The KNAUER Sepapure® oliGO 120-3 is a high-performance HPLC/UHPLC column optimized for oligonucleotide analysis in both reversed-phase (RP) and ion-pair reversed-phase (IP-RP) modes.
The column is based on hybrid silica with advanced endcapping and hydrophobic modification, ensuring stable interactions, high reproducibility, and long lifetime, even under demanding conditions.
With 3 µm particles, the column delivers UHPLC performance while maintaining low back pressure, allowing use even on standard HPLC systems.
It is ideal for:
- identification
- quantification
- quality control (QC)
of oligonucleotides.
KNAUER: Sepapure oliGO 120-3 Column
Key Features
- Optimized for oligonucleotides
- Supports RP and IP-RP modes
- UHPLC performance with low back pressure
- 3 µm particles for high resolution
- 18% carbon load
- Wide pH range (1.5–10)
- High chemical stability
- Compatible with standard HPLC systems
- High reproducibility and robustness
- Suitable for extreme pH and temperature conditions
Technical Specifications
General
- Separation modes: RP / IP-RP
- Endcapping: Yes
- USP classification: L1
- Connection: 1/16"
Column Parameters
- Particle size: 3 µm
- Pore size: 120 Å
- Surface area: 300 m²/g
- Carbon load: 18%
Operating Conditions
- pH range: 1.5 – 10
- Max pressure: 413 bar
- Max temperature: 60 °C
Technology Advantages
Low Back Pressure
- enables UHPLC performance on HPLC systems
- protects instrumentation
- reduces wear and clogging
- minimizes oligonucleotide aggregation
IP-RP Optimization
- ideal pH range: 7.0 – 9.5
- combines hydrophobic and ionic interactions
- enables high-resolution separation
Cost-effective Hardware
- no need for inert hardware
- optimized for routine analytical use
- tested with no impact on performance
Typical Applications
- Oligonucleotide analysis
- Biopharmaceutical QC
- Polar molecule analysis
- Method development (extreme pH/temperature)
