The omniDAWN™ MALS Detector is a next-generation multi-angle light scattering system specifically designed for UPLC™ and UHPLC workflows. It delivers absolute molecular weight and particle size measurements without relying on column calibration or molecular standards, allowing researchers to obtain highly accurate characterization data at true high-performance chromatography speeds.
Equipped with 18 optimally positioned scattering angles and a flow cell engineered to minimize stray light, omniDAWN measures molar masses from 200 Da to 1 GDa and particle radii from 10 nm to 1 µm. The system is ideally suited for characterization of proteins, antibody-drug conjugates (ADCs), lipid nanoparticles (LNPs), viral vectors, polymers, and other complex macromolecular systems.
Combined with ASTRA™ software, omniDAWN provides comprehensive characterization of molecular size, molar mass, composition, and stoichiometry. Integrated health monitoring and the COMET™ ultrasonic cleaning module ensure reliable operation, minimal maintenance, and consistent analytical performance.
Key Features
- Purpose-built for UPLC™ and UHPLC systems
- Absolute molecular weight determination without calibration standards
- Absolute particle size characterization
- 18 optimized light scattering angles
- Molecular weight range from 200 Da to 1 GDa
- Particle radius range from 10 nm to 1 µm
- High sensitivity with minimal stray light interference
- Up to 4× faster LC-MALS analysis using UPLC
- Reduced sample consumption
- Improved chromatographic resolution
- Advanced workflows for ADCs, LNPs, viral vectors, and polymers
- ASTRA™ software for advanced data analysis
- Integrated System Ready Monitor
- COMET™ ultrasonic in situ cleaning
- Real-time health diagnostics
- Automated system performance monitoring
Technical Specifications
Technology
- Multi-Angle Light Scattering (MALS)
- 18 light scattering detectors
- Low stray-light flow cell design
Measurement Range
- Molecular weight: 200 Da to 1 GDa
- Particle radius: 10 nm to 1 µm
Performance
- UPLC™ and UHPLC compatible
- Up to 4× faster than conventional HPLC-MALS
- Improved chromatographic resolution
- Reduced sample requirements
Waters/Wyatt: A) Achieve up to 4× faster LC-MALS runs with UPLC. B) Achieve superior separation, revealing fragment peaks not discernible by HPLC.
Software
- ASTRA™ Software
- Molecular weight analysis
- Particle size determination
- Conjugate stoichiometry analysis
- ADC characterization
- LNP characterization
- Viral vector characterization
- Polymer branching analysis
Waters/Wyatt: ASTRA software goes beyond measuring overall molecular weight by: A) uniquely resolving individual components to quantify protein and conjugated species and determine stoichiometry, and B) reporting the total protein fraction.
Monitoring and Maintenance
- System Ready Monitor
- Real-Time Health Monitoring
- COMET™ ultrasonic cleaning
- Automated diagnostics
- Continuous system performance verification
Typical Applications
- Monoclonal antibodies
- Antibody-drug conjugates (ADCs)
- Lipid nanoparticles (LNPs)
- Viral vectors
- Proteins and peptides
- Polymer materials
- Conjugated polymers
- Biotherapeutics
- Nanotechnology research
