Thermo Scientific SurePac™ Protein RP MDi™ is a bioinert polymeric reversed-phase column engineered for robust HPLC, UHPLC, and LC-MS analysis of intact proteins, monoclonal antibodies (mAbs), antibody subunits, and antibody-drug conjugates (ADCs). Its bioinert, metal-free flow path minimizes unwanted interactions between proteins and column hardware, supporting high recovery, reproducible peak shape, and consistent separation performance.
The column uses monodisperse, supermacroporous Poly-DVB polymeric particles designed for efficient separation of large biomolecules. A broad pH range of 2–12 and operating temperatures up to 90 °C provide the flexibility required for demanding biopharmaceutical workflows and elevated-temperature LC-MS methods.
Poly-DVB Stationary Phase
SurePac Protein RP MDi uses a polymeric Poly-DVB stationary phase that provides broad chemical and thermal stability.
The material supports methods involving:
- elevated temperatures,
- high organic content,
- rapid gradients,
- a broad pH range.
This flexibility is particularly valuable for optimization of intact-protein reversed-phase separations.
Supermacroporous Architecture
The proprietary supermacroporous particles are designed to provide efficient mass transfer for large biomolecules.
Representative analytes include:
- intact proteins,
- large therapeutic proteins,
- monoclonal antibodies,
- mAb subunits,
- ADCs,
- protein variants and isoforms.
Bioinert Hardware
Protein interactions with metallic chromatographic surfaces can contribute to adsorption, analyte loss, or altered peak shape.
The bioinert design helps:
- minimize nonspecific adsorption,
- improve analyte recovery,
- enhance peak symmetry,
- improve reproducibility,
- reduce variability for sensitive therapeutic proteins.
LC-MS Optimization
SurePac Protein RP MDi is designed for LC-MS protein workflows and is compatible with MS-friendly mobile phases.
Representative applications include:
- intact-mass analysis,
- protein-variant characterization,
- mAb analysis,
- ADC characterization,
- biopharmaceutical development and quality control.
High-Temperature Operation
The column supports temperatures up to 90 °C, providing additional flexibility for reversed-phase protein separations.
Elevated temperatures can support:
- improved mass transfer,
- reduced mobile-phase viscosity,
- optimized selectivity,
- improved peak shape,
- faster chromatographic methods.
Technical Specifications
- Column Format: Analytical
- Separation Mode: Reversed phase
- Application: Intact proteins, mAbs, antibody subunits, and ADCs
- Stationary Phase: Poly-DVB
- Packing Material: Polymeric
- Pore Structure: Proprietary supermacroporous
- Particle Size: 2.5 µm
- Recommended Flow Rate: 0.2–0.6 mL/min
- Maximum Pressure: 448 bar / 6500 psi
- Temperature Range: 5–90 °C
- pH Range: 2–12
- Hardware: Bioinert, metal-free flow path
- Compatible Techniques: HPLC, UHPLC, LC-UV, LC-MS
Typical Applications
- Intact proteins
- Monoclonal antibodies
- Antibody subunits
- Antibody-drug conjugates
- Therapeutic proteins
- Protein variants
- Protein isoforms
- Biopharmaceutical characterization
- Protein LC-MS
- Method development
- R&D
- QA/QC
