<p><strong>Whether a drug can effectively bind to its target protein, or how different parts of a virus or a cell interact, depends on processes taking place at the level of individual molecules. Their behaviour is determined not only by their shape, but also by the electrostatic forces acting between them. Understanding how these forces are distributed helps scientists explain why certain molecules attract each other, how they bind and how they interact.</strong></p><p>This is where the <a target="_blank" rel="noopener noreferrer" href="https://acc.biodata.ceitec.cz/"><i><strong>Atomic Charge Calculator (ACC)</strong></i></a> comes in. Researchers at Masaryk University have been developing this computational tool for more than a decade. Its third and most advanced generation to date was developed at <strong>CEITEC Masaryk University</strong> and the<strong> Faculty of Science at Masaryk University</strong>, in collaboration with the <strong>Faculty of Informatics at Masaryk University</strong> and <strong>Palacký University Olomouc</strong>.</p><p><strong>ACC III </strong>calculates the electric charges of individual atoms and visualises their distribution directly within three-dimensional molecular structures. It brings together <strong>twenty different calculation methods</strong> in a single environment and can work with a wide range of molecules – from small organic compounds to proteins and other large biomolecular structures.</p><p>A key advantage of <strong>Atomic Charge Calculator III</strong> is that it combines a broad range of computational methods with ease of access. Basic calculations can be performed directly in a web browser, <strong>with no need to install specialised software or register for an account</strong>. The resulting charges can be displayed directly on a 3D model of the molecule. For advanced users and large-scale analyses, the platform also supports automated data processing and allows its computational core to be run locally.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Co_pritahuje_molekuly_Nova_generace_nastroje_z_CEITEC_zviditelnuje_sily_mezi_atomy_d32d7bd2cd.jpg" alt="CEITEC/Biological Data Management and Analysis Core Facility: What Attracts Molecules? A New Generation of a CEITEC Tool Makes the Forces Between Atoms Visible: Oseltamivir bound to influenza virus neuraminidase (blue: positive partial charges, red: negative charges). A strong electrostatic interaction occurs between a negatively charged region of the drug and positively charged regions of the protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Co_pritahuje_molekuly_Nova_generace_nastroje_z_CEITEC_zviditelnuje_sily_mezi_atomy_d32d7bd2cd.jpg 140w," sizes="100vw" width="1600" height="1786"></p><h3><strong>Why are scientists interested in the electric charges of atoms?</strong></h3><p>Electrons are not distributed evenly within molecules. Individual atoms can therefore carry different partial electric charges. These charges play an important role in determining how molecules behave towards their surroundings and towards one another.</p><p>Positively and negatively charged regions of molecules can attract each other, while regions with the same charge repel each other. The distribution of these charges therefore helps determine which molecules approach each other, how they orient themselves and how strongly they may bind. In biology, this is important, for example, in molecular recognition or when a drug binds to its target protein. Understanding partial charges therefore helps scientists gain insight into the interactions underlying the behaviour of many molecular systems.</p><p>There is, however, no single universal way to determine partial charges. Different computational methods can produce different values, and the choice of method also depends on how the results will be used. Accurate quantum-chemical calculations can also become very computationally demanding when large numbers of structures are involved. ACC III therefore brings together<strong> empirical methods </strong>that allow these calculations to be performed more quickly and on a large scale.</p><p><i>“From the outset, we designed ACC III to make calculating partial atomic charges as straightforward as possible. Users do not need to install anything or deal with complicated settings – they simply upload a molecule and start the calculation with a single click. The application then suggests suitable methods from the twenty available and can handle both small molecules and large biomolecular structures,”</i> says <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/tomas-racek-ph-d/u94476?page=publication"><strong>Tomáš Raček</strong></a>, who has been developing and maintaining ACC since 2018.</p><h3><strong>How an antiviral drug binds to an influenza virus protein</strong></h3><p>The authors demonstrate what charge distribution can reveal using the well-known antiviral drug oseltamivir, marketed as Tamiflu. The drug binds to neuraminidase, a protein of the influenza A virus. ACC III can show directly within the three-dimensional structure where positive and negative partial charges are located and how they contribute to the interaction between the drug and the protein.</p><p><i>“The example of oseltamivir clearly shows why it is useful not only to calculate the charges, but also to visualise them directly within the structure. This allows us to see which parts of the two molecules interact electrostatically. The same approach can be used to study many other molecular systems,”</i> explains Tomáš Raček.</p><p><strong>ACC III is not limited to small molecules</strong>. It can also work with proteins and other large biomolecular structures. The authors demonstrate this using a second example: the <strong>SARS-CoV-2 </strong>pore complex. Coronaviruses form these pores during replication in infected cells and use them to transport viral RNA across membranes. Visualising partial charges helps distinguish the parts of the complex that pass through the non-polar environment of the membrane from its more highly charged regions.</p><h3><strong>From calculation straight to a 3D model</strong></h3><p>The new generation of the Atomic Charge Calculator also offers<strong> more advanced visualisation</strong>. It uses Mol*, which allows calculated charges to be mapped directly onto a three-dimensional structure and explored interactively. A visualisation created using ACC III was also selected for the<strong> cover of the special </strong><i><strong>Nucleic Acids Research</strong></i><strong> Web Server Issue</strong>. Created by Tomáš Raček, the cover image shows the distribution of partial charges in a membrane protein system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Co_pritahuje_molekuly_Nova_generace_nastroje_z_CEITEC_zviditelnuje_sily_mezi_atomy_3_85cc310925.jpeg" alt="CEITEC/Oxford University Press: What Attracts Molecules? A New Generation of a CEITEC Tool Makes the Forces Between Atoms Visible: Cover page in Nucleic Acids Research journal." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Co_pritahuje_molekuly_Nova_generace_nastroje_z_CEITEC_zviditelnuje_sily_mezi_atomy_3_85cc310925.jpeg 124w," sizes="100vw" width="1600" height="2011"></p><p>Results can be downloaded in several standard formats and, for the first time, stored directly in the mmCIF format commonly used for three-dimensional biomolecular structures. Information about the structure and its calculated charges can therefore remain together in a single file and be used by other tools.</p><p>For researchers who need to process large numbers of structures, ACC III also provides an API and a computational core that can be run locally. The platform can therefore be used both for individual calculations through its web interface and as part of larger automated analyses.</p><h3><strong>More than a decade of development</strong></h3><p>Scientists introduced the first Atomic Charge Calculator in <strong>2015</strong>, followed by its second generation in <strong>2020</strong>. In the meantime, the team also developed specialised tools: PDBCharges for structures from the Protein Data Bank and AlphaCharges for structures from the AlphaFold Database.</p><p><i>“The Atomic Charge Calculator was never a one-off project,”</i> says <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/prof-radka-svobodova-ph-d/u17518?page=publication"><strong>Radka Svobodová</strong></a>, Head of the Biological Data Management and Analysis Core Facility at CEITEC Masaryk University. <i>“For more than a decade, we have been continuously developing tools that respond both to new possibilities in structural bioinformatics and to the needs of their users. The third generation combines straightforward web-based use with capabilities that are important for the automated processing of large numbers of molecular structures.”</i></p><blockquote><p><strong>Atomic Charge Calculator III is freely available through its web interface and does not require registration for basic use.</strong> Its development was supported by the<strong> ELIXIR CZ </strong>research infrastructure.</p></blockquote><h5><strong>The authors presented the new generation of the tool in </strong><a target="_blank" rel="noopener noreferrer" href="https://academic.oup.com/nar/article/54/W1/W211/8662890"><i><strong>Nucleic Acids Research</strong></i></a><strong>.</strong></h5>

What Attracts Molecules? A New Generation of a CEITEC Tool Makes the Forces Between Atoms Visible 

<p><span><strong>Not every concentration job can tolerate heat — and not every concentrator that handles heat well can handle cold. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2441"><span><strong>Refrigerated centrifugal vacuum concentrators</strong></span></a><span><strong> exist for a specific reason: proteins, RNA, enzymes, and synthetic peptides degrade quickly under thermal stress, and standard concentrators that rely on heat alone can compromise sample integrity before evaporation finishes. We compared two of the leading refrigerated systems — the Labconco Refrigerated CentriVap and the </strong></span><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2611"><span><strong>Thermo Fisher Refrigerated SpeedVac SRF110</strong></span></a><span><strong> — to see how they stack up for labs where sample preservation is non-negotiable.</strong></span></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/labconco-refrigerated-centrivap-vs-thermo-fisher-refrigerated-speedvac"><span><strong>Download the full comparison with pricing here!</strong></span></a></p></blockquote><h3><strong>The Problem Refrigeration Actually Solves</strong></h3><p>It's a detail that gets lost in most spec-sheet comparisons: running a<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?manufacturerItems=540&amp;search=centrivap"><strong> vacuum concentrator </strong></a>without temperature control can cause a sample to over-cool itself. As solvent evaporates under vacuum, the process pulls latent heat from the sample — the same evaporative cooling effect that makes sweat feel cold on skin. For a heat-stable solvent, that's irrelevant. For a temperature-sensitive biomolecule, it can drop the sample low enough to risk unwanted freezing or sublimation mid-run. Labconco's own engineering notes for the Refrigerated CentriVap describe this directly: the unit pairs a refrigeration system with a heater so operators can hold a precise set-point temperature and avoid sublimated samples caused by evaporative cooling. That's the whole reason this product category exists, and it's worth understanding before comparing specs line by line.</p><h3><strong>Price: A Wider Gap at the High End</strong></h3><p>The <strong>Labconco Refrigerated CentriVap</strong> is typically priced around <strong>$13,000 USD</strong>. The <strong>Thermo Fisher SRF110</strong> runs higher, generally<strong> $15,000–$17,000 USD</strong>, and that's before factoring in the modular kit components — Thermo Fisher sells the SRF110 with a separate vacuum system, rotor, vapor trap, and heat transfer fluid bundled into the total kit price. The price gap between these two refrigerated units is proportionally larger than what we found comparing their non-refrigerated counterparts, which makes the value case for the CentriVap even more pronounced at this tier.</p><h3><strong>Capacity: The Difference That Matters Most Right Now</strong></h3><p>This is where the two systems really separate, and it lines up with where lab demand is heading. The Refrigerated CentriVap processes up to <strong>312 samples</strong> per run; the SRF110 tops out at <strong>132</strong>. Both handle volumes up to 50 mL, so this isn't a volume trade-off the way it was in our earlier comparison — it's a straight throughput advantage for the CentriVap.</p><p>That gap matters because<a target="_blank" rel="noopener noreferrer" href="https://peptidestaff.com/industry-trends/peptide-therapy-market-forecast-2026/"><strong> peptide-based therapeutics </strong></a>have become one of the fastest-growing classes of drugs in the pharmaceutical industry, and manufacturing capacity — not demand — is widely cited as the primary bottleneck holding the sector back. <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7541"><strong>GLP-1 programs</strong></a> in particular are pushing <a target="_blank" rel="noopener noreferrer" href="https://www.bachem.com/knowledge-center/glp-1-demand-for-peptide-manufacturers/"><span><strong>peptide manufacturers</strong></span></a><span> </span>toward industrialized, large-scale production platforms rather than one-off batch work. <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/"><strong>Sample prep equipment</strong></a> sits squarely inside that bottleneck: every purification run, HPLC fraction, and concentration step has to scale alongside the chemistry. A concentrator that processes more than double the samples per run, like the Refrigerated CentriVap, is a meaningfully different proposition for a peptide synthesis or purification lab than it would have been five years ago.</p><h3><strong>Temperature Range: How Cold You Can Go</strong></h3><p>The CentriVap's refrigeration reaches as low as<strong> -10°C</strong>; the SRF110 bottoms out at <strong>-4°C</strong>. Both units heat up to +100°C. That six-degree gap on the cold end isn't dramatic on paper, but for the most heat-labile peptides and enzymes, the lower floor gives operators more margin before a sample is at risk — particularly during longer runs where evaporative cooling has more time to compound any thermal load on the sample.</p><h3><strong>Vortex Speed and Mechanical Flexibility</strong></h3><p>The CentriVap offers an adjustable vortex range from <strong>500 to 1,800 rpm</strong>, while the SRF110 runs up to<strong> 1,725 rpm</strong>. The CentriVap's lower floor is the more useful distinction here — fragile, large, or shear-sensitive samples (think intact proteins or larger peptide constructs) sometimes need to run at reduced rotor speed to avoid mechanical stress, and the CentriVap gives operators that headroom on both ends rather than just a ceiling.</p><h3><strong>Lid Material: Visibility vs. Durability</strong></h3><p>The Refrigerated CentriVap uses a <strong>glass lid</strong>, letting operators see into the chamber during a run. The SRF110 uses an <strong>epoxy-coated aluminum lid</strong>, which trades visibility for added durability. For labs running visually distinct sample batches — colored buffers, multiple project codes, HPLC fractions pulled at different time points — the ability to glance at the chamber without interrupting a run has real workflow value, especially in a core facility setting where one instrument serves multiple research groups.</p><h3><strong>What This Means for Core Facilities</strong></h3><p>Shared instrumentation cores feel throughput and cold-temperature constraints differently than a single-PI lab does. A<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/an-appreciation-for-academic-core-labs"><strong> core running samples from multiple research groups </strong></a>— some doing routine RNA cleanup, others running heat-labile peptide fractions from an HPLC purification step — needs an instrument that won't bottleneck on either axis. The CentriVap's combination of higher per-run capacity and a lower refrigeration floor gives a shared facility more room to serve mixed workloads without forcing every group onto the same conservative settings a less flexible unit would require.</p><h3><strong>Built for the Same Footprint</strong></h3><p>Both units are sized for fume hood placement, with the CentriVap measuring<strong> 15.0 x 27.2 x 13.9 inches </strong>against the SRF110's<strong> 16.62 x 25 x 13.75 inches </strong>— close enough that floor planning isn't a deciding factor either way. Both carry <strong>standard one-year warranties</strong>.</p><h3><strong>The Bottom Line</strong></h3><p>For labs concentrating proteins, RNA, enzymes, or peptide fractions at scale — particularly anyone supporting peptide therapeutic development, where production capacity is already the limiting factor industry-wide — the Labconco Refrigerated CentriVap's combination of higher throughput, lower cold-temperature floor, and lower price makes it the stronger fit. The SRF110 remains a capable option for labs with smaller batch sizes already standardized on Thermo Fisher's modular vacuum ecosystem.</p><p>Organomation distributes the full Labconco CentriVap line, including the Refrigerated and Refrigerated Pro models. Contact our team at sales@organomation.com to talk through rotor configuration, cold trap pairing, and throughput planning for your lab.&nbsp;</p><blockquote><h5><a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/labconco-refrigerated-centrivap-vs-thermo-fisher-refrigerated-speedvac"><span><strong>Download the full comparison with pricing here!</strong></span></a></h5></blockquote>

Refrigerated SpeedVac SRF110 vs. CentriVap - Why Sample Capacity Matters More Than Ever for Peptide and Protein Labs

<p><strong>What if you could assess a tumor's metastatic potential — without extensive sample prep — directly from tissue? Rachel Wood is working on exactly that.</strong></p><p><strong>Rachel Wood</strong> is a PhD candidate at Queen's University (Kingston, Ontario),&nbsp;<br>working jointly in the labs of Dr. Richard Oleschuk (Chemistry) and Dr. Chris&nbsp;<br>Nicoll (Pathology &amp; Molecular Medicine). Her research applies Ambient Ionization Mass Spectrometry — specifically the Liquid Microjunction Surface Sampling Probe &nbsp;(LMJ-SSP) and Desorption Electrospray Ionization (DESI) — to profile the metabolomics of non-metastatic and metastatic clear cell Renal Cell Carcinoma (ccRCC) cell lines. The long-term goal: a rapid, minimally invasive MS-based prognostic tool for kidney cancer.</p><p>This is the first reported use of LMJ-SSP to assess metastatic status in ccRCC —&nbsp;<br>and early PCA results are already showing distinct separation between cell line&nbsp;<br>profiles.</p><h5><strong>In this episode, David and Rachel cover:</strong></h5><ul><li>Why <strong>ccRCC</strong> is such a resistant biomarker problem despite active genomic, proteomic, and metabolomic research</li><li>The clinical stakes of "<strong>diagnosed by accident</strong>" — and what a prognostic biomarker actually needs to do for a clinician</li><li>How AIMS complements (not replaces) LC-MS in a discovery-phase cancer workflow</li><li><strong>LMJ-SSP vs. DESI</strong>: what each technique sees that the other misses, including spatial resolution differences (~1mm vs. ~50µm)</li><li>Why Rachel runs LMJ-SSP on both a triple quad and a Q-TOF — and what each instrument contributes to the same research question</li><li>How her lab repurposed a 3D printer as a custom automated XYZ sampling stage with 50µm movement precision</li><li>Managing high-dimensional metabolomics data across two polarities, two cell lines, and two mass analyzers — the pipeline explained</li><li>What unsupervised machine learning (PCA) showed in early results</li><li>The path from cell lines to excised tumor tissue to, ultimately, liquid biopsies</li><li>Advice for analytical chemists considering work at the chemistry–oncology interface</li></ul><h3><strong>Video Transcription</strong></h3><p><strong>Kidney cancer remains a difficult biomarker problem, particularly when researchers try to distinguish tumors that are likely to behave aggressively from those that are not. In a recent episode of </strong><i><strong>Concentrating on Chromatography</strong></i><strong>, PhD researcher Rachel Wood discussed how she is combining rapid ambient ionization mass spectrometry, LMJ-SSP, DESI, multivariate data analysis, and conventional LC-MS workflows to investigate the molecular differences associated with clear cell renal cell carcinoma and its metastatic potential.</strong></p><h4><strong>Returning to the research question that started it all</strong></h4><p>Wood first encountered kidney cancer research during her undergraduate studies. Although she later moved into a more instrumentation-focused master’s project involving breast cancer, the subject of kidney cancer remained unfinished business.</p><p>When the opportunity arose to choose a PhD project, she returned to the field that had originally sparked her interest in research. She is now conducting her doctoral work at <strong>Queen’s University in Kingston, Ontario</strong>, in an interdisciplinary environment spanning chemistry, pathology, molecular medicine, computer science, and clinical research.</p><h4><strong>Why clear cell renal cell carcinoma is such a difficult biomarker problem</strong></h4><p>Wood’s research focuses on <strong>clear cell renal cell carcinoma (ccRCC)</strong>, which represents the majority of kidney cancer cases discussed in the interview.</p><p>The difficulty is not a lack of research effort. Genomics, proteomics, and metabolomics studies have all produced promising biomarker candidates. The challenge is that different studies often identify different sets of markers, with relatively little overlap between them.</p><p>Patient-to-patient biological variability further complicates the picture. A candidate biomarker panel that performs well in one cohort may not reproduce equally well in another. Selecting which features deserve further investigation therefore becomes a major analytical and biological challenge.</p><h4><strong>From incidental diagnosis to prognostic information</strong></h4><p>Another difficulty is the way kidney cancer is often discovered.</p><p>Rather than being detected through a dedicated screening program, tumors may be found incidentally during imaging performed for another reason. According to Wood, the ideal solution would be a diagnostic biomarker capable of detecting kidney cancer early. Such a marker is not currently available within the scope of the work she described.</p><p>That places additional importance on <strong>prognostic biomarkers</strong>. Once a tumor has been detected, clinicians need as much information as possible about how aggressive it may be and how likely it is to progress.</p><p>Wood’s current work is therefore not attempting to declare directly that a particular patient has metastatic disease. Instead, the goal is to understand <strong>what changes biologically as a tumor moves from a less aggressive to a more aggressive state</strong>.</p><p>That means asking which molecular pathways change, which mechanisms become altered, and which analytes may eventually provide useful indicators of tumor behavior.</p><h4><strong>AIMS and LC-MS answer different questions</strong></h4><p>A central theme of the interview was the relationship between rapid ambient ionization mass spectrometry and conventional LC-MS.</p><p>Wood is clear that <strong>AIMS is not intended to replace LC-MS</strong>. Instead, she sees the two approaches as complementary.</p><p>AIMS provides a rapid way to obtain a broad molecular overview of a sample with minimal preparation. This makes it especially useful during the discovery phase, when the goal is to gather as much molecular information as possible and identify features that distinguish biological groups.</p><p>LC-MS then becomes valuable when those features need to be separated, characterized more carefully, and quantified with greater confidence.</p><p>In Wood’s workflow, rapid MS screening can therefore help identify which analytes deserve closer attention before more targeted LC-MS investigations are performed.</p><h4><strong>What “minimal sample preparation” actually means</strong></h4><p>The amount of sample preparation required depends strongly on the type of material being analyzed.</p><p>Wood currently works mainly with <strong>cultured human cell lines</strong>, which means much of the preparation begins with growing the cells themselves. Depending on the experiment, the cells may then be chemically lysed or subjected to fixation, paraffin embedding, and sectioning before analysis by <strong>LMJ-SSP</strong> or <strong>DESI</strong>.</p><p>She also notes that the possibilities would be different for freshly excised tumor material. In an appropriate perioperative or biosafety laboratory setting, LMJ-SSP could potentially be used to analyze tumor margins more directly.</p><p>The practical meaning of “minimal preparation” is therefore highly dependent on the sample and the analytical question being asked.</p><h4><strong>Why combine LMJ-SSP and DESI?</strong></h4><p>Rather than choosing between LMJ-SSP and DESI, Wood uses the two approaches because they provide different types of information.</p><p><strong>LMJ-SSP</strong> offers rapid, localized analysis and can accommodate samples with uneven surface topography, which is particularly useful for excised tissue.</p><p><strong>DESI</strong>, by contrast, provides substantially higher spatial resolution. In the workflow described in the interview, DESI can achieve spatial resolution on the order of tens of micrometers, whereas LMJ-SSP operates at a much larger sampling scale depending on probe design.</p><p>That higher spatial resolution is important when moving from simplified cell-line models toward actual tumor tissue, where the molecular organization of different regions may reveal biologically relevant differences.</p><p>Combining both techniques therefore enables researchers to balance speed, surface compatibility, and spatial information.</p><h4><strong>Two mass analyzers for two parts of the same problem</strong></h4><p>Wood also uses two different mass spectrometers in her LMJ-SSP work.</p><p>Most of the discovery work is carried out using a <strong>triple quadrupole mass spectrometer</strong>, particularly because her project focuses strongly on lower-mass analytes and small molecules.</p><p>Once statistically important analytes have been identified, a <strong>Q-TOF</strong> instrument can be used for MS/MS experiments and tentative structural identification.</p><p>The two instruments therefore address different stages of the workflow: one supports broad discovery and comparison, while the other provides additional structural information needed to connect selected features with possible metabolic pathways.</p><h4><strong>Turning a 3D printer into an automated sampling platform</strong></h4><p>One of the most unusual elements of the project is the automated sampling system used with LMJ-SSP.</p><p>Historically, LMJ-SSP measurements could be performed manually or with custom-built XYZ positioning stages. Wood’s laboratory instead adapted a <strong>3D printer</strong> into an automated positioning system.</p><p>Custom software controls the printer and moves the sampling probe with precise control over its X, Y, and Z positions. According to Wood, the system enables movements down to approximately <strong>50 µm</strong> and also controls parameters such as the contact time between the probe and sample and the delay between consecutive sampling events.</p><p>This level of automation improves reproducibility because variables that would otherwise depend on manual operation can be controlled systematically.</p><p>Even the Z-position can be programmed, allowing the probe to operate at constant or varying heights depending on the sample surface.</p><h4><strong>Managing high-dimensional MS data</strong></h4><p>The experimental design creates a large data-analysis challenge.</p><p>Wood is working with multiple cell lines, both ionization polarities, and more than one mass analyzer. To maintain traceability, she analyzes the data in clearly defined subsets.</p><p>A typical workflow might begin with one mass analyzer and one polarity. Within that condition, the lower-aggressiveness and higher-aggressiveness cell models can then be compared directly.</p><p>The data processing pipeline includes steps such as:</p><ul><li>binning,</li><li>normalization,</li><li>transformation,</li><li>dimensionality reduction,</li><li>identification of discriminating molecular features.</li></ul><p>Once relevant features are identified, MS/MS analysis can be used to investigate them further. The same process is then repeated across other polarities and instruments.</p><h4><strong>Unsupervised machine learning reveals distinct molecular profiles</strong></h4><p>Machine learning is particularly valuable because the AIMS datasets are highly dimensional, containing very large numbers of molecular features across multiple samples.</p><p>Wood’s initial analysis has included <strong>principal component analysis (PCA)</strong>, an unsupervised dimensionality-reduction technique.</p><p>The early results are encouraging: the non-metastatic and metastatic cell-line models separate clearly in PCA space.</p><p>That does not itself identify a definitive biomarker panel, but it indicates that the two biological states have distinguishable molecular profiles even though both belong to the same kidney cancer subtype.</p><p>This provides an important foundation for identifying which specific metabolites or molecular pathways may be responsible for the separation.</p><h4><strong>Testing LMJ-SSP for metastatic status</strong></h4><p>A particularly novel aspect of Wood’s project is the use of LMJ-SSP to investigate differences associated with metastatic status.</p><p>She had previously worked on distinguishing healthy and cancerous tissues, where the biological differences are relatively large. Comparing two states within the same cancer type is more demanding because the molecular changes may be much subtler.</p><p>The scientific motivation, however, is strong. Tumors discovered at later stages carry greater concern for metastatic progression, making it important to understand the molecular changes associated with increasingly aggressive behavior.</p><p>For Wood, this made the methodological risk worthwhile: the analytical challenge directly addresses an area where better prognostic information could ultimately have clinical value.</p><h4><strong>A long-term goal: rapid MS-based prognostic testing</strong></h4><p>The long-term vision extends beyond cell-line research.</p><p>Wood is interested in whether rapid, minimally invasive mass spectrometry could eventually contribute to clinical decision-making.</p><p>One possibility would be identifying a diagnostic biomarker in a <strong>liquid biopsy</strong>, potentially using blood or urine. A urine-based marker capable of contributing to earlier detection would be particularly attractive for kidney cancer.</p><p>For prognostic applications, the goal would be slightly different. Once a tumor is discovered, molecular information could help characterize its likely behavior and provide clinicians with additional evidence when deciding between immediate treatment, surgery, or other management strategies.</p><p>Wood emphasizes that such a tool would not replace standard clinical workflows. Instead, it would provide additional molecular information to complement existing diagnostic and pathological approaches.</p><h4><strong>Why interdisciplinary work matters</strong></h4><p>Throughout the interview, Wood repeatedly returns to the importance of collaboration.</p><p>Her research sits at the intersection of analytical chemistry, pathology, oncology, computation, and clinical medicine. No single researcher can remain equally current in all of these fields.</p><p>Working closely with pathologists, computer scientists, surgeons, medical students, and other specialists therefore provides both scientific expertise and a way to stay connected with developments outside one’s immediate discipline.</p><p>For Wood, the biological context has also changed how she thinks about analytical chemistry. Developing a new instrument or method is important, but understanding <strong>who may ultimately benefit from that analytical capability</strong> makes the research more meaningful.</p><p>That patient-centered perspective is now an integral part of how she approaches the project.</p><h4><strong>An intersection full of opportunities</strong></h4><p>Wood describes the interface between analytical chemistry and oncology as an especially exciting area for young researchers.</p><p>The work can range from troubleshooting instrumentation to interpreting pathology, developing statistical methods, and collaborating across disciplines. That diversity creates constant opportunities to learn and to apply analytical chemistry to clinically relevant questions.</p><p>Her project illustrates that modern mass spectrometry research is increasingly about more than generating spectra. It involves connecting instrumentation, spatial sampling, multivariate statistics, machine learning, biological interpretation, and clinical needs into a single analytical strategy.</p><p>And in kidney cancer, where reliable prognostic biomarkers remain difficult to establish, that integrated approach may be particularly valuable.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

AIMS vs. LC-MS for Cancer Diagnostics: ccRCC Metabolomics with Rachel Wood

Article

Product

Scientists

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Science and research

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Waters SunFire C8 HPLC Columns

Waters SunFire C8 columns are used for general purpose method development at low pH.

<p><strong>SunFire C8 columns are used for general purpose method development at low pH. The C8 ligand is preferred for some assays because it is less hydrophobic and therefore less retentive compared to SunFire C18.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: SunFire C8 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C8</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L7</li><li><strong>Carbon Load:</strong> 12 %</li></ul><h4><strong>The Waters SunFire C8 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters SunFire C8 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: SunFire C8 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>SunFire Columns</strong></h3><h4><strong>Superior mass loading at low pH</strong></h4><p><strong>High efficiency at a low pH</strong> can be challenging. Waters SunFire HPLC Columns, available in <strong>analytical</strong> and <strong>preparative dimensions</strong>, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.</p><p><strong>SunFire Analytical Columns</strong> are available in <strong>2.5 µm</strong> to <strong>10 µm </strong>particle sizes across two reversed-phase column chemistries, <strong>C18</strong> and <strong>C8</strong>, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in <strong>5</strong> and <strong>10 µm particle sizes</strong> for <strong>preparative normal-phase purification</strong>.</p><h4><strong>Overview</strong></h4><ul><li><strong>Exceptional loading capacity </strong>for both analytical and preparative columns</li><li><strong>Excellent low pH stability </strong>for longer lifetimes</li><li><strong>Superior peak shapes </strong>for faster and easier integration</li><li><strong>Low mass spectral (MS) bleed</strong>, for enhanced analyte MS signal</li><li><strong>Seamless scalability from analytical</strong> to optimum bed density (OBD) <strong>preparative columns</strong></li><li><strong>Recommended Use:</strong> For increased mass loading with excellent column stability.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sun_Fire_Columns_3f48bd770c/Waters-SunFire-Columns.jpg" alt="Waters: SunFire Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sun_Fire_Columns_3f48bd770c/thumbnail_Waters-SunFire-Columns.jpg 242w," sizes="100vw" width="900" height="580"></p>

Waters ACQUITY APC Columns

Waters ACQUITY Advanced Polymer Chromatography (APC) Columns with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods.

<h4><strong>ACQUITY APC AQ BEH Column</strong></h4><p>ACQUITY APC AQ columns are designed to provide highly efficient size-based separations for water soluble polymer characterization. The sorbent uses patented Ethylene Bridge Hybrid (BEH) technology to provide a mechanical strong packed bed that resists shinking and swelling for easy mobile phase solvent switching. The expected molecular weight range for this column is approximatley 1, 000 - 30, 000 g/mole.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Separation Mode:</strong> SEC/GPC</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min:</strong> 1 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Medium</li><li><strong>Molecular Weight Range Min:</strong> 1000</li><li><strong>Molecular Weight Range Max:</strong> 30000</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Particle Size:</strong> 1,7 µm &nbsp;/ 2.5 µm&nbsp;</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>System:</strong> APC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>Technique: </strong>SEC</li></ul><h4><strong>ACQUITY APC XT BEH Column</strong></h4><p>ACQUITY APC XT columns are optimized to provide highly efficient size-based separations for organic solvent soluble polymer characterization. The sorbent uses patented Ethylene Bridge Hybrid (BEH) technology to provide a mechanical strong packed bed that resists shinking and swelling for easy mobile phase solvent switching.The expected molecular weight range for this column is approximatley 1,000 - 30,000 g/mole.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Separation Mode:</strong> SEC/GPC</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min:</strong> 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Molecular Weight Range Min:</strong> 1000</li><li><strong>Molecular Weight Range Max:</strong> 30000</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Particle Size:</strong> 1,7 µm &nbsp;/ 2.5 µm&nbsp;</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>System:</strong> APC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>Technique: </strong>GPC</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_AQ_BEH_Column_0c0d837b73.jpeg" alt="Waters: ACQUITY APC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_AQ_BEH_Column_0c0d837b73.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY APC Columns</strong></h3><h4><strong>Achieve superior speed and resolution for polymer characterization</strong></h4><p>Achieving superior performance and reliability with high resolution polymer characterization can be a challenge. <strong>ACQUITY Advanced Polymer Chromatography (APC) Columns</strong> are designed for polymer scientists like you, with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods. With these columns, you can quantify and characterize polymer samples with confidence and accuracy while maximizing productivity.</p><p>The <strong>Ethylene Bridged Hybrid (BEH) particles</strong> used in ACQUITY APC Columns enable rapid solvent switching and the ability to use multiple conditions for the same bank of columns. Conventional polymeric stationary phases are dedicated to a single set of analytical conditions and solvent because the chromatographic bed is vulnerable to shrinking and swelling. By eliminating this undesirable behavior, ACQUITY APC Columns allow for maximum robustness and method development flexibility.</p><h4><strong>Overview</strong></h4><ul><li>Eliminate the shrinking and swelling that often occurs with polymeric stationary phases upon solvent switching with a stable chromatographic column bed&nbsp;</li><li>Achieve unmatched separation speed, resolution, and chromatographic efficiency from optimized pore volumes and particle size</li><li>Meet workflow requirements with 1.7 and 2.5 μm columns for organic and aqueous size-based separation</li><li>Achieve industry-leading column-to-column reproducibility with exceptionally reliable columns from a tightly controlled column manufacturing process</li><li><strong>Recommended Use: </strong>For rapid and accurate chromatographic characterization of synthetic polymer and macromolecular species.&nbsp;</li></ul><h4><strong>ACQUITY APC Column Featured Technologies</strong></h4><h5><strong>BEH Technology</strong></h5><ul><li>Rugged hybrid organic/inorganic base particle</li><li>Improved peak shape for bases- low silanol activity</li><li>Wider operating pH range for method development flexibility</li><li>Mechanical strength for maximum column efficiency at UHPLC operating pressures</li></ul><h4><strong>ACQUITY APC Column Characteristics</strong></h4><h5><strong>ACQUITY APC XT Columns</strong></h5><ul><li>Designed to provide high efficiency sized-based separations for organic soluble polymer characterization</li><li>Uses BEH particle technology</li><li>Stable column bed that resists shrinking and swelling for easy mobile-phase solvent switching</li><li>Features an extended temperature range up to 90 °C</li><li>Designed for organic solvent-based separations</li></ul><h5><strong>ACQUITY APC AQ Columns</strong></h5><ul><li>Designed to provide high efficiency sized-based separations for water soluble polymer characterization</li><li>Uses BEH particle technology</li><li>Robust separations up to 45 °C</li><li>Designed for aqueous-soluble polymer separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_Columns_2268ceb5f5.jpeg" alt="Waters: ACQUITY APC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_Columns_2268ceb5f5.jpeg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Unprecedented capacity for rapid solvent switching</strong></h4><p>Use multiple conditions for the same column bank with rapid solvent switching using the rigid hybrid particles in ACQUITY APC Chromatography Columns. Conventional polymeric phases are often dedicated to single analysis conditions because the chromatographic bed is vulnerable to shrinking and swelling that can be potentially harmful to the column. By eliminating this constraint, ACQUITY APC Chromatography Columns provide more freedom for method development and deliver advanced polymer characterization for a diverse range of samples.</p><h4><strong>Reduce calibration time by a factor of 3-5x and increase resolution</strong></h4><p>With long conventional Gel Permeation Chromatography (GPC) run times of up to one hour or more, analyses of standards and samples can be quite time-consuming. In addition to long GPC run times, the large extra-column volume of conventional GPC systems can result in peak bandspreading, reducing the resolution and thus accuracy of the calibration points. Combined with the lower dispersion ACQUITY APC System, small particle ACQUITY APC Columns deliver less bandspreading and narrower, sharper peaks compared to the conventional GPC system. Additionally, robust sub-3-µm APC Column Technology supports higher flow rate and backpressures, thereby improving resolution.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_A_comparison_of_run_times_and_resolution_for_polystyrene_standards_Mp_100_K_10_K_and_1_K_on_a_conventional_GPC_system_and_the_ACQUITY_APC_System_37cfb15ed5.jpeg" alt="Waters: A comparison of run times and resolution for polystyrene standards (Mp: 100K, 10K, and 1K) on a conventional GPC system and the ACQUITY APC System. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_A_comparison_of_run_times_and_resolution_for_polystyrene_standards_Mp_100_K_10_K_and_1_K_on_a_conventional_GPC_system_and_the_ACQUITY_APC_System_37cfb15ed5.jpeg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Ensure reproducible column performance</strong></h4><p>Reproducible column performance is one of the most critical factors for achieving long-term method reliability during polymer characterization. Each step of our column manufacturing process is monitored and tightly controlled to maintain unmatched product reproducibility. Every test, from raw material characterization to final batch analysis, is designed to ensure that ACQUITY APC Columns perform the same from column-to-column, batch-to-batch, and year-to-year.</p>

Waters Sample & Collection Plate

Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots.

<div><div><h4><strong>Broad selection of high-quality sample and collection plates</strong></h4></div></div><div><div><p><strong>Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots. Waters plates are made from polypropylene (PP) for chemical resistance, and specialty 96-well plates with glass inserts are available for samples that are not suitable for polypropylene containers.</strong></p><h3><strong>Overview</strong></h3><div><div><div><div><div><div><ul><li>96- and 384-well collection plates and sample plates are SBS/ANSI compliant for robotic compatible systems&nbsp; &nbsp; &nbsp; &nbsp;</li><li>&nbsp;Constructed of polypropylene (PP) for chemical resistance</li><li>&nbsp;Glass inserts available for samples requiring glass surfaces</li><li>&nbsp;Heat seals and cap mats for plates and glass inserts</li></ul></div></div><div><div><h4><strong>Recommended Use</strong></h4><p>For general high-throughput LC and LC-MS work and for integration in (semi)-automated sample handling workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/img_Waters_Sample_and_Collection_Plate_709693fdab.jpg" alt="imgWaters Sample &amp; Collection Plate." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_img_Waters_Sample_and_Collection_Plate_709693fdab.jpg 245w," sizes="100vw" width="1024" height="493"></p><h3><strong>Cap Mats</strong></h3><h4><strong>Silicone/PTFE &amp; Polypropylene Cap Mats</strong></h4><ul><li>Non-slit storage mats for long-term sample storage</li><li>Pre-slit silicone/PTFE and polypropylene cap mats for use in autosamplers for proper venting and sample draw accuracy with excellent resealing characteristics</li><li>Silicone/PTFE &amp; Polypropylene Cap Mats</li></ul><h3><strong>Heat Seals</strong></h3><h4><strong>Clear Polyester Heat Seals</strong></h4><ul><li>Can be used with most solvents &amp; buffers over a -80 °C to 80 °C temperature range</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Aluminum Foil Heat Seals</strong></h4><ul><li>Made from a polyester/aluminum laminate</li><li>Ideal choice for long term storage to reduce evaporative loss</li><li>Aluminum layer reduces gas permeability of the seal</li><li>Can be used at temperatures from -200 °C to 90 °C</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Adhesive Seals</strong></h4><ul><li>A polyolefin film with synthetic rubber adhesive</li><li>Ideal for use with protein and peptide samples and buffers</li><li>Resistant to small concentrations (0% to 30%) of polar organic solvents</li><li>Can be used at temperatures from -80 °C to 80 °C</li><li>Requires a heat sealer to apply</li></ul></div></div></div></div></div></div></div></div>

Waters ACQUITY UPLC C18 Columns

ACQUITY UPLC columns offer unmatched speed, resolution, and peak shape for a wide range of analytes. With advanced particle technologies and broad pH stability, they support efficient, reproducible separations in both routine and demanding applications.

<h5><strong>ACQUITY UPLC BEH C18 Columns</strong></h5><ul><li>Universal C18 column</li><li>Suitable for a diverse range of analytes</li><li>High efficiency</li><li>Industry-leading mobile phase pH 1-12 and temperature compatibility</li></ul><h5><strong>ACQUITY UPLC CSH C18 Columns</strong></h5><ul><li>Universal column choice for UV and MS applications</li><li>Suitable for a broad range of compounds</li><li>Alternate selectivity to BEH C18</li><li>Exceptional peak shape and increased load capacity</li><li>Suited for basic compounds at low pH</li></ul><h5><strong>ACQUITY UPLC HSS T3 Columns</strong></h5><ul><li>Ideal for the enhanced retention of polar and non-polar compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Mechanically stable at elevated pressures</li></ul><h5><strong>ACQUITY UPLC HSS C18 Columns</strong></h5><ul><li>General purpose silica-based C18 chemistry</li><li>Suitable for a broad range of compounds</li><li>Provides exceptional peak shapes and low pH stability</li><li>Delivers increased retention compared to hybrid organic/inorganic C18 columns</li></ul><h5><strong>ACQUITY UPLC HSS C18 SB Columns</strong></h5><ul><li>Low coverage silica-based C18 chemistry</li><li>Provides alternate selectivity for compounds impacted by silanophilic interactions</li><li>Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes</li><li>Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)</li></ul><h4><strong>ACQUITY UPLC Application-Specific Column Characteristics</strong></h4><h5><strong>ACQUITY UPLC Peptide BEH C18 Columns</strong></h5><ul><li>Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH</li><li>Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns</li><li>Two pore sizes (130 Å and 300 Å) for a wide range of peptides</li></ul><h5><strong>ACQUITY UPLC Peptide CSH C18 Columns</strong></h5><ul><li>Outstanding peak capacities with formic acid for MS-based applications</li><li>Excellent performance with TFA for optical based applications</li><li>Less dependence on TFA or FA to achieve optimal peak capacity</li><li>Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities</li><li>At low pH with the positive charge on CSH, you get less secondary interactions with the particle</li></ul><h5><strong>ACQUITY UPLC Peptide HSS T3 Columns</strong></h5><ul><li>Separations that require silica-based selectivity</li><li>Separations that necessitate increased peptide retention</li><li>Ideally suited for small, hydrophilic peptides<br>&nbsp;</li></ul><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC BEH C8 Column

The Waters ACQUITY BEH C8 sorbent is less retentive compared to the ACQUITY BEH C18 sorbent. 

<p><strong>The ACQUITY BEH C8</strong> sorbent is <strong>less retentive compared to the ACQUITY BEH C18 </strong>sorbent. A method developer may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The <strong>trifunctionally bonded BEH particles</strong> offer the widest usable <strong>pH range (1-12)</strong>, <strong>superior low pH stability</strong>, and <strong>ultra-low column bleed</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C8</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Maximum Pressure: </strong>18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>130 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L7</li><li><strong>Carbon Load: </strong>13 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC BEH C8 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC Protein BEH C4 Column

The Waters ACQUITY UPLC Protein BEH C4 column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. 

<p><strong>The ACQUITY UPLC Protein BEH C4 </strong>column is QC tested and optimized to <strong>separate proteins</strong> based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the <strong>C4 ligand is less retentive </strong>and will <strong>minimize protein carryover,</strong> <strong>increase protein recovery</strong>, and <strong>improve peak capacity</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C4</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max: </strong>10 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L26</li><li><strong>Carbon Load: </strong>8 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: Kolona ACQUITY UPLC BEH C8" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC HSS CN Column

Waters ACQUITY UPLC HSS CN chemistry is an ultra-performance general purpose propyl cyano bonded phase that provides superior peak shape for bases, increased retention, and extremely long column lifetimes at low pH.

<p><strong>ACQUITY UPLC HSS CN</strong> chemistry is an ultra-performance general purpose <strong>propyl cyano bonded phase</strong> that provides <strong>superior peak shape</strong> <strong>for bases</strong>, <strong>increased retention</strong>, and <strong>extremely long column lifetimes at low pH</strong>. ACQUITY HSS CN columns are fully scalable and transferable to HPLC separations.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> Cyno (CN)</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>High</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> HSS</li><li><strong>USP Classification:</strong> L10</li><li><strong>Carbon Load: </strong>5 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC HSS CN Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC BEH Phenyl Column

The Waters ACQUITY BEH Phenyl columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds. 

<p><strong>ACQUITY BEH Phenyl </strong>columns provide <strong>complementary selectivity to C18</strong> stationary phases, <strong>especially for polyaromatic compounds</strong>. This sorbent, along with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1828"><strong>XBridge BEH Phenyl</strong></a>, provide you with the most chemiscally stable phenyl sorbent on the market and provides you with ultra-low column bleed, long column lifetimes and excellent peak shape.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> Phenyl</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>130 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L11</li><li><strong>Carbon Load: </strong>15 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC BEH Phenyl Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters CORTECS Shield RP18 HPLC Columns

Waters CORTECS Shield RP18 columns provides complimentary selectivity to C8 and C18 stationary phases. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds.

<p><strong>CORTECS Shield RP18</strong> columns provides <strong>complimentary selectivity to C8 </strong>and <strong>C18</strong> stationary phases. The <strong>embedded carbamate group</strong> in the bonded phase ligand provides <strong>alternate selectivity, especially for phenolic compounds</strong> compared to straight chain alkyl columns. The solid core particle morphology offers high efficiency and lower backpressures when compared to fully porouse particles of similar particle size.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 6.4 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS Shield RP18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters CORTECS C18+ HPLC Columns

Waters CORTECS C18+ columns are general purpose, charged surface, high efficiency C18 columns based on a solid-core particle.

<p><strong>CORTECS C18+</strong> columns are general purpose, charged surface, high efficiency C18 columns based on a solid-core particle that is designed to deliver excellent peak shape for basic analytes at low pH.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode:</strong> Reversed phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min</strong>: 2 pH</li><li><strong>pH Range Max:</strong> 8 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>90 Å</li><li><strong>System: </strong>HPLC</li><li><strong>Particle Technology:</strong> Solid core</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon load:</strong> 5.7 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CORTECS_C8_Column_68f0f00415.jpg" alt="Waters: CORTECS C18+ Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CORTECS_C8_Column_68f0f00415.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>CORTECS Columns</strong></h3><h4><strong>Solid-core columns that live up to their potential.</strong></h4><p>Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of <strong>CORTECS solid-core columns</strong>, which provide the flexibility and power you need to rapidly separate a wide array of compounds.</p><p><strong>CORTECS Columns</strong> are available in <strong>reversed-phase and HILIC options</strong> across <strong>seven column chemistries</strong> with <strong>different particle characteristics</strong> and innovative charged surface modifications for 20% faster separations and 25% more resolution at low operating back pressures.</p><h4><strong>Overview</strong></h4><ul><li>Maximize efficiency with <strong>high-performance columns</strong> created using state-of-the-art column packing techniques, bonding and end-capping processes.</li><li>Achieve <strong>greater throughput and speed</strong> than fully porous particle columns.</li><li>Get <strong>simple and seamless transfer</strong> with consistent results across different particle sizes and column configurations.</li><li><strong>Flexibly develop methods for challenging separations</strong> faster with reversed-phase and HILIC options across seven column chemistries.</li><li><strong>Prolong column lifetimes</strong> with optional VanGuard Cartridges and pre-columns specifically designed using CORTECS stationary phases.</li><li><strong>Recommended Use:</strong> To maximize resolution and speed for all LC separations.</li></ul><h4><strong>CORTECS Column Characteristics</strong></h4><h5><strong>CORTECS C18+ Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>A positively charged surface delivers excellent peak shape for basic compounds at low pH.</li></ul><h5><strong>CORTECS C18 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Balanced retention of acids, bases, and neutrals at low and mid-range pH.</li></ul><h5><strong>CORTECS C8 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Similar selectivity but shorter retentivity when compared to C18 columns.</li></ul><h5><strong>CORTECS T3 Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>Reversed-phase column</li><li>Enables to use of 100% aqueous mobile phase and increased retention of polar compounds.</li></ul><h5><strong>CORTECS Phenyl Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for polyaromatic compounds.</li></ul><h5><strong>CORTECS Shield RP18 Columns</strong></h5><ul><li>High efficiency</li><li>Method development column</li><li>Provides alternate selectivity, particularly for phenolic compounds.</li></ul><h5><strong>CORTECS HILIC Columns</strong></h5><ul><li>General purpose</li><li>High efficiency</li><li>HILIC Column</li><li>Offers orthogonal selectivity when compared to reversed-phase columns.</li></ul><h4><strong>Reliable columns. Consistent performance.</strong></h4><p>Get consistent batch-to-batch performance year after year with reliable CORTECS Columns.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg" alt="Waters: Consistent performance" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Get_consistent_batch_to_batch_reproducibility_with_long_column_lifetimes_4600807215.jpg 227w," sizes="100vw" width="1024" height="704"></p><h4><strong>Method transfer just got easier.</strong></h4><p>Get the versatility you need for seamless method transfer, with consistent results and fully scalable particle sizes for HPLC, UHPLC, and UPLC platforms.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg" alt="Waters: Modernize and speed up your existing methods." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Modernize_and_speed_up_your_existing_methods_2dd7e61390.jpg 191w," sizes="100vw" width="1024" height="837"></p><h4><strong>Increased resolution and increased throughput.</strong></h4><p>Maximize column efficiency and throughput with increased resolution for the toughest samples on any LC system.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Maximize_Column_Efficiency_69800e15cb.jpg" alt="Waters: Maximize Column Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Maximize_Column_Efficiency_69800e15cb.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Products focused on Consumables - page 14

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