<p><span><strong>Commercial and public spectral libraries provide broad coverage for LC-MS/MS identification, but they cannot contain every compound that matters to a specific laboratory. Proprietary products, metabolites, intermediates, impurities, degradation products, biomarkers, and internally characterized compounds may be highly relevant even if they are absent from standard reference collections. NIST26 allows laboratories to build their own MS/MS user libraries and integrate them directly into routine search workflows, creating a focused resource tailored to their analytical needs.</strong></span></p><h4><span><strong>Why Create a User MS/MS Library?</strong></span></h4><p><span>User libraries are usually much smaller than commercial or public spectral databases, but their value is not determined by size alone.</span></p><p><span>A laboratory-specific library may contain exactly the compounds most important for a particular application. These can include:</span></p><ul><li><span>proprietary products,</span></li><li><span>known metabolites,</span></li><li><span>synthesis intermediates,</span></li><li><span>impurities,</span></li><li><span>degradation products,</span></li><li><span>biomarkers,</span></li><li><span>compounds associated with specific industrial or environmental samples.</span></li></ul><p><span>Such libraries can therefore provide highly relevant identifications in pharmaceutical development, industrial R&amp;D, medical laboratories, environmental analysis, and other specialized fields. A relatively small collection of carefully characterized compounds may be more useful for a particular laboratory than a much larger general-purpose database.&nbsp;</span></p><h4><span><strong>What Information Can Be Stored with Each Spectrum?</strong></span></h4><p><span>Creating a useful library entry involves more than importing an MS/MS spectrum.</span></p><p><span>A complete record can combine several types of information, including the experimental spectrum, chemical structure, instrumental parameters, ionization information, sample history, and additional user-defined metadata.</span></p><p><span>Spectra can be transferred from vendor software into NIST Library Search, or they can be sent from the Chromatogram tab directly to the Library Search environment in NIST26. Chemical structures can be imported through the Windows clipboard from a molecular drawing program, while standard experimental parameters and sample-history information can be prepared separately and added to the entry.&nbsp;</span></p><p><span>The ability to keep analytical metadata together with the spectrum is particularly important for internally generated libraries because it preserves the context in which the reference spectrum was acquired.</span></p><h4><span><strong>Using Tags to Organize LC-MS/MS Metadata</strong></span></h4><p><span>NIST26 supports a range of structured tags for LC-MS/MS spectra.</span></p><p><span>These can describe parameters such as:</span></p><ul><li><span>spectrum type,</span></li><li><span>compound type,</span></li><li><span>ion name,</span></li><li><span>precursor type,</span></li><li><span>precursor </span><i><span>m/z</span></i><span>,</span></li><li><span>collision energy,</span></li><li><span>instrument type,</span></li><li><span>sample identifier,</span></li><li><span>ionization mode,</span></li><li><span>ion polarity,</span></li><li><span>collision gas,</span></li><li><span>pressure,</span></li><li><span>mass range,</span></li><li><span>maximum intensity,</span></li><li><span>cone voltage,</span></li><li><span>charge,</span></li><li><span>molecular weight,</span></li><li><span>known impurities,</span></li><li><span>related CAS numbers,</span></li><li><span>peptide sequence and peptide modifications.</span></li></ul><p><span>This provides a convenient way to attach searchable and standardized experimental information to spectra rather than leaving all metadata in an unstructured comment field.&nbsp;</span></p><p><span>Tags can be entered individually through the <strong>Additional Info</strong> section in the Librarian interface, but when many spectra are being added, a grouped approach can be considerably faster.&nbsp;</span></p><h4><span><strong>Adding Groups of Tags Efficiently</strong></span></h4><p><span>For repetitive workflows, metadata can be prepared in advance and pasted into NIST26 as a group.</span></p><p><span>The presentation recommends maintaining frequently used tag sets in a simple text document, then copying them into the synonym field and using <strong>Add to List</strong> to convert them into structured information.</span></p><p><span>This is especially useful when the vendor software already transfers some parameters automatically. Users can then add only the missing information, such as ion adduct or polarity, rather than duplicating metadata already imported with the spectrum.&nbsp;</span></p><p><span>Care is required because entering information that is already present may create duplicate metadata.</span></p><p><span>This workflow can substantially accelerate library creation when many spectra share the same instrument configuration or acquisition parameters.</span></p><h4><span><strong>Preserving Sample History</strong></span></h4><p><span>A spectral match is more useful when the origin of the reference spectrum is known.</span></p><p><span>NIST26 therefore allows detailed sample information to be stored in the comment field. For batches of related samples, the text can be prepared in Notepad or another editor and pasted into multiple records.</span></p><p><span>Examples of useful information include sampling location, sampling date and time, analyst name, organization, internal sample identifier, project information, or other relevant context.&nbsp;</span></p><p><span>For environmental, clinical, pharmaceutical, and industrial laboratories, this information can become an important part of data traceability.</span></p><h4><span><strong>Adding Molecular Structures</strong></span></h4><p><span>A user library becomes considerably more useful when spectra are linked to molecular structures.</span></p><p><span>Structures can be created from scratch in a chemical drawing program. Alternatively, a similar structure can be located in NIST Search and sent to the default structure editor, where it can be modified as needed.</span></p><p><span>Once completed, the structure can be copied to the Windows clipboard and inserted into the NIST Librarian spectrum-information window. NIST26 can then calculate the molecular formula and molecular weight from the imported structure.&nbsp;</span></p><p><span>This linkage between spectrum and structure supports more informative library displays and structure-based searching.</span></p><h4><span><strong>Creating Custom User-Defined Tags</strong></span></h4><p><span>In addition to the standard LC-MS/MS tags, laboratories can define their own metadata fields.</span></p><p><span>Examples shown include identifiers such as:</span></p><ul><li><span>chemist,</span></li><li><span>laboratory notebook number,</span></li><li><span>analyst.</span></li></ul><p><span>The information can be prepared in a text editor and added using the appropriate tag syntax. Once configured in the <strong>Comment Field Display</strong> settings, these values are displayed as separate structured lines rather than remaining embedded in the general comments.&nbsp;</span></p><p><span>This flexibility makes the library more suitable for laboratories with established internal documentation systems or quality-management requirements.</span></p><p><span>NIST can also automatically add information such as the InChIKey and an estimated retention index when appropriate structural information is available.&nbsp;</span></p><h4><span><strong>High-Resolution and Low-Resolution Libraries Should Be Kept Separate</strong></span></h4><p><span>User libraries do not have to contain only high-resolution spectra.</span></p><p><span>Low-resolution MS/MS spectra may also be valuable, including spectra generated by:</span></p><ul><li><span>in-source collision on single-quadrupole instruments,</span></li><li><span>triple-quadrupole MS/MS,</span></li><li><span>other nominal-mass workflows.</span></li></ul><p><span>The recommendation is to maintain <strong>separate high-resolution and low-resolution user libraries</strong>, because the appropriate search strategies and handling can differ.&nbsp;</span></p><p><span>Low-resolution spectra can still generate strong library-search results and may be particularly useful when laboratories want to preserve historical data or incorporate spectra from multiple instrument types.</span></p><h4><span><strong>Important Software Considerations When Importing Spectra</strong></span></h4><p><span>The presentation also highlights several practical software issues that users should be aware of.</span></p><p><span>One concerns the <strong>Intensity Threshold</strong> setting in the Spectrum Import Options window. When an intensity threshold is applied during import from vendor software, high-resolution spectral values may be converted to nominal mass rather than preserving the expected decimal precision.</span></p><p><span>For laboratories building high-resolution user libraries, this is an important detail because accurate-mass information is one of the main advantages of HRMS data.</span></p><p><span>Another issue affects some library-search modes after high-resolution spectra are added to a user library.</span></p><h4><span><strong>HiRes No Precursor and Hybrid Search Limitations</strong></span></h4><p><span>After adding high-resolution spectra, certain <strong>HiRes NoPrecursor</strong> and <strong>Similarity Hybrid MS/MS</strong> searches may not function correctly.</span></p><p><span>A workaround involves exporting the affected library using <strong>Lib2NIST</strong> into MSP ASCII files together with the associated molecular structure files, then importing the data back into NIST library format.&nbsp;</span></p><p><span>The presentation specifically warns against attempting the problematic MS/MS Hybrid Search under these conditions because it may cause the software to crash.</span></p><p><span>An EI Normal search can mimic some of the functionality of HiRes No Precursor and may work effectively when high- and low-resolution libraries are searched together. HiRes NoPrecursor can also operate with presearch disabled, although this can make searching considerably slower.&nbsp;</span></p><h4><span><strong>Display Issues in the Chromatogram Window</strong></span></h4><p><span>Another software behavior concerns how spectral values are displayed in the Chromatogram tab.</span></p><p><span>Values from Spectrum Import Options are reflected in the head-to-head spectral display. If settings are adjusted for low-resolution spectra, the displayed masses may appear truncated.</span></p><p><span>Importantly, this visual behavior does <strong>not</strong> affect the results in the accurate-mass deconvolution and library-searching table; it affects only the spectral display at the top of the Chromatogram window.&nbsp;</span></p><p><span>Distinguishing display behavior from actual processing results is important to avoid misinterpreting valid search results as corrupted data.</span></p><h4><span><strong>Updating the Library After Adding New Entries</strong></span></h4><p><span>User-library maintenance does not end when a spectrum has been added.</span></p><p><span>Whenever a new entry containing a molecular structure is introduced, the <strong>structure index should be updated</strong> so that the new compound can be included properly in structure searching.</span></p><p><span>The InChIKey appears to update with the structure, but if duplicate records are not being handled correctly, the InChIKey index may also need to be rebuilt.&nbsp;</span></p><p><span>If a new library folder is added to the NIST26 MSSEARCH directory while the software is already running, users should also update the library list so that the newly added collection becomes available without restarting the entire environment.&nbsp;</span></p><h4><span><strong>Using Lib2NIST to Repair High-Resolution User Libraries</strong></span></h4><p><span>The <strong>Lib2NIST</strong> utility plays an important role in resolving some high-resolution library-search issues.</span></p><p><span>The workflow involves exporting the problematic high-resolution library to an MSP file together with its molecular structure files and then re-importing the data into NIST library format.</span></p><p><span>The corrected library can then be placed back into the NIST26 MSSEARCH directory. It is advisable to preserve the original library under a different name until the repaired version has been tested successfully.&nbsp;</span></p><p><span>The presentation also recommends closing NIST Search while performing Lib2NIST conversions.</span></p><h4><span><strong>Small Libraries Can Deliver High Analytical Value</strong></span></h4><p><span>The central message is straightforward: a user library does not need to contain hundreds of thousands of compounds to be useful.</span></p><p><span>A carefully curated collection of spectra from compounds that repeatedly appear in a laboratory's own samples can dramatically improve identification relevance. This is especially valuable for compounds that are proprietary, newly synthesized, application-specific, or otherwise poorly represented in public and commercial databases.</span></p><p><span>By combining MS/MS spectra with structures, acquisition metadata, sample history, tags, and internally defined information, NIST26 can become more than a search engine for commercial databases. It can serve as a laboratory-specific knowledge base built around compounds that genuinely matter to the user's work.</span></p><p><span>For specialized LC-MS/MS workflows, that may be one of the most valuable ways to extend the capabilities of spectral library searching.</span></p>

Creating MS/MS User Libraries in NIST26

<p><strong>Wiley announces the expansion of its spectral libraries across IR and Raman, adding empirical and computed reference data spanning forensics, pigments and dyes, mineralogy, materials, polymers, and pharmaceuticals. This release adds over a thousand new empirical reference spectra, plus more than 429,000 new SmartSpectra records, across dozens of updated and new databases. This brings the combined collections to over 1.4 million IR spectra and 230,000 Raman spectra.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Wiley_continues_to_expand_its_IR_Raman_and_Smart_Spectra_spectral_libraries_806b34cdc1.jpg" alt="Wiley: Wiley continues to expand its IR, Raman, and SmartSpectra spectral libraries" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Wiley_continues_to_expand_its_IR_Raman_and_Smart_Spectra_spectral_libraries_806b34cdc1.jpg 245w," sizes="100vw" width="1600" height="838"></p><p>“<i>Scientific discoveries and business decisions depend on trustworthy analytical results,</i>” said <strong>Graeme Whitley</strong>, senior director, data science solutions at Wiley. “<i>The value of a spectral library expansion is not simply that it adds more records. It gives analysts more relevant reference candidates when they encounter unfamiliar materials, whether they are verifying a polymer, investigating a pharmaceutical sample, examining a mineral, or screening a forensic specimen.</i>”</p><h4><strong>Highlights include:</strong></h4><ul><li><strong>Forensics:</strong> A new Raman forensics database adds reference spectra for colorant and dye identification on textiles, drawn from real fiber-evidence research, useful for investigators working on dye and textile analysis.</li><li><strong>Pigments and dyes:</strong> New Raman and IR data significantly broadens pigment and dye coverage, including historic artists' pigments, supporting art conservation and cultural-heritage authentication work.</li><li><strong>Minerals: </strong>New Raman and IR data extends coverage to minerals from different localities and Martian mineral analogs. This release includes new Raman additions from our ongoing collaboration with the American Museum of Natural History, now cross-referenced with DANA Classification Numbers and Mindat IDs, giving mineralogists and gemologists an easier way to identify entries against established references.</li><li><strong>Materials and semiconductors: </strong>New Raman and IR data covers crystal growth relevant to battery materials, rare-earth-doped magnetic crystals, and flexible and 2D semiconductor materials, useful for researchers in battery, magnetic materials, and electronics work.</li><li><strong>Polymers: </strong>New Raman and IR data spans 3D-printed, MOF-integrated microlattices, environmental plastic aging and recycled PET materials, and food packaging plastics. New Raman reference spectra also cover more than two dozen synthetic polymer types for identifying microplastics in environmental samples.</li><li><strong>Clinical and pharma:</strong> New Raman and IR data covers antibiotic classes, including beta-lactams, and the WHO's essential antibiotic list, plus bone mineralization studies in clinical models. A new IR dataset also adds spectra for common over-the-counter medications, including aspirin, ibuprofen, and paracetamol, helping quality control teams confirm formulations faster.</li><li><strong>SmartSpectra: </strong>Our SmartSpectra IR, Raman, and vapor-phase IR collections continue to grow, adding<strong> 243,000+ IR</strong>, <strong>67,000+ Raman</strong>, and <strong>119,000+ vapor-phase IR</strong> computed reference spectra to support broad, high-throughput identification.</li></ul><blockquote><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/spectral-databases/"><strong>Learn more about Wiley Spectral Databases</strong></a></p><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/how-to-update-knowitall-databases/"><strong>Existing subscribers can find guidance on updating their databases here</strong></a></p></blockquote><h4><strong>Wiley Spectral Databases</strong></h4><p>Wiley's spectral databases are consistently evolving to meet today's research demands, covering multiple spectral techniques across a wide range of applications including polymers, materials, environmental, forensics, toxicology, pharmaceutical, biotech, automotive, aerospace, food, cosmetics, and more. Built on rigorous quality protocols, Wiley delivers industry-leading spectral data and intelligent tools to accelerate analysis and deliver deeper insights — making it the authoritative, trusted source that analytical labs worldwide rely on.&nbsp;</p>

Wiley continues to expand its IR, Raman, and SmartSpectra spectral libraries

<p><strong>Per- and polyfluoroalkyl substances (PFAS)&nbsp;analysis is no longer&nbsp;defined by&nbsp;a fixed list of known compounds.</strong></p><p>As regulatory frameworks evolve and scientific understanding deepens, laboratories are being asked to go beyond routine testing. In addition to quantifying known PFAS, they must also investigate unknown, unexpected, and emerging compounds that fall outside predefined target lists.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_509ce49fc2.webp" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_509ce49fc2.webp 245w," sizes="100vw" width="1024" height="526"></p><p>This shift introduces a fundamental analytical challenge.</p><p>Targeted quantitation provides robust and validated concentration data for known PFAS. However, by design, it only answers questions about compounds already included in the method. In contrast, non-targeted analysis,&nbsp;using high-resolution accurate-mass&nbsp;(HRAM)&nbsp;data,&nbsp;enables scientists to explore the broader chemical landscape, revealing&nbsp;additional&nbsp;PFAS&nbsp;that were not included in a targeted quantitation method.</p><p>For PFAS, where thousands of structurally related compounds and transformation products may be present, neither approach&nbsp;alone provides a complete picture.</p><p>The&nbsp;real challenge&nbsp;lies in connecting these workflows. Discovery findings must be translated into actionable results that can be reviewed, followed up, and reported within a controlled analytical framework.</p><p><i>In a previous<strong>&nbsp;</strong></i><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7167"><i><strong>blog post</strong></i></a><i>, we introduced how </i><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759"><i><strong>Thermo Scientific Compound Discoverer software </strong></i></a><i>and </i><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/76"><i><strong>Thermo Scientific Chromeleon Chromatography Data System&nbsp;(CDS)</strong>&nbsp;</i></a><i>connect discovery and routine workflows.&nbsp;Here, we explore what that connection looks like in practice using PFAS analysis as an example.</i></p><h3><strong>Why PFAS&nbsp;requires&nbsp;more than one analytical question</strong></h3><p>PFAS&nbsp;represent a&nbsp;large&nbsp;and diverse class of compounds,&nbsp;with&nbsp;some&nbsp;estimates exceeding<strong>&nbsp;40,000 individual substances</strong>. Regulatory methods typically focus on a limited subset, yet environmental and biological samples often&nbsp;contain&nbsp;many more&nbsp;PFAS, including precursors and transformation products.</p><p><strong>This creates two&nbsp;essential and&nbsp;complementary analytical questions:</strong></p><p style="text-align:center;"><i>How much of these known PFAS are present?</i></p><p style="text-align:center;">and</p><p style="text-align:center;"><i>What other PFAS-related compounds might be present?</i></p><p>A complete workflow needs to support both&nbsp;questions.</p><h3><strong>Starting with targeted quantitation</strong></h3><p>For known PFAS, targeted quantitation&nbsp;remains&nbsp;the&nbsp;backbone of routine analysis.</p><p>Chromeleon CDS provides&nbsp;a&nbsp;controlled environment for&nbsp;data acquisition, processing, calibration, and reporting. In a PFAS workflow,&nbsp;this supports routine quantitation of predefined targets using laboratory-specific method and review criteria.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_2_7ff8eda8fe.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 1. Targeted quantitation of known PFAS in Chromeleon CDS.&nbsp;This example is&nbsp;from the analysis of PFAS using a&nbsp;Thermo Scientific&nbsp;Orbitrap Exploris&nbsp;240. Here&nbsp;we are showing&nbsp;the&nbsp;calibration curve (right) and extracted&nbsp;ion chromatogram&nbsp;(left)&nbsp;of 7:3 FTCA&nbsp;at 6 ng/L.&nbsp;The bottom&nbsp;half&nbsp;shows the processing method that&nbsp;contains&nbsp;many target PFAS and&nbsp;isotopically-labelled&nbsp;internal standards." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_2_7ff8eda8fe.jpg 208w," sizes="100vw" width="794" height="596"></p><p>This targeted workflow is essential, but it is only part of the story. A sample may contain PFAS-related compounds not included in the original method.</p><h3><strong>Looking beyond known PFAS</strong></h3><p>Once HRAM data have been acquired, the same dataset can be explored beyond the target list using Compound Discoverer software. This enables comprehensive analysis of complex datasets, including:</p><ul><li>Detection and alignment of unknown features</li><li>Statistical comparison across samples and blanks</li><li>Spectral library searching and database annotation</li><li>Evaluation of isotope patterns, fragmentation, and homologous series</li></ul><p>For PFAS, this is particularly valuable. The software can leverage characteristic properties such as negative mass defect, CF₂-based homologous series, and diagnostic fragment ions to prioritize PFAS-related candidates and assign confidence to putative identifications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_3_e523e1cdc9.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 2. Discovery of additional PFAS-related components in Compound Discoverer software. Here we show an example of using PFAS fragment libraries, one of many PFAS-specific resources available within Compound Discoverer software, to annotate fragments within acquired MS2 spectra of two unknown compounds and then identify them as PFAS that were outside the targeted quantitation method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_3_e523e1cdc9.jpg 233w," sizes="100vw" width="1520" height="1016"></p><p>The goal of discovery is not simply to generate more data. It is to reduce complexity and identify the most relevant candidates for further investigation with a defined level of confidence.</p><h3><strong>From discovery to follow-up</strong></h3><p>One of the most important questions in non-targeted analysis is what happens after candidate identification.</p><p>Discovery workflows can identify interesting candidates, but those candidates need to move into a practical follow-up process. Scientists may want to monitor them across additional samples, estimate relative or semi-quantitative abundance, trend their presence over time, or include them in a reviewable report.</p><p>This is where the connection between Compound Discoverer software and Chromeleon CDS becomes especially valuable.</p><p>After PFAS candidates have been prioritized in Compound Discoverer software, selected components can be transferred back into Chromeleon CDS and incorporated into the Chromeleon processing methods for follow-up analysis. This allows scientists to evaluate these compounds within the same controlled environment used for targeted quantitation.</p><p>For PFAS analysis, this may support semi-quantitative follow-up for compounds where authentic standards are not yet available. While fully quantitative results require appropriate standards and validated methods, semi-quantitative workflows can provide useful estimates or relative comparisons to help prioritize further investigation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_4_8ee8ebc2df.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 3. Prioritized PFAS candidates brought back into Chromeleon CDS for follow-up. Prioritized PFAS candidates from Compound Discoverer software can be brought back into Chromeleon CDS for follow-up review, semi-quantitative evaluation, and controlled reporting." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_4_8ee8ebc2df.jpg 245w," sizes="100vw" width="1512" height="751"></p><p>This is the key workflow advantage: discovery does not stop at identification. It can feed directly into a practical analytical process.</p><h3><strong>Why this matters</strong></h3><p>PFAS analysis increasingly requires laboratories to balance routine testing with exploratory investigation.</p><p>A connected workflow between Chromeleon CDS and Compound Discoverer software supports this by:</p><ul><li>Combining targeted quantitation and non-targeted discovery to characterize samples more completely</li><li>Reducing manual data transfer and workflow fragmentation</li><li>Enabling discovery results to be reviewed and reported within a controlled CDS environment</li><li>Supporting investigation of emerging PFAS investigations without immediate method redevelopment</li><li>Bridging research-driven insights with routine analytical workflows</li></ul><p>This reflects a broader shift in analytical science, where the value lies not only in generating data, but in connecting workflows and turning data into actionable information.</p><h3><strong>Moving PFAS analysis forward</strong></h3><p>PFAS analysis is evolving from a fixed target list toward a more comprehensive understanding of complex chemical mixtures.</p><p>Laboratories must quantify known compounds while remaining prepared to identify and evaluate what lies beyond them.</p><p>By connecting <strong>Compound Discoverer software </strong>with<strong> Chromeleon CDS</strong>, laboratories can move seamlessly from targeted quantitation to discovery and into structured follow-up. The result is a more complete view of PFAS contamination and a more efficient path from data to insight.</p><h3><strong>Learn more</strong></h3><p>Explore how <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759"><strong>Compound Discoverer software</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/76"><strong> Chromeleon CDS </strong></a>can help connect targeted quantitation and non-targeted discovery workflows for <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/environmental/environmental-contaminant-analysis/emerging-contaminants/pfas-testing.html"><strong>PFAS analysis</strong></a>.</p><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a><strong>&nbsp;</strong>#PFAS #Chromeleon #CompoundDiscoverer #Environmental</p></blockquote>

PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow

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Phenomenex Zebron ZB-35HT Inferno GC Columns

Zebron Inferno column ZB-35HT is the first non-metal, 35 % phenyl column stable to 400 ˚C.

<h4><strong>High Temperature Stability for Mid-Polarity</strong></h4><ul><li>First non-metal, 35 % phenyl columns stable to 400 ˚C</li><li>Longer lifetime with rugged high temperature, polyimide coated, fused silica tubing</li><li>Robust column for high temperature analysis</li><li>Great for high molecular weight compounds</li><li>Eliminate carry-over with high temperature bake outs</li><li>Low activity, provides good peak shape for acidic and basic samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_35_HT_Inferno_GC_Column_773b8b1796.webp" alt="Phenomenex: Zebron ZB-35HT Inferno GC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_35_HT_Inferno_GC_Column_773b8b1796.webp 245w," sizes="100vw" width="500" height="150"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_35_HT_Inferno_79e3acc7c9.jpg" alt="Phenomenex: Zebron™ ZB-35HT Inferno™" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_35_HT_Inferno_79e3acc7c9.jpg 167w," sizes="100vw" width="603" height="564"></p><h4><strong>Recommended Use</strong></h4><p><strong>Intermediate polarity with high temperature stability (up to 400 °C) for:</strong></p><ul><li>high boilers</li><li>contaminants</li><li>carry-overs</li><li>high-temp bakeouts</li></ul><h4><strong>Recommended Applications</strong></h4><ul><li>Amines</li><li>Aroclors</li><li>Chemicals</li><li>Drugs of Abuse</li><li>EPA Methods 508, 608, 8081, 8141, 8151</li><li>Pesticides</li><li>Pharmaceuticals</li><li>Semi-volatiles</li><li>Steroids</li></ul><h5><strong>Alternative to:</strong> Any 35 % Phenyl 65 % Dimethylpolysiloxane Phase:</h5><ul><li>DB®-35ms</li><li>SPB-680</li><li>MDN-35</li><li>DC-35</li><li>Rtx-35&nbsp;</li><li>SPB®-35</li><li>Rtx®-35MS</li><li>007-11</li><li>EC-35</li><li>BPX35</li><li>AT-35</li><li>HP-35ms</li><li>Sup-Herb</li><li>OV-11</li><li>HP-35</li><li>BPX608</li></ul><h3><strong>Phenomenex Zebron Inferno GC Columns</strong></h3><h4><strong>Overcome Traditional Obstacles</strong></h4><h5><strong>Challenge # 1: Short Fused Silica Column Lifetimes</strong></h5><p>Unlike Zebron™ Inferno™ GC columns, traditional fused silica columns are not equipped to handle prolonged exposure to<br>temperatures above 360 °C. At high temperatures, fused silica GC columns:</p><ul><li>Become inflexible and brittle</li><li>Break easily and cause costly replacement</li><li>Require more system downtime for maintenance</li><li>Can’t separate high molecular weight compounds</li></ul><h5><strong>Challenge # 2: Cumbersome Metal Column Tubing</strong></h5><p>Though metal columns have higher temperature limits than capillary columns, they are more difficult to use.</p><p><strong>Most metal columns:</strong></p><ul><li>Are inflexible and hard to use</li><li>Require special tubing cutters for installation</li><li>Are prone to leaks from expansion and contraction</li><li>Have active surfaces, giving poor peak shapes for acids and bases</li><li>Are incompatible with MS detectors</li></ul><h3><strong>How it works</strong></h3><p>Zebron™ Inferno™ columns offer enhanced performance where other columns fail. The two critical factors that allow for this are:</p><ul><li><h5><strong>Advanced ESC™ Bonding Technology</strong></h5></li></ul><p>At high temperature ranges, the stability of standard GC columns deteriorates, resulting in increased bleed. Zebron’s Engineered Self Cross-linking™ (ESC) bonding technology reinforces the stationary phase for enhanced column durability and extremely low bleed levels at high temperatures. <strong>The result:</strong> Inferno columns with the flexibility to perform for high temp oven ramps and bakeouts.</p><ul><li><strong>High Temperature Polyimide Coating</strong></li></ul><p>Standard polyimide resin pyrolyzes at temperatures above 360 °C, making the tubing unstable. Zebron Inferno columns use a temperature resistant polyimide resin that shows minimal thermal degradation, even at temperatures up to 430 °C*. This means longer column lifetime at elevated temperatures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg" alt="Phenomenex: Zebron Inferno GC Columns - How it works" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg 245w," sizes="100vw" width="978" height="564"></p><h3><strong>Features of Zebron Inferno GC Columns</strong></h3><h4><strong>Extremely Low Bleed for Rugged Performance</strong></h4><ul><li>Longer column lifetime</li><li>Better reproducibility</li><li>Maximum sensitivity</li><li>More accurate quantitation</li></ul><h4><strong>Increased Column Lifetime with Enhanced Durability</strong></h4><ul><li>Less instrument downtime</li><li>More productivity</li><li>Lower laboratory costs</li></ul><h5><strong>Standard GC Columns Cannot Withstand High Temperature</strong></h5><p>After prolonged exposure to temperatures above 360 °C, most traditional GC columns become brittle and inflexible, often spontaneously breaking in the middle of a run. Work is stopped in order to change out the column and perform system maintenance. Unlike these fragile columns, ZebronTM InfernoTM columns have a thermally resistant polyimide coating for longer column lifetime at temperatures up to 430 °C. As a result, you get less instrument downtime, and higher productivity.</p><h4><strong>Greater Performance for High Boiling Compounds</strong></h4><ul><li>Better peak shape</li><li>Improved separation</li><li>More analytes detected</li></ul><p>Zebron™ Inferno™ columns have the inertness and temperature stability needed to separate high molecular weight compounds. These high boilers require a thermally rugged column with low bleed levels. Inferno columns provide optimal separation of these analytes.</p>

Phenomenex Zebron ZB-XLB-HT Inferno GC Columns

Zebron ZB-XLB-HT Inferno is a rugged, non-metal si-arylene GC column stable to 400 ˚C

<h4><strong>High Temp Stability, Low Bleed</strong></h4><ul><li>Rugged, non-metal si-arylene GC column stable to 400 ˚C</li><li>Robust column for high temperature bake outs and analysis, such as high molecular weight compounds</li><li>Provides unique selectivity for conformational analyses</li><li>Longer lifetime with high temperature, polyimide coated, fused silica tubing</li><li>Low activity, provides good peak shape for acidic and basic samples</li><li>Good tool for general screening to identify unknown samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_XLB_HT_Inferno_9541a754ac.jpg" alt="Phenomenex: Zebron ZB-XLB-HT Inferno" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_XLB_HT_Inferno_9541a754ac.jpg 165w," sizes="100vw" width="599" height="564"></p><h4><strong>Recommended Use</strong></h4><ul><li>Used confirmation of pesticides, PCB, or other environmental samples</li><li>Robust column performance for high temperature bakeouts</li></ul><h4><strong>Recommended Applications</strong></h4><ul><li>EPA Methods</li><li>Herbicides</li><li>Pesticides</li><li>Polychlorinated Biphenyls (PCBs)</li></ul><h5><strong>Similar* To:</strong></h5><ul><li>MDN-12</li><li>DB-XLB</li><li>Rtx-XLB</li></ul><p><strong>*Not exact equivalent, selectivity may differ</strong></p><h3><strong>Phenomenex Zebron Inferno GC Columns</strong></h3><h4><strong>Overcome Traditional Obstacles</strong></h4><h5><strong>Challenge # 1: Short Fused Silica Column Lifetimes</strong></h5><p>Unlike Zebron™ Inferno™ GC columns, traditional fused silica columns are not equipped to handle prolonged exposure to<br>temperatures above 360 °C. At high temperatures, fused silica GC columns:</p><ul><li>Become inflexible and brittle</li><li>Break easily and cause costly replacement</li><li>Require more system downtime for maintenance</li><li>Can’t separate high molecular weight compounds</li></ul><h5><strong>Challenge # 2: Cumbersome Metal Column Tubing</strong></h5><p>Though metal columns have higher temperature limits than capillary columns, they are more difficult to use.</p><p><strong>Most metal columns:</strong></p><ul><li>Are inflexible and hard to use</li><li>Require special tubing cutters for installation</li><li>Are prone to leaks from expansion and contraction</li><li>Have active surfaces, giving poor peak shapes for acids and bases</li><li>Are incompatible with MS detectors</li></ul><h3><strong>How it works</strong></h3><p>Zebron™ Inferno™ columns offer enhanced performance where other columns fail. The two critical factors that allow for this are:</p><ul><li><h5><strong>Advanced ESC™ Bonding Technology</strong></h5></li></ul><p>At high temperature ranges, the stability of standard GC columns deteriorates, resulting in increased bleed. Zebron’s Engineered Self Cross-linking™ (ESC) bonding technology reinforces the stationary phase for enhanced column durability and extremely low bleed levels at high temperatures. <strong>The result:</strong> Inferno columns with the flexibility to perform for high temp oven ramps and bakeouts.</p><ul><li><strong>High Temperature Polyimide Coating</strong></li></ul><p>Standard polyimide resin pyrolyzes at temperatures above 360 °C, making the tubing unstable. Zebron Inferno columns use a temperature resistant polyimide resin that shows minimal thermal degradation, even at temperatures up to 430 °C*. This means longer column lifetime at elevated temperatures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg" alt="Phenomenex: Zebron Inferno GC Columns - How it works" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg 245w," sizes="100vw" width="978" height="564"></p><h3><strong>Features of Zebron Inferno GC Columns</strong></h3><h4><strong>Extremely Low Bleed for Rugged Performance</strong></h4><ul><li>Longer column lifetime</li><li>Better reproducibility</li><li>Maximum sensitivity</li><li>More accurate quantitation</li></ul><h4><strong>Increased Column Lifetime with Enhanced Durability</strong></h4><ul><li>Less instrument downtime</li><li>More productivity</li><li>Lower laboratory costs</li></ul><h5><strong>Standard GC Columns Cannot Withstand High Temperature</strong></h5><p>After prolonged exposure to temperatures above 360 °C, most traditional GC columns become brittle and inflexible, often spontaneously breaking in the middle of a run. Work is stopped in order to change out the column and perform system maintenance. Unlike these fragile columns, ZebronTM InfernoTM columns have a thermally resistant polyimide coating for longer column lifetime at temperatures up to 430 °C. As a result, you get less instrument downtime, and higher productivity.</p><h4><strong>Greater Performance for High Boiling Compounds</strong></h4><ul><li>Better peak shape</li><li>Improved separation</li><li>More analytes detected</li></ul><p>Zebron™ Inferno™ columns have the inertness and temperature stability needed to separate high molecular weight compounds. These high boilers require a thermally rugged column with low bleed levels. Inferno columns provide optimal separation of these analytes.</p>

Phenomenex Zebron ZB-624plus GC Columns

Zebron ZB-624PLUS is the optimal choice for the analysis of volatile compounds for Environmental, Pharmaceutical, Food, Cannabis, and Specialty Chemicals.

<h4><strong>The Next Generation of GC Inertness</strong></h4><p>Zebron ZB-624PLUS is the optimal choice for the analysis of <strong>volatile compounds</strong> for <strong>Environmental</strong>, <strong>Pharmaceutical,</strong> <strong>Food</strong>, <strong>Cannabis</strong>, and <strong>Specialty Chemicals</strong>. The superior “Plus” deactivation process enhances peak shape, improves signal-to-noise levels and increases sensitivity for qualitative and quantitative analysis of active compounds. Upgrade your existing 624 phase to Zebron ZB-624PLUS GC columns and get all the analytical benefits of proven inertness.</p><ul><li><strong>Enhanced peak shape</strong> with superior deactivation</li><li><strong>Increased sensitivity</strong> for high boiling solvents</li><li><strong>Extremely low bleed</strong> for GC-MS</li><li><strong>High temperature stability</strong> (300/320 ºC)</li></ul><h4><strong>What makes the </strong><i><strong>PLUS</strong></i><strong> in ZB-624<sub>PLUS</sub>?</strong></h4><ul><li><h5><strong>Low Bleed</strong></h5><ul><li>Engineered Self Cross-linking™ (ESC™) provides high thermal stability with maximum column temperatures up to 300/320 °C.</li></ul></li><li><h5><strong>Enhanced Inertness</strong></h5><ul><li>Proprietary superior deactivation gives great peak shape for troublesome compounds.</li></ul></li><li><h5><strong>High Selectivity</strong></h5><ul><li>A G43 phase that’s highly selective for polar, non-polar, low and high boiling solvents.</li></ul></li><li><h5><strong>Column-to-Column Reproducibility</strong></h5><ul><li>Excellent column-to-column reproducibility well suited for validated methods.</li></ul></li><li><h5><strong>Temperature Limits</strong></h5><ul><li>Push the temperature limits of traditional 624 and elute/ bake high boiling analytes at 300/320 °C.</li></ul></li><li><h5><strong>MS Certified</strong></h5><ul><li>Low bleed characteristics makes it the right choice for GC-MS.</li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_624plus_Meet_your_Zebron_ZB_624_PLUS_GC_Column_fa4f56ed66.jpg" alt="Phenomenex: Zebron-624plus - Meet your Zebron ZB-624PLUS GC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_624plus_Meet_your_Zebron_ZB_624_PLUS_GC_Column_fa4f56ed66.jpg 245w," sizes="100vw" width="1340" height="778"></p><h4><strong>Low Bleed = Better Signal-to-Noise Ratio = Lower Detection Limits</strong></h4><ul><li>The <strong>low bleed</strong> character of <strong>ZB-624PLUS™</strong> columns is the result of advances in <strong>cross-linking</strong> manufacturing processes that we established for these columns.</li><li>With ZB-624PLUS™, your GC column undergoes proprietary cross-linking process to get extensive <strong>Engineered Self Cross-linkage (ESC™)</strong>.</li></ul><h4><strong>Recommended Use</strong></h4><ul><li>Enhanced peak shape with superior deactivation and increased sensitivity for high boiling solvent, extremely low bleed for GC-MS with high temperature stability (300/320°C)</li></ul><h4><strong>Recommended Applications</strong></h4><ul><li>Cannabis</li><li>Terpenes</li><li>Residual Solvents</li><li>Volatile Amines</li><li>EPA Method 8260</li><li>EPA Method 524</li><li>EPA Method 624</li><li>Food</li><li>Flavors and Fragrances</li><li>Solvent Purity</li><li>Alcohols</li></ul><h4><strong>Upgrade to Zebron from these similar* phases:</strong></h4><p><strong>Agilent®</strong></p><ul><li>CP-Select 624 CB</li><li>DB-624UI Ultra Inert</li></ul><p><strong>Restek®</strong></p><ul><li>Rxi-624Sil MS</li></ul><p><strong>*not exact equivalent, selectivity may differ</strong></p><h3><strong>Phenomenex Zebron PLUS GC Columns</strong></h3><h4><strong>What’s in the Zebron™ PLUS?</strong></h4><ul><li>Active sites on a GC column’s surface can result in analyte adsorption and degradation, which negatively affect peak shape and response.</li><li>To reduce potential surface activity, <strong>Zebron PLUS phases</strong> (ZB-1plus, ZB-5plus, ZB-5MSplus, and ZB-624plus) are designed with a rigorous fused silica deactivation process that improves inertness and peak shapes for troublesome compounds</li><li>Zebron ZB-WAX<sub>PLUS</sub> is specially designed for <strong>100% aqueous stability</strong>, <strong>enhanced resolution</strong> and <strong>better reproducibility</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_PLUS_GC_Column_phase_522f5f8817.jpg" alt="Phenomenex: Zebron PLUS GC Column phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_PLUS_GC_Column_phase_522f5f8817.jpg 245w," sizes="100vw" width="1191" height="454"></p>

Phenomenex Zebron ZB-1701 GC Columns

Zebron ZB-1701 Provides alternate selectivity to phenyl phases with similar polarity

<p><strong>Alternate Selectivity for Mid-Polarity Analyses</strong></p><ul><li>Fast run and re equilibration times for enhanced sample throughput and productivity</li><li>Provides alternate selectivity to phenyl phases with similar polarity</li><li>Good peak shape for active analytes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1701_75b9286de0.webp" alt="Phenomenex: Zebron ZB-1701" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1701_75b9286de0.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Use</strong></h5><ul><li>Alcohols, Amines, Aromatic Hydrocarbons, Drugs, Esters, PAHs, PCBs, Pharmaceutical Intermediates, Phenols, Solvents, Steroids, TMS Sugars, Tranquilizers</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1701_c2a6145780.jpg" alt="Phenomenex: Zebron ZB-1701" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1701_c2a6145780.jpg 143w," sizes="100vw" width="634" height="694"></p><p><strong>Upgrade to Zebron from any 14 % cyanopropylphenyl / 86 % dimethylpolysiloxane phase:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-1701&nbsp;</li><li>CP-Sil 19 CB</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-1701&nbsp;</li><li>Rtx-VMS&nbsp;</li></ul><h5><strong>SGE®</strong></h5><ul><li>&nbsp;BP10&nbsp;&nbsp;</li></ul><h5><strong>Supelco®</strong></h5><ul><li>SPB®-1701&nbsp;&nbsp;</li><li>Equity®-1701&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</li></ul><h5><strong>OV®</strong></h5><ul><li>OV-1701<br>&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;</li></ul>

Phenomenex Zebron ZB-1701P GC Columns

Zebron ZB-1701P is a guaranteed column for pesticide analysis

<p><strong>Enhanced Response for DDT and Endrin</strong></p><ul><li>Specially tested to ensure response of DDT, Endrin, Endrin Aldehyde, and Endrin Ketone</li><li>Guaranteed column for pesticide analysis</li><li>EPA Method 8081 Certified</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1701_75b9286de0.webp" alt="Phenomenex: Zebron ZB-1701P" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1701_75b9286de0.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Use</strong></h5><ul><li>Aroclors, nitrogen containing pesticides, organochlorine pesticides, organophosphorous pesticides</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1701_P_c4ccee1802.jpg" alt="Phenomenex: Zebron ZB-1701P" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1701_P_c4ccee1802.jpg 138w," sizes="100vw" width="657" height="740"></p><p><strong>Upgrade to Zebron from any 14 % cyanopropylphenyl / 86 % dimethylpolysiloxane phase:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-1701&nbsp;</li><li>DB-1701P</li><li>CP-Sil 19 CB</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-1701&nbsp;</li><li>Rtx-VMS&nbsp;</li></ul><h5><strong>SGE®</strong></h5><ul><li>&nbsp;BP10&nbsp;&nbsp;</li></ul><h5><strong>Supelco®</strong></h5><ul><li>SPB®-1701&nbsp;&nbsp;</li><li>Equity®-1701&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</li></ul><h5><strong>OV®</strong></h5><ul><li>OV-1701<br>&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;</li></ul>

Phenomenex Zebron ZB-35 GC Columns

Zebron ZB-35 is an intermediate polarity column for high molecular weight analysis

<p><strong>Low Bleed, Intermediate Polarity Selectivity for GC-MS</strong></p><ul><li>Intermediate polarity column for high molecular weight analysis</li><li>Inert to minimize analyte adsorption, improve efficiency, and reproducibility</li><li>More rugged (longer column life) than other polar phases</li><li>Excellent for trace analysis with bleed-sensitive detectors (MS, FID, ECD, NPD)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1701_75b9286de0.webp" alt="Phenomenex: Zebron ZB-35" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1701_75b9286de0.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Use</strong></h5><ul><li>Amines, Aroclors, Drugs, EPA Methods (508, 608, 8081, 8141, 8151), Pesticides, Pharmaceuticals, Semi-volatiles, Steroids</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_35_dc84d61d90.jpg" alt="Phenomenex: Zebron ZB-35" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_35_dc84d61d90.jpg 174w," sizes="100vw" width="726" height="651"></p><p><strong>Upgrade to Zebron from any 35 % phenyl / 65 % dimethylpolysiloxane phase:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-35</li><li>DB-35ms</li><li>HP-35</li><li>HP-35ms&nbsp;</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-35</li><li>Rtx-35ms&nbsp;</li></ul><h5><strong>SGE®</strong></h5><ul><li>&nbsp;BP1</li><li>BPX1-SimDBPX3 BPX608</li></ul><h5><strong>Supelco®</strong></h5><ul><li>SPB®-35</li><li>MDN-35</li><li>SPB-608&nbsp;&nbsp;</li></ul><h5><strong>OV®</strong></h5><ul><li>OV-11<br>&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;</li></ul>

Phenomenex Zebron ZB-624 GC Columns

Zebron ZB-624  is widely used phase to separate volatile organic flavor and fragrance additives and residual solvents in industrial or pharmaceutical products

<p><strong>Robust Results for VOCs and Residual Solvents</strong></p><ul><li>Widely used phase to separate volatile organic flavor and fragrance additives and residual solvents in industrial or pharmaceutical products (OVIs)</li><li>Popular choice for residual solvent testing</li><li>Excellent for US EPA Methods 501.3, 502.2, 503.1, 524.2, 601, 602, 624, 8010, 8015, 8020, 8021, 8240, 8260</li><li>Specifically designed for the separation of volatile organic compounds (VOCs)</li><li>Increased temperature limit speeds run times and re-equilibration</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5_8ee06754ca.webp" alt="Phenomenex: Zebron ZB-624" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5_8ee06754ca.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Use</strong></h5><ul><li>EPA Methods (501.3, 502.2, 503.1, 524.2, 601, 602, 624, 8010, 8015, 8020, 8021, 8240, 8260), Pharmaceuticals, Residual Solvents, Volatile Organic Compounds (VOCs)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_624_971ffc45e5.jpg" alt="Phenomenex: Zebron ZB-624" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_624_971ffc45e5.jpg 163w," sizes="100vw" width="684" height="655"></p><p><strong>Upgrade to Zebron from any 6 % cyanopropylphenyl / 94 % dimethylpolysiloxane phase:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-624</li><li>DB-1301</li><li>DB-VRX</li><li>HP-VOC</li><li>CP-1301</li><li>CP-Select 624 CB</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-624</li><li>Rtx-1301</li><li>Rtx-VMS</li></ul><h5><strong>SGE®</strong></h5><ul><li>BPX624</li></ul><p><strong>Supelco®</strong></p><ul><li>SPB®-624</li><li>SPB-1301</li></ul><p><strong>OV®</strong></p><ul><li>OV-624</li></ul><h5><strong>Zebron GC Column Benefits</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_GC_Column_Benefits_de6be151ef.jpg" alt="Phenomenex: Zebron GC Column Benefits" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_GC_Column_Benefits_de6be151ef.jpg 245w," sizes="100vw" width="1297" height="742"></p><p>&nbsp;</p>

Phenomenex Zebron ZB-XLB GC Columns

Zebron ZB-XLB is an unique, low polarity si-arylene column

<p><strong>Extra Low Bleed</strong></p><ul><li>Unique, low polarity si-arylene column</li><li>Engineered specifically for use with bleed sensitive detectors such as MS</li><li>Provides alternate selectivity to standard 5-type phases</li><li>Often used for confirmation of pesticides, PCBs, or other environmental samples</li><li>Good tool for sample screening to identify unknown contaminants</li></ul><h5><strong>Recommended Use</strong></h5><ul><li>Herbicides / Insecticides, PCBs, Pesticides , Unknown Samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_XLB_24bf657d8c.jpg" alt="Phenomenex: Zebron ZB-XLB" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_XLB_24bf657d8c.jpg 145w," sizes="100vw" width="688" height="741"></p><p><strong>Upgrade to Zebron from these similar* phases:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-XLB</li><li>VF-XMS</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rxi®-XLB</li><li>Rtx®-XLB</li></ul><p><strong>Supelco®</strong></p><ul><li>MDN-12</li></ul><p><strong>*not exact equivalent, selectivity may differ</strong></p>

Products from category Mid-Polar GC Columns distributed by Phenomenex - page 1

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