LC system</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology</li><li>Specifically designed and QC tested with NIST-based, mAb Size Variant Standard to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Waters-XBridge-Premier-Columns/nav.htm"><strong>Explore XBridge Premier Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Premier_Columns_0230c90278.jpg" alt="Waters: XBridge Premier Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Premier_Columns_0230c90278.jpg 193w," sizes="100vw" width="950" height="768"></p><h5><strong>BioResolve mAb SEC Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for UPLC, UHPLC, and HPLC separations</li><li>200 Å pore size</li><li>Obtain reproducible, accurate quantitation of monoclonal antibodies (mAbs), associated high molecular weight aggregates (&gt;300,000 Da), and lower molecular weight fragments (e.g., &lt;100,000 Da)</li><li>Each packed column is tested with NIST-based, mAb size variant standard to help ensure batch-to-batch and column-to-column performance consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioResolve-SEC-mAb-Consumables-%7C-SEC-Guards/nav.htm"><strong>Explore BioResolve mAb SEC Guards and Columns</strong></a></li></ul></li></ul><h5><strong>ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations (2.5 μm for 450 Å)</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency</li><li>10x faster than traditional HPLC-based SEC<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-uplc-columns.html"><strong>Explore ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Acquity_UPLC_kolony_1dc5c25d3e/Waters-Acquity-UPLC-kolony.jpg" alt="Waters: Acquity UPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Acquity_UPLC_kolony_1dc5c25d3e/thumbnail_Waters-Acquity-UPLC-kolony.jpg 245w," sizes="100vw" width="807" height="407"></p><h5><strong>XBridge Protein BEH SEC Columns</strong></h5><ul><li>2.5 μm particle columns for use on any UPLC, UHPLC, or HPLC system</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/XBridge-BEH-Columns-for-High-pH-Chromatography/nav.htm"><strong>Explore XBridge Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Columns_b0b55de501/Waters-XBridge-Columns.jpg" alt="Waters: XBridge Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Columns_b0b55de501/thumbnail_Waters-XBridge-Columns.jpg 203w," sizes="100vw" width="900" height="691"></p><h4><strong>Peptide SEC Columns</strong></h4><h5><strong>BioSuite SEC Columns</strong></h5><ul><li>Silica-based diol SEC columns</li><li>Molecular weight exclusion limits are determined primarily by the selected pore size</li><li>Available in analytical and semi-preparative column sizes<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioSuite-Columns/nav.htm"><strong>Explore BioSuite SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Suite_Columns_bf2bb8249f.jpg" alt="Waters: BioSuite Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Suite_Columns_bf2bb8249f.jpg 184w," sizes="100vw" width="307" height="260"></p><h5><strong>Protein-Pak SEC Columns</strong></h5><ul><li>Based on a 10 μm diol-bonded silica</li><li>Resolve proteins that differ in molecular weight by a factor of two</li><li>Distinguish proteins differing by as little as 15% in molecular weight<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Protein-Pak-HPLC-and-UPLC-Columns/nav.htm"><strong>Explore Protein-Pak SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Protein_Pak_Columns_be6a669326.jpg" alt="Waters: Protein-Pak Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Protein_Pak_Columns_be6a669326.jpg 245w," sizes="100vw" width="500" height="315"></p><h4><strong>Choose the right SEC Column for your lab</strong></h4><p>SEC is ideal for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic peptides, proteins, and viral vectors, but choosing the right column for your analysis is important. Use the following criteria to choose a column that provides reliable, high quality SEC analyses of various biomolecules, including insulin, monoclonal antibodies, and adeno associated viruses (AAVs).</p><h5><strong>What to consider when choosing an SEC Column:</strong></h5><ul><li>What is the molecular weight of your sample?</li><li>What is the dispersion of your LC system?</li><li>Are you separating components &lt;2 fold difference in molecular weight (MW)?</li><li>Are you separating components &gt;2 fold difference in MW?</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg" alt="Waters: Choose the right Size-exclusion chromatography (SEC) column for your analysis with Waters SEC columns selection guide." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg 158w," sizes="100vw" width="1024" height="1013"></p><h4><strong>Addressing the complexity of SEC Separations</strong></h4><p>Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards.</p><h5><strong>With MaxPeak Premier SEC Columns, you can:</strong></h5><ul><li>Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications</li><li>Use a ‘platform SEC method’ to reduce method development time and cost</li><li>Ensure batch-to-batch and column-to-column performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg" alt="Waters: Classes of therapeutic proteins separated on an ACQUITY Premier Protein SEC Column." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters Atlantis Premier BEH Z-HILIC Column

Waters Atlantis Premier BEH Z-HILIC Columns provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development.

<p><strong>Atlantis Premier BEH Z-HILIC Columns </strong>provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development. &nbsp;Built using rugged <strong>ethylene bridged hybrid particles</strong>, these columns are <strong>pH stable from pH 2-10</strong>; the MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Premier_BEH_Z_HILIC_kolony_1_59d95946d7/Waters-Atlantis-Premier-BEH-Z-HILIC-kolony-1.jpg" alt="Waters: Atlantis Premier BEH Z-HILIC column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Premier_BEH_Z_HILIC_kolony_1_59d95946d7/thumbnail_Waters-Atlantis-Premier-BEH-Z-HILIC-kolony-1.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specification</strong></h4><ul><li><strong>Chemistry:</strong> Sulfobetaine</li><li><strong>Separation Mode: </strong>Hydrophilic Interaction (HILIC)</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max:</strong> 10 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>No</li><li><strong>Bonding Technology:</strong> Zwitterion</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Particle Size:</strong> 1.7 µm</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>Pore Size: </strong>95 Å</li><li><strong>Format: </strong>Premier Column</li><li><strong>Surface Area:</strong> 270</li><li><strong>System:</strong> UHPLC, UPLC</li><li><strong>Particle Technology: </strong>BEH</li><li><strong>USP Classification:</strong> L122</li><li><strong>Carbon Load: </strong>17 %</li></ul><h3><strong>Atlantis Premier Columns</strong></h3><h4><strong>Next-generation hybrid particle and surface technology for increased polar analyte retention</strong></h4><p>Retaining polar compounds is one of the most challenging chromatographic problems. With the hybrid particle technology in Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns, those problems are a thing of the past. These reversed-phase, anion-exchanged, mixed-mode, and zwitterionic columns are the next evolution of columns in the Atlantis family, all designed to achieve superior polar compound retention.</p><p>Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns combine a new and innovative chromatographic particle technology with proprietary MaxPeak High-Performance Surfaces (HPS) Technology to boost retention of polar acidic compounds by 50% while eliminating unwanted analyte-surface interactions.</p><h4><strong>Atlantis Premier Column Characteristics</strong></h4><h5><strong>Atlantis Premier BEH 95 Å Particle Columns</strong></h5><ul><li>Mixed-mode C18 Anion-exchange (AX) Chemistry</li><li>Increase retention of polar acids</li><li>Wider pH range (2–10)</li><li>Batch-to-batch reproducibility</li><li>Mass Spectrometry (MS) compatible</li></ul><h5><strong>Atlantis Premier BEH Z-HILIC Columns</strong></h5><ul><li>Uses sulfobetaine zwitterionic ligand</li><li>Increase retention of strongly polar analytes</li><li>Wider pH range (2–10)</li><li>Batch-to-batch reproducibility</li><li>Mass Spectrometry (MS) compatible</li></ul>

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