ProtiFi S-Trap™ Turbo™ 96-well Mini Plate is a high-throughput 96-well sample-preparation plate designed for rapid proteomic workflows prior to LC-MS/MS. It processes 10–250 µg of total protein per well while retaining the key benefits of S-Trap™ technology, including robust SDS-based protein solubilization, contaminant removal, and enzymatic digestion directly within the matrix. Unlike the standard S-Trap™ format, Turbo™ is optimized for low-volume elution, eliminating the need to concentrate peptides prior to LC-MS/MS.
S-Trap™ Turbo™ uses a polymeric material with more than 100× greater surface capture density than the original design. The increased binding efficiency allows less capture matrix to be used, resulting in smaller elution volumes and a single low-volume elution step. Total processing time, including digestion, is reduced from more than five hours with the standard format to approximately 2.5 hours.
S-Trap™ Turbo™ Technology
S-Trap™ Turbo™ advances the original S-Trap™ design by using a polymeric capture material with substantially increased binding-site density. This allows proteins to be efficiently trapped using less matrix while retaining SDS-compatible cleanup and direct digestion.
The optimized design enables:
- efficient capture with less matrix,
- reduced elution volume,
- replacement of multiple elution steps with a single low-volume elution,
- elimination of lyophilization or SpeedVac concentration before LC-MS/MS,
- fewer sample-handling steps,
- reduced potential peptide loss,
- increased sample-processing throughput.
According to ProtiFi, the shortened workflow provides analytical performance comparable to or better than the standard S-Trap™ format.
Standard Workflow
- Protein solubilization and denaturation with SDS
- Optional DNA shearing or nuclease treatment
- Reduction with TCEP
- Alkylation with MMTS
- Acidification to pH ≤1
- Addition of methanolic Binding/Wash Solution
- Transfer to the S-Trap™ Turbo™ 96-well Mini Plate
- Protein trapping
- Washing and contaminant removal
- Protease addition
- Digestion for 2 h at 47 °C
- Single low-volume elution
- Peptides are ready for downstream LC-MS/MS analysis.
Technical Specifications
- Samples per Plate: up to 96
- Total Protein Amount: 10–250 µg per well
- Approximate Well Capacity: 450 µL
- Standard Centrifugation: 2,000 × g for 2 min
- Alternative Flow Methods: vacuum or positive pressure when wells exhibit similar flow behavior
- Washing: 200 µL Binding/Wash Solution, 3 times
- Protease: application dependent, e.g. trypsin or Trypsin/Lys-C
- Protease-to-Protein Ratio: 1:10 to 1:100 (w/w)
- Digestion Solution Volume: 30 µL
- Digestion: 2 h at 47 °C
- Elution: 20 µL Digestion Solution
- Final Elution Steps: 1
- Post-Elution Concentration: not required
- Downstream Analysis: LC-MS / LC-MS/MS
- Intended Use: Research Use Only – not for diagnostic procedures.
Workflow Reagents
The protocol uses:
- 2× Lysis Solution: 10% SDS, 100 mM TEAB
- Reductant: 120 mM TCEP
- Alkylator: 500 mM MMTS in IPA
- Acidifier: 55% phosphoric acid
- Binding/Wash Solution: 100 mM TEAB in 90% methanol
- Digestion Solution: 50 mM TEAB, pH 8.0
- user-selected protease such as trypsin or Trypsin/Lys-C
These reagents are not included with the standalone S-Trap™ Turbo™ 96-well Mini Plate.
Available Pack Sizes
- PN P003-96MNT-0001PL – 1 S-Trap™ Turbo™ 96-well Mini Plate
- PN P003-96MNT-0003PL – 3 S-Trap™ Turbo™ 96-well Mini Plates
- PN P003-96MNT-0010PL – 10 S-Trap™ Turbo™ 96-well Mini Plates
Typical Applications
- High-throughput bottom-up proteomics
- Discovery proteomics
- Large cohort studies
- Comparative proteomic screening
- LC-MS/MS sample preparation
- SDS-compatible proteomic workflows
- Cell and tissue lysates
- Complex biological samples
- Membrane and poorly soluble proteins
- Label-free proteomics
- TMT and other isobaric-labeling workflows
- Automated 96-well sample preparation
