Commercial and public spectral libraries provide broad coverage for LC-MS/MS identification, but they cannot contain every compound that matters to a specific laboratory. Proprietary products, metabolites, intermediates, impurities, degradation products, biomarkers, and internally characterized compounds may be highly relevant even if they are absent from standard reference collections. NIST26 allows laboratories to build their own MS/MS user libraries and integrate them directly into routine search workflows, creating a focused resource tailored to their analytical needs.<h4>Why Create a User MS/MS Library?</h4>User libraries are usually much smaller than commercial or public spectral databases, but their value is not determined by size alone.A laboratory-specific library may contain exactly the compounds most important for a particular application. These can include:<ul><li>proprietary products,</li><li>known metabolites,</li><li>synthesis intermediates,</li><li>impurities,</li><li>degradation products,</li><li>biomarkers,</li><li>compounds associated with specific industrial or environmental samples.</li></ul>Such libraries can therefore provide highly relevant identifications in pharmaceutical development, industrial R&amp;D, medical laboratories, environmental analysis, and other specialized fields. A relatively small collection of carefully characterized compounds may be more useful for a particular laboratory than a much larger general-purpose database.&nbsp;<h4>What Information Can Be Stored with Each Spectrum?</h4>Creating a useful library entry involves more than importing an MS/MS spectrum.A complete record can combine several types of information, including the experimental spectrum, chemical structure, instrumental parameters, ionization information, sample history, and additional user-defined metadata.Spectra can be transferred from vendor software into NIST Library Search, or they can be sent from the Chromatogram tab directly to the Library Search environment in NIST26. Chemical structures can be imported through the Windows clipboard from a molecular drawing program, while standard experimental parameters and sample-history information can be prepared separately and added to the entry.&nbsp;The ability to keep analytical metadata together with the spectrum is particularly important for internally generated libraries because it preserves the context in which the reference spectrum was acquired.<h4>Using Tags to Organize LC-MS/MS Metadata</h4>NIST26 supports a range of structured tags for LC-MS/MS spectra.These can describe parameters such as:<ul><li>spectrum type,</li><li>compound type,</li><li>ion name,</li><li>precursor type,</li><li>precursor m/z,</li><li>collision energy,</li><li>instrument type,</li><li>sample identifier,</li><li>ionization mode,</li><li>ion polarity,</li><li>collision gas,</li><li>pressure,</li><li>mass range,</li><li>maximum intensity,</li><li>cone voltage,</li><li>charge,</li><li>molecular weight,</li><li>known impurities,</li><li>related CAS numbers,</li><li>peptide sequence and peptide modifications.</li></ul>This provides a convenient way to attach searchable and standardized experimental information to spectra rather than leaving all metadata in an unstructured comment field.&nbsp;Tags can be entered individually through the Additional Info section in the Librarian interface, but when many spectra are being added, a grouped approach can be considerably faster.&nbsp;<h4>Adding Groups of Tags Efficiently</h4>For repetitive workflows, metadata can be prepared in advance and pasted into NIST26 as a group.The presentation recommends maintaining frequently used tag sets in a simple text document, then copying them into the synonym field and using Add to List to convert them into structured information.This is especially useful when the vendor software already transfers some parameters automatically. Users can then add only the missing information, such as ion adduct or polarity, rather than duplicating metadata already imported with the spectrum.&nbsp;Care is required because entering information that is already present may create duplicate metadata.This workflow can substantially accelerate library creation when many spectra share the same instrument configuration or acquisition parameters.<h4>Preserving Sample History</h4>A spectral match is more useful when the origin of the reference spectrum is known.NIST26 therefore allows detailed sample information to be stored in the comment field. For batches of related samples, the text can be prepared in Notepad or another editor and pasted into multiple records.Examples of useful information include sampling location, sampling date and time, analyst name, organization, internal sample identifier, project information, or other relevant context.&nbsp;For environmental, clinical, pharmaceutical, and industrial laboratories, this information can become an important part of data traceability.<h4>Adding Molecular Structures</h4>A user library becomes considerably more useful when spectra are linked to molecular structures.Structures can be created from scratch in a chemical drawing program. Alternatively, a similar structure can be located in NIST Search and sent to the default structure editor, where it can be modified as needed.Once completed, the structure can be copied to the Windows clipboard and inserted into the NIST Librarian spectrum-information window. NIST26 can then calculate the molecular formula and molecular weight from the imported structure.&nbsp;This linkage between spectrum and structure supports more informative library displays and structure-based searching.<h4>Creating Custom User-Defined Tags</h4>In addition to the standard LC-MS/MS tags, laboratories can define their own metadata fields.Examples shown include identifiers such as:<ul><li>chemist,</li><li>laboratory notebook number,</li><li>analyst.</li></ul>The information can be prepared in a text editor and added using the appropriate tag syntax. Once configured in the Comment Field Display settings, these values are displayed as separate structured lines rather than remaining embedded in the general comments.&nbsp;This flexibility makes the library more suitable for laboratories with established internal documentation systems or quality-management requirements.NIST can also automatically add information such as the InChIKey and an estimated retention index when appropriate structural information is available.&nbsp;<h4>High-Resolution and Low-Resolution Libraries Should Be Kept Separate</h4>User libraries do not have to contain only high-resolution spectra.Low-resolution MS/MS spectra may also be valuable, including spectra generated by:<ul><li>in-source collision on single-quadrupole instruments,</li><li>triple-quadrupole MS/MS,</li><li>other nominal-mass workflows.</li></ul>The recommendation is to maintain separate high-resolution and low-resolution user libraries, because the appropriate search strategies and handling can differ.&nbsp;Low-resolution spectra can still generate strong library-search results and may be particularly useful when laboratories want to preserve historical data or incorporate spectra from multiple instrument types.<h4>Important Software Considerations When Importing Spectra</h4>The presentation also highlights several practical software issues that users should be aware of.One concerns the Intensity Threshold setting in the Spectrum Import Options window. When an intensity threshold is applied during import from vendor software, high-resolution spectral values may be converted to nominal mass rather than preserving the expected decimal precision.For laboratories building high-resolution user libraries, this is an important detail because accurate-mass information is one of the main advantages of HRMS data.Another issue affects some library-search modes after high-resolution spectra are added to a user library.<h4>HiRes No Precursor and Hybrid Search Limitations</h4>After adding high-resolution spectra, certain HiRes NoPrecursor and Similarity Hybrid MS/MS searches may not function correctly.A workaround involves exporting the affected library using Lib2NIST into MSP ASCII files together with the associated molecular structure files, then importing the data back into NIST library format.&nbsp;The presentation specifically warns against attempting the problematic MS/MS Hybrid Search under these conditions because it may cause the software to crash.An EI Normal search can mimic some of the functionality of HiRes No Precursor and may work effectively when high- and low-resolution libraries are searched together. HiRes NoPrecursor can also operate with presearch disabled, although this can make searching considerably slower.&nbsp;<h4>Display Issues in the Chromatogram Window</h4>Another software behavior concerns how spectral values are displayed in the Chromatogram tab.Values from Spectrum Import Options are reflected in the head-to-head spectral display. If settings are adjusted for low-resolution spectra, the displayed masses may appear truncated.Importantly, this visual behavior does not affect the results in the accurate-mass deconvolution and library-searching table; it affects only the spectral display at the top of the Chromatogram window.&nbsp;Distinguishing display behavior from actual processing results is important to avoid misinterpreting valid search results as corrupted data.<h4>Updating the Library After Adding New Entries</h4>User-library maintenance does not end when a spectrum has been added.Whenever a new entry containing a molecular structure is introduced, the structure index should be updated so that the new compound can be included properly in structure searching.The InChIKey appears to update with the structure, but if duplicate records are not being handled correctly, the InChIKey index may also need to be rebuilt.&nbsp;If a new library folder is added to the NIST26 MSSEARCH directory while the software is already running, users should also update the library list so that the newly added collection becomes available without restarting the entire environment.&nbsp;<h4>Using Lib2NIST to Repair High-Resolution User Libraries</h4>The Lib2NIST utility plays an important role in resolving some high-resolution library-search issues.The workflow involves exporting the problematic high-resolution library to an MSP file together with its molecular structure files and then re-importing the data into NIST library format.The corrected library can then be placed back into the NIST26 MSSEARCH directory. It is advisable to preserve the original library under a different name until the repaired version has been tested successfully.&nbsp;The presentation also recommends closing NIST Search while performing Lib2NIST conversions.<h4>Small Libraries Can Deliver High Analytical Value</h4>The central message is straightforward: a user library does not need to contain hundreds of thousands of compounds to be useful.A carefully curated collection of spectra from compounds that repeatedly appear in a laboratory's own samples can dramatically improve identification relevance. This is especially valuable for compounds that are proprietary, newly synthesized, application-specific, or otherwise poorly represented in public and commercial databases.By combining MS/MS spectra with structures, acquisition metadata, sample history, tags, and internally defined information, NIST26 can become more than a search engine for commercial databases. It can serve as a laboratory-specific knowledge base built around compounds that genuinely matter to the user's work.For specialized LC-MS/MS workflows, that may be one of the most valuable ways to extend the capabilities of spectral library searching.

Creating MS/MS User Libraries in NIST26

Wiley announces the expansion of its spectral libraries across IR and Raman, adding empirical and computed reference data spanning forensics, pigments and dyes, mineralogy, materials, polymers, and pharmaceuticals. This release adds over a thousand new empirical reference spectra, plus more than 429,000 new SmartSpectra records, across dozens of updated and new databases. This brings the combined collections to over 1.4 million IR spectra and 230,000 Raman spectra.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Wiley_continues_to_expand_its_IR_Raman_and_Smart_Spectra_spectral_libraries_806b34cdc1.jpg" alt="Wiley: Wiley continues to expand its IR, Raman, and SmartSpectra spectral libraries" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Wiley_continues_to_expand_its_IR_Raman_and_Smart_Spectra_spectral_libraries_806b34cdc1.jpg 245w," sizes="100vw" width="1600" height="838">“Scientific discoveries and business decisions depend on trustworthy analytical results,” said Graeme Whitley, senior director, data science solutions at Wiley. “The value of a spectral library expansion is not simply that it adds more records. It gives analysts more relevant reference candidates when they encounter unfamiliar materials, whether they are verifying a polymer, investigating a pharmaceutical sample, examining a mineral, or screening a forensic specimen.”<h4>Highlights include:</h4><ul><li>Forensics: A new Raman forensics database adds reference spectra for colorant and dye identification on textiles, drawn from real fiber-evidence research, useful for investigators working on dye and textile analysis.</li><li>Pigments and dyes: New Raman and IR data significantly broadens pigment and dye coverage, including historic artists' pigments, supporting art conservation and cultural-heritage authentication work.</li><li>Minerals: New Raman and IR data extends coverage to minerals from different localities and Martian mineral analogs. This release includes new Raman additions from our ongoing collaboration with the American Museum of Natural History, now cross-referenced with DANA Classification Numbers and Mindat IDs, giving mineralogists and gemologists an easier way to identify entries against established references.</li><li>Materials and semiconductors: New Raman and IR data covers crystal growth relevant to battery materials, rare-earth-doped magnetic crystals, and flexible and 2D semiconductor materials, useful for researchers in battery, magnetic materials, and electronics work.</li><li>Polymers: New Raman and IR data spans 3D-printed, MOF-integrated microlattices, environmental plastic aging and recycled PET materials, and food packaging plastics. New Raman reference spectra also cover more than two dozen synthetic polymer types for identifying microplastics in environmental samples.</li><li>Clinical and pharma: New Raman and IR data covers antibiotic classes, including beta-lactams, and the WHO's essential antibiotic list, plus bone mineralization studies in clinical models. A new IR dataset also adds spectra for common over-the-counter medications, including aspirin, ibuprofen, and paracetamol, helping quality control teams confirm formulations faster.</li><li>SmartSpectra: Our SmartSpectra IR, Raman, and vapor-phase IR collections continue to grow, adding 243,000+ IR, 67,000+ Raman, and 119,000+ vapor-phase IR computed reference spectra to support broad, high-throughput identification.</li></ul><blockquote><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/spectral-databases/">Learn more about Wiley Spectral Databases</a><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/how-to-update-knowitall-databases/">Existing subscribers can find guidance on updating their databases here</a></blockquote><h4>Wiley Spectral Databases</h4>Wiley's spectral databases are consistently evolving to meet today's research demands, covering multiple spectral techniques across a wide range of applications including polymers, materials, environmental, forensics, toxicology, pharmaceutical, biotech, automotive, aerospace, food, cosmetics, and more. Built on rigorous quality protocols, Wiley delivers industry-leading spectral data and intelligent tools to accelerate analysis and deliver deeper insights — making it the authoritative, trusted source that analytical labs worldwide rely on.&nbsp;

Wiley continues to expand its IR, Raman, and SmartSpectra spectral libraries

Per- and polyfluoroalkyl substances (PFAS)&nbsp;analysis is no longer&nbsp;defined by&nbsp;a fixed list of known compounds.As regulatory frameworks evolve and scientific understanding deepens, laboratories are being asked to go beyond routine testing. In addition to quantifying known PFAS, they must also investigate unknown, unexpected, and emerging compounds that fall outside predefined target lists.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_509ce49fc2.webp" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_509ce49fc2.webp 245w," sizes="100vw" width="1024" height="526">This shift introduces a fundamental analytical challenge.Targeted quantitation provides robust and validated concentration data for known PFAS. However, by design, it only answers questions about compounds already included in the method. In contrast, non-targeted analysis,&nbsp;using high-resolution accurate-mass&nbsp;(HRAM)&nbsp;data,&nbsp;enables scientists to explore the broader chemical landscape, revealing&nbsp;additional&nbsp;PFAS&nbsp;that were not included in a targeted quantitation method.For PFAS, where thousands of structurally related compounds and transformation products may be present, neither approach&nbsp;alone provides a complete picture.The&nbsp;real challenge&nbsp;lies in connecting these workflows. Discovery findings must be translated into actionable results that can be reviewed, followed up, and reported within a controlled analytical framework.In a previous&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7167">blog post</a>, we introduced how <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759">Thermo Scientific Compound Discoverer software </a>and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/76">Thermo Scientific Chromeleon Chromatography Data System&nbsp;(CDS)&nbsp;</a>connect discovery and routine workflows.&nbsp;Here, we explore what that connection looks like in practice using PFAS analysis as an example.<h3>Why PFAS&nbsp;requires&nbsp;more than one analytical question</h3>PFAS&nbsp;represent a&nbsp;large&nbsp;and diverse class of compounds,&nbsp;with&nbsp;some&nbsp;estimates exceeding&nbsp;40,000 individual substances. Regulatory methods typically focus on a limited subset, yet environmental and biological samples often&nbsp;contain&nbsp;many more&nbsp;PFAS, including precursors and transformation products.This creates two&nbsp;essential and&nbsp;complementary analytical questions:How much of these known PFAS are present?andWhat other PFAS-related compounds might be present?A complete workflow needs to support both&nbsp;questions.<h3>Starting with targeted quantitation</h3>For known PFAS, targeted quantitation&nbsp;remains&nbsp;the&nbsp;backbone of routine analysis.Chromeleon CDS provides&nbsp;a&nbsp;controlled environment for&nbsp;data acquisition, processing, calibration, and reporting. In a PFAS workflow,&nbsp;this supports routine quantitation of predefined targets using laboratory-specific method and review criteria.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_2_7ff8eda8fe.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 1. Targeted quantitation of known PFAS in Chromeleon CDS.&nbsp;This example is&nbsp;from the analysis of PFAS using a&nbsp;Thermo Scientific&nbsp;Orbitrap Exploris&nbsp;240. Here&nbsp;we are showing&nbsp;the&nbsp;calibration curve (right) and extracted&nbsp;ion chromatogram&nbsp;(left)&nbsp;of 7:3 FTCA&nbsp;at 6 ng/L.&nbsp;The bottom&nbsp;half&nbsp;shows the processing method that&nbsp;contains&nbsp;many target PFAS and&nbsp;isotopically-labelled&nbsp;internal standards." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_2_7ff8eda8fe.jpg 208w," sizes="100vw" width="794" height="596">This targeted workflow is essential, but it is only part of the story. A sample may contain PFAS-related compounds not included in the original method.<h3>Looking beyond known PFAS</h3>Once HRAM data have been acquired, the same dataset can be explored beyond the target list using Compound Discoverer software. This enables comprehensive analysis of complex datasets, including:<ul><li>Detection and alignment of unknown features</li><li>Statistical comparison across samples and blanks</li><li>Spectral library searching and database annotation</li><li>Evaluation of isotope patterns, fragmentation, and homologous series</li></ul>For PFAS, this is particularly valuable. The software can leverage characteristic properties such as negative mass defect, CF₂-based homologous series, and diagnostic fragment ions to prioritize PFAS-related candidates and assign confidence to putative identifications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_3_e523e1cdc9.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 2. Discovery of additional PFAS-related components in Compound Discoverer software. Here we show an example of using PFAS fragment libraries, one of many PFAS-specific resources available within Compound Discoverer software, to annotate fragments within acquired MS2 spectra of two unknown compounds and then identify them as PFAS that were outside the targeted quantitation method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_3_e523e1cdc9.jpg 233w," sizes="100vw" width="1520" height="1016">The goal of discovery is not simply to generate more data. It is to reduce complexity and identify the most relevant candidates for further investigation with a defined level of confidence.<h3>From discovery to follow-up</h3>One of the most important questions in non-targeted analysis is what happens after candidate identification.Discovery workflows can identify interesting candidates, but those candidates need to move into a practical follow-up process. Scientists may want to monitor them across additional samples, estimate relative or semi-quantitative abundance, trend their presence over time, or include them in a reviewable report.This is where the connection between Compound Discoverer software and Chromeleon CDS becomes especially valuable.After PFAS candidates have been prioritized in Compound Discoverer software, selected components can be transferred back into Chromeleon CDS and incorporated into the Chromeleon processing methods for follow-up analysis. This allows scientists to evaluate these compounds within the same controlled environment used for targeted quantitation.For PFAS analysis, this may support semi-quantitative follow-up for compounds where authentic standards are not yet available. While fully quantitative results require appropriate standards and validated methods, semi-quantitative workflows can provide useful estimates or relative comparisons to help prioritize further investigation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_4_8ee8ebc2df.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 3. Prioritized PFAS candidates brought back into Chromeleon CDS for follow-up. Prioritized PFAS candidates from Compound Discoverer software can be brought back into Chromeleon CDS for follow-up review, semi-quantitative evaluation, and controlled reporting." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_4_8ee8ebc2df.jpg 245w," sizes="100vw" width="1512" height="751">This is the key workflow advantage: discovery does not stop at identification. It can feed directly into a practical analytical process.<h3>Why this matters</h3>PFAS analysis increasingly requires laboratories to balance routine testing with exploratory investigation.A connected workflow between Chromeleon CDS and Compound Discoverer software supports this by:<ul><li>Combining targeted quantitation and non-targeted discovery to characterize samples more completely</li><li>Reducing manual data transfer and workflow fragmentation</li><li>Enabling discovery results to be reviewed and reported within a controlled CDS environment</li><li>Supporting investigation of emerging PFAS investigations without immediate method redevelopment</li><li>Bridging research-driven insights with routine analytical workflows</li></ul>This reflects a broader shift in analytical science, where the value lies not only in generating data, but in connecting workflows and turning data into actionable information.<h3>Moving PFAS analysis forward</h3>PFAS analysis is evolving from a fixed target list toward a more comprehensive understanding of complex chemical mixtures.Laboratories must quantify known compounds while remaining prepared to identify and evaluate what lies beyond them.By connecting Compound Discoverer software with Chromeleon CDS, laboratories can move seamlessly from targeted quantitation to discovery and into structured follow-up. The result is a more complete view of PFAS contamination and a more efficient path from data to insight.<h3>Learn more</h3>Explore how <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759">Compound Discoverer software</a> and<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/76"> Chromeleon CDS </a>can help connect targeted quantitation and non-targeted discovery workflows for <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/environmental/environmental-contaminant-analysis/emerging-contaminants/pfas-testing.html">PFAS analysis</a>.<blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #Chromeleon #CompoundDiscoverer #Environmental</blockquote>

PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow

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The Kinetex C8 HPLC column is a core-shell-based particle with a bonded silica phase ideal for the analysis of very hydrophobic compounds. 

Hydrophilic Polar Selectivity and Performance!The Kinetex C8 HPLC column is a core-shell-based particle with a bonded silica phase ideal for the analysis of very hydrophobic compounds. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run-time, increase sensitivity, and even provide greater resolution with much higher efﬁciency levels.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems*</li><li>Balancing Performance &amp; Retention</li><li>USP:L7</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp" alt="Phenomenex: Kinetex Core-Shell C8 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 8 (effective), 8 (effective) %</li><li>Surface Area (m²/g): 200</li><li>Particle Size (μm): 1.7, 2.6, 5</li><li>Recommended Use: Minimized hydrophobic retention for highly hydrophobic compounds</li></ul><h4>Balancing Speed and Retention</h4>The Kinetex core-shell C8 provides the same Kinetex performance but with a little less hydrophobic retention in comparison to the Kinetex C18 column. 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This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Luna C8(2) HPLC Columns

The C8 column provides less hydrophobic retention than our C18 columns, but the density of the ligand bonding creates more steric based selectivity.

The C8 column provides less hydrophobic retention than our C18 columns, but the density of the ligand bonding creates more steric based selectivity.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>C8(2) Ultra-pure, metal-free silica (99.99% purity) (USP:L7)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp" alt="Phenomenex: Luna C8(2) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 13.5, 13.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the back bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Common Applications:</h4><ul><li>Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine</li></ul><blockquote>Stationary phase Luna C8(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna C8(2) HPLC Microbore, Minibore, MidBore, Analytical and Semi-preparative Columns are available with these parameters:</h5>Microbore Columns<ul><li>Column length (mm): 50, 150</li><li>Column ID (mm): 1</li><li>Particle size (µm): 3, 5</li></ul>Minibore Columns<ul><li>Column length (mm): 30, 50, 100, 150, 250</li><li>Column ID (mm): 2</li><li>Particle size (µm): 3, 5</li></ul>MidBore Columns<ul><li>Column length (mm): 30, 50, 150, 250</li><li>Column ID (mm): 3</li><li>Particle size (µm): 3, 5</li></ul>Analytical Columns<ul><li>Column length (mm): 30, 50, 75, 100, 150, 250</li><li>Column ID (mm): 4.6</li><li>Particle size (µm): 3, 5</li></ul>Semi-Prep Columns<ul><li>Column length (mm): 250</li><li>Column ID (mm): 10</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Intact XB-C8 LC columns

Biozen Intact XB-C8 column contains skillfully manufactured large pore core shell particles that provide narrower, taller peaks in conjunction with higher resolution.

Impurity profiling and characterization of intact biologic fragments is a challenging undertaking because of the need to identify very small differences between variants. Both bioZen Intact columns contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target HC/LC, Fc/Fab, or isoforms.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Biozen_Intact_XB_C8_HPLC_Column_c2159e0847.webp" alt="Phenomenex: Biozen Intact XB-C8 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Biozen_Intact_XB_C8_HPLC_Column_c2159e0847.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Separation Mode: Reversed Phase</li><li>Particle size: 3.6</li><li>Recommended Use: Fast separation of intact and subunit separation with hydrophobic selectivity</li></ul><h3>Phenomenex Biozen Biologics LC Columns</h3>With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:<ul><li>Charge Variant Analysis</li><li>Aggregate Analysis</li><li>Peptide Mapping</li><li>Intact Mass Analysis</li><li>Intact and Subunit Analysis</li><li>Glycan Analysis</li><li>Peptide Quantitation</li><li>Drug Antibody Ratio (DAR)</li><li>Oligonucleotide Characterization</li></ul><h3>Biocompatible Flow Path with BioTi™ Hardware</h3>Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Bio_Ti_Hardware_991fb53395.jpg" alt="Phenomenex: BioTi Hardware" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Bio_Ti_Hardware_991fb53395.jpg 245w," sizes="100vw" width="1378" height="426"><h3>4 Advanced Particle Platforms</h3>All four of the Biozen particle platforms were individually designed and built by Phenomenex to take advantage of integral levels of performance, ruggedness, and reproducibility for protein characterization applications. Individually, each platform differs in the proprietary processing techniques used to control particle size and morphology.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg" alt="Phenomenex: Advanced Particle Platforms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Advanced_Particle_Platforms_a0959aca03.jpg 245w," sizes="100vw" width="1439" height="868">

Phenomenex Luna C8(2) HPLC Preparative Columns

The C8 column provides less hydrophobic retention than our C18 columns, but the density of the ligand bonding creates more steric based selectivity.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>C8(2) Ultra-pure, metal-free silica (99.99% purity) (USP:L7)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp" alt="Phenomenex: Luna C8(2) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_C8_2_HPLC_Column_6479cab908.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 13.5, 13.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the back bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Common Applications:</h4><ul><li>Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine</li></ul><blockquote>Stationary phase Luna C8(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna C8(2) HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5, 10</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Kinetex Core-Shell C8 Preparative HPLC Columns

Hydrophilic Polar Selectivity and Performance!The Kinetex C8 HPLC column is a core-shell-based particle with a bonded silica phase ideal for the analysis of very hydrophobic compounds. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run-time, increase sensitivity, and even provide greater resolution with much higher efﬁciency levels.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems*</li><li>Balancing Performance &amp; Retention</li><li>USP:L7</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp" alt="Phenomenex: Kinetex Core-Shell C8 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Core_Shell_C8_HPLC_Column_eb663e39df.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 8 (effective), 8 (effective) %</li><li>Surface Area (m²/g): 200</li><li>Particle Size (μm): 5</li><li>Recommended Use: Minimized hydrophobic retention for highly hydrophobic compounds</li></ul><h4>Balancing Speed and Retention</h4>The Kinetex core-shell C8 provides the same Kinetex performance but with a little less hydrophobic retention in comparison to the Kinetex C18 column. This C8 column is ideal for extremely hydrophobic compounds that might be too strongly retained on a traditional C18 phase column.<blockquote>Stationary phase Kinetex Core-Shell C8 is provided in these column types: analytical and preparative</blockquote>Axia Packed Preparative Columns<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Gemini C8(3) HPLC Columns

Gemini C8(3) purification media is optimized for human insulin and its analogues, offering exceptional mechanical strength, high packing efficiency, and enhanced resistance to caustic regeneration conditions.

<h4>Optimized Purification Media for Human Insulin and It's Analogues</h4><ul><li>Enhanced Robustness Against Caustic Regeneration Conditions</li><li>High Packing Efficiencies</li><li>Incredible Mechanical Strength</li></ul>Typically insulin purification methodologies include vital steps for column efficiency tests, equilibration, loading, and multiple caustic washes. In particular, the high pH caustic washes are very important for the removal aggregate build up, however these wash steps typical diminish column lifetime quite substantially. With the improved chemical robustness and performance of Gemini C8(3) customers can now switch their focus to higher yields and purities and minimize operational down-time due to less frequent need to replace contaminated media.<h4>QC Tested to ensure consistent selectivity for Insulin and Impurity</h4>In conjunction with a media designed specifically for insulin purification, the Quality team at Phenomenex strives to ensure high reproducibility of Gemini 10µm C8(3) through numerous quality control steps throughout the manufacturing process, including a fit for purpose human insulin QC test. This elevated level of focus on quality ultimately results in a model product for large scale insulin purification.<h5>Key features:</h5><ul><li>Carbon Load: -</li><li>Surface Area (m²/g): -</li><li>Particle Size: 10</li><li>Recommended Use: Separation of very hydrophobic compounds</li></ul><h3>Gemini HPLC Columns</h3><h4>Twin-NX technology HPLC columns. Extend your column lifetime</h4>Gemini columns are rugged reversed-phase HPLC and PREP LC columns that offer extended lifetime at pH 1-12 conditions and excellent stability for reproducible, high-efficiency separations. The Gemini family contains a variety of useful alkyl and phenyl phases bonded to fully porous organo-silica particles.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_936267c483.jpg" alt="Phenomenex: Gemini Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_936267c483.jpg 134w," sizes="100vw" width="349" height="405"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg" alt="Phenomenex: Gemini-NX Twin Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Gemini_HPLC_Columns_2_e67e00cc54.jpg 134w," sizes="100vw" width="349" height="405">Each Gemini column is supplied with a Certificate of Quality Assurance (CQA) indicating testing conditions, operating parameters, and column details.

Products from category C8 HPLC columns distributed by Phenomenex - page 1

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