ProtiFi S-Trap 96-well Micro Plate for Proteomics Sample Preparation

ProtiFi S-Trap™ 96-well Micro Plate is a 96-well sample-preparation plate for parallel processing of up to 96 protein samples prior to LC-MS/MS proteomics. The Micro format is designed for 1–100 µg of total protein per well and uses S-Trap™ technology for SDS-based protein solubilization, protein trapping, contaminant removal, and enzymatic digestion directly within the plate. 

The 96-well format is suitable for high-throughput and automated workflows. Plates can be processed by centrifugation, vacuum, or positive pressure and are compatible with automated platforms including the Tecan A200 Positive Pressure Workstation and Agilent Bravo.

S-Trap™ Technology

S-Trap™ sample processing enables the use of SDS for efficient biological sample lysis and protein solubilization. Proteins are subsequently acidified to pH <1 and exposed to a high concentration of methanol, producing extensive denaturation and eliminating unwanted enzymatic activity such as proteases and phosphatases. 

Denatured, undigested proteins are trapped within the pores of the derivatized S-Trap™ matrix through multiple weak-affinity interactions. Contaminants are removed during washing, after which the selected protease is added directly to the plate. The resulting peptides are not retained by the S-Trap™ matrix and can be eluted for downstream LC-MS/MS. 

Standard Workflow

1. Protein solubilization and denaturation in SDS
2. Reduction
3. Alkylation
4. Acidification to pH ≤1
5. Addition of methanolic Binding/Wash Solution
6. Sample transfer to the S-Trap™ 96-well Micro Plate
7. Protein trapping
8. Washing and contaminant removal
9. Protease addition
10. In-plate digestion
11. Peptide elution
12. Concentration and preparation for LC-MS/MS 

Technical Specifications

• Format: 96-well plate
• Samples per Plate: up to 96
• Total Protein Amount: 1–100 µg per well
• Maximum Stated Protein Load: ≤100 µg per well
• Approximate Well Capacity: 400 µL
• Standard Centrifugation: 2,000 × g for 2 min
• Alternative Flow Methods: vacuum or positive pressure
• Standard Washing: 250 µL Binding/Wash Solution, 3 times
• Protease Solution Volume: 20 µL
• Protocol Protease-to-Protein Ratio: 1:10 (w/w)
• Digestion: total of 2 h at 47 °C
• First Elution: 40 µL Digestion Solution
• Second Elution: 40 µL Elution Solution
• Optional Third Elution: 40 µL 50% ACN / 0.2% formic acid for hydrophobic peptides
• Downstream Analysis: LC-MS / LC-MS/MS
• Automation: compatible with selected 96-well automation systems
• Intended Use: Research Use Only – not for diagnostic procedures 

Available Pack Sizes

• PN P002-96MCR-0001PL – 1 S-Trap™ 96-well Micro Plate
• PN P002-96MCR-0003PL – 3 S-Trap™ 96-well Micro Plates
• PN P002-96MCR-0010PL – 10 S-Trap™ 96-well Micro Plates

Optional Accessory Pack

The S-Trap™ 96-well Micro Plate with Accessory Pack, PN P002-96MCR-0001AP, includes:

• 1 S-Trap™ 96-well Micro Plate
• 1 × 2.0 mL 96-well waste plate
• 1 × 1.0 mL 96-well collection plate
• 1 silicone mat. 

Standalone S-Trap™ 96-well Micro Plates do not include workflow solutions. The separate S-Trap™ 96-well Micro Kit provides the plate, accessories, and premade solutions for the complete workflow.

Typical Applications

• High-throughput bottom-up proteomics
• Shotgun proteomics
• Automated proteomics sample preparation
• LC-MS/MS sample preparation
• SDS-compatible proteomic workflows
• Cell and tissue lysates
• Membrane and poorly soluble proteins
• Complex biological samples
• Label-free proteomics
• TMT and other isobaric-labeling workflows
• Automated multiwell sample preparation