The TB4/TB-500/TB4(17-23) Analysis is a targeted laboratory test designed to identify and quantify TB4, TB-500, and the TB4(17-23) fragment and to assess peptide purity in lyophilised vials and raw active pharmaceutical ingredient (API) powders.
The analysis determines whether the sample contains the complete 43-amino-acid TB4 peptide or only a fragment. It can also specifically identify the TB4(17-23) fragment. Results for lyophilised samples are reported in milligrams per vial together with peptide purity.
About TB4, TB-500 and TB4(17-23)
Thymosin beta-4 (TB4) is a naturally occurring 43-amino-acid peptide involved in actin regulation, cell migration, and processes associated with tissue repair. TB-500 refers to synthetic forms related to TB4, while TB4(17-23) is a short fragment with the sequence LKKTETQ, studied mainly in relation to cell migration, angiogenesis, and wound healing.
Key Features
- Targeted identification and quantification of TB4, TB-500, and TB4(17-23)
- Distinguishes the complete 43-amino-acid TB4 peptide from shorter fragments
- Specific identification of the TB4(17-23) fragment
- Determination of peptide purity
- Suitable for lyophilised vials and raw API powders
- Analysis by HPLC
- Results for lyophilised samples reported in mg per vial and peptide purity
- Alternative result format for raw API powders includes purity in % (m/m) and peptide purity
- Minimum sample amount of one whole unopened vial with lyophilisate
- Minimum sample amount of 30 mg for raw API powders
- Average turnaround time of 7 business days
Analysis Parameters
- Target compounds: TB4, TB-500, TB4(17-23)
- Other names: Thymosin Beta-4, Thymosin Beta 4 Fragment, TB4 Fragment 17-23
- Testing method: HPLC
- CAS number – TB-500 (TB4): 77591-33-4
- Molecular weight – TB-500 (TB4): 4963 Da
- Sequence – TB-500 (TB4): Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Gln-Glu-Gly-Lys-Glu-Asn-Glu-Ser-OH
- CAS number – TB500 (889 Da): 476014-70-7
- Molecular weight – TB500 (889 Da): 889 Da
- Sequence – TB500 (889 Da): N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln
- Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial
- Result format for lyophilised samples: mg per vial and peptide purity
- Result format for raw API powders: Purity in % (m/m) and peptide purity
- Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate
- Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture
- Average turnaround time: 7 business days
Important Notice
- The analysis determines whether the complete 43-amino-acid TB4 peptide or only a fragment is present and then determines its amount and peptide purity.
- Results are distinguished in the laboratory report as follows:
- TB-500 (TB-4): the entire peptide was identified.
- TB-500: only a fragment was identified; the report comment specifies which fragment was detected.
- TB-500 889 (Da): the 17-23 fragment was identified.
- This analysis does not automatically screen the sample for other peptides, degradation products, contaminants, or undeclared substances.
- For lyophilised samples, one whole unopened vial containing lyophilisate is required.
- Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.
Optional Additional Analyses
The following optional laboratory services are available:
- TFA Analysis
- Complete Raw Analytical Data
- Fentanyl Screening
- Alternative Result Format: Purity in % (m/m)
- Batch Variance Testing
- pH Measurement
- Endotoxin Analysis
- CHNS Mass Report
- LCMS Screening for Peptide Contamination
- Sterility Testing (TAMC + TYMC)
- Heavy Metals Analysis (As, Cd, Pb, Hg)
- Additional Laboratory Report
