Thermo Scientific SurePac™ Oligo RP MDi™ is a bioinert polymeric reversed-phase column purpose-built for HPLC and LC-MS analysis of oligonucleotides and nucleic acids. It combines monodisperse supermacroporous Poly-DVB packing with a metal-free flow path to minimize unwanted interactions between oligonucleotides and metal surfaces. This supports high recovery, clean peak shapes, and reproducible separations for short, long, modified, and analytically challenging sequences.
The column is optimized for ion-pair reversed-phase LC-MS and is compatible with commonly used ion-pairing systems such as HFIP/TEA. Its robust polymeric stationary phase supports operation across pH 2–12 and temperatures up to 90 °C, enabling demanding gradients, closely related impurity separations, and workflows spanning R&D through routine QC.
Poly-DVB Stationary Phase
SurePac Oligo RP MDi uses a Poly-DVB polymeric stationary phase. Compared with conventional silica-based materials, the polymeric packing provides broad pH compatibility and robust operation at elevated temperatures.
This is particularly valuable for oligonucleotide methods requiring:
- elevated pH,
- increased temperature,
- high organic content,
- rapid gradients,
- repeated routine analyses.
Supermacroporous Architecture
The proprietary supermacroporous polymeric packing is designed to provide efficient mass transfer for larger biomolecules.
The column supports separation of:
- short oligonucleotides,
- therapeutic oligonucleotides,
- long DNA/RNA sequences,
- chemically modified oligonucleotides,
- ultra-large oligonucleotides and intact nucleic acids.
Bioinert Hardware
Oligonucleotide phosphate groups can interact with metallic surfaces within conventional chromatographic flow paths.
The bioinert design helps:
- minimize analyte adsorption,
- improve recovery,
- reduce peak tailing,
- improve reproducibility,
- limit loss of low-level or sensitive sequences.
This makes the column particularly suitable for bioinert UHPLC and LC-MS workflows.
Ion-Pair RP LC-MS
SurePac Oligo RP MDi is optimized for ion-pair reversed-phase chromatography.
Compatibility with ion-pairing reagents such as HFIP/TEA supports LC-MS workflows requiring:
- reproducible retention,
- high chromatographic selectivity,
- efficient MS detection,
- resolution of closely related sequences,
- quantitation of main components and impurities.
Impurity Profiling
The column provides the resolving power required for structurally similar oligonucleotide species, including:
- n−1 / n−2 truncation impurities,
- extended sequences,
- sequence variants,
- synthesis by-products,
- degradation products,
- chemically modified species.
Technical Specifications
- Column Format: Analytical
- Separation Mode: Reversed phase
- Application: Oligonucleotide and nucleic acid analysis
- Stationary Phase: Poly-DVB
- Packing Material: Polymeric
- Pore Structure: Proprietary supermacroporous
- Particle Size: 2.5 µm
- Recommended Flow Rate: 0.2–0.6 mL/min
- Maximum Pressure: 448 bar / 6500 psi
- Temperature Range: 5–90 °C
- pH Range: 2–12
- Hardware: Bioinert / metal-free flow path
- Compatible Techniques: HPLC, UHPLC, LC-UV, LC-MS
Typical Applications
- Therapeutic oligonucleotides
- DNA oligonucleotides
- RNA oligonucleotides
- Chemically modified oligonucleotides
- Long oligonucleotides
- Nucleic acids
- Oligonucleotide impurity profiling
- Truncation impurity analysis
- LC-MS characterization
- LC-UV analysis
- Method development
- R&D
- Biopharmaceutical analysis
- QA/QC
