Primarily supports legacy methods. Symmetry columns give you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.
With the Symmetry C18 Column, you will gain excellent reproducibility year after year. With each batch of new columns, there is no need to revalidate and redevelop techniques. The lab equipment enables the labor costs associated with standard analytical columns that require QC column testing to be reduced.
The Symmetry C18 Columns are manufactured in cGMP, ISO9001, and ISO13485 certified facilities, ensuring that each step is monitored and the toughest criteria are maintained. They offer the tightest specifications in the market, ensuring consistent results every time. This is accomplished by designing Symmetry C18 Columns with high purity raw materials and column packaging techniques that provide the most repeatable HPLC column.
With the monofunctionally bound C18 ligand included in these columns, you can increase efficiency and precision in your lab. For optimum reproducibility, they additionally comprise a high purity base-deactivated silica. Symmetry C18 Columns also offer the industry's tightest ligand surface coverage (ligand density) standards, resulting in minimum resolution changes and more consistent batch-to-batch results.
Waters: Symmetry C18 HPLC Columns
Specifications
- Chemistry: C18
- Separation Mode: Reversed Phase
- Particle Substrate: Silica
- pH Range Min: 2 pH
- pH Range Max: 8 pH
- Maximum Pressure: 6000 psi (415 Bar)
- Endcapped: Yes
- Silanol Activity: Low
- Particle Shape: Spherical
- Pore Size: 300 Å
- System: HPLC
- USP Classification: L1
- Carbon Load: 8.5 %
The Waters Symmetry C18 HPLC Column is also available in these types:
Waters Symmetry C18 HPLC Column with eConnect Column Tag
- This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.
Waters: Symmetry C18 HPLC Column with eConnect Column Tag
Waters Symmetry Shield RP18 HPLC Column
- These reversed-phase columns contain Waters patented embedded polar group technology that “shields” the silica’ residual silanol surface from highly basic analytes. The reduced silanol activity for the SymmetryShield RP18 column significantly improves peak shape and resolution.
- Bonding Technology: Shield RP18
- Pore Size: 100 Å
- Carbon Load: 17 %
Waters Symmetry Shield RP18 HPLC Column with eConnect Column Tag
- This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.
Symmetry HPLC Columns
Silica-based columns that exceed HPLC column performance expectations
Reproducible HPLC columns are essential when developing analytical methods for pharmaceutical and biopharmaceutical products. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.
Available in column and guard formats, Symmetry Columns are manufactured using high purity silica and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.
Overview
- Deliver maximum reproducibility batch-to-batch and column-to-column
- Provide superior peak shape
- Achieve high recoveries of peptides and proteins
- Manufactured using high purity silica and tightly controlled manufacturing processes
- Recommended Use: For rugged and reproducible HPLC column performance.
Waters: Symmetry Columns
Rugged and reproducible HPLC column performance
With Symmetry HPLC Columns, you’ll achieve reliable column-to-column and batch-to-batch reproducibility. Symmetry’s narrow column specification ranges minimize variation, giving you the confidence that the methods you use today will continue to be used in the future.
Waters: Symmetry HPLC Columm - Unmatched Year to year Reproducibility
Superior peak shape
Symmetry Shield Columns feature the Waters patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes and improves overall peak shape. Placing the embedded polar group close to the silica surface further reduces surface silanol activity, and the embedded polar group technology allows you to improve chromatographic peak shape and selectivity in the most challenging of method conditions.
Waters: Symmetry HPLC Column - Embedded Polar Group Technology improves chromatographic peak shape and selectivity
Deliver high peptide recovery
All Symmetry300 columns are QC tested with peptide samples to ensure exceptional batch-to-batch reproducibility. These wide-pore (300 Å), reversed-phase chromatography columns deliver superior performance when simultaneously separating hydrophilic and hydrophobic peptides, neutral, and highly basic peptides. With a Symmetry300 column, you can expect better performance with either high or low mass loads.
Waters: Symmetry HPLC Column - Compared to many competitive 100% silica-based C18 columns, Waters proprietary bonding and end-capping technologies
