Phenyl columns for Reversed Phase in HPLC and UHPLC (USP L11)
Phenyl stationary phases (USP L11) in reversed phase mode with alternative selectivity for:
- Aromatic and moderately π-π interaction polar analytes
- Mixtures with varying polarity and aromaticity
The polarity is similar to C8 modification but with different selectivity for polar aromatics, polycyclic aromatic hydrocarbons, fatty acids etc. Phenyl phases with a classical slightly hydrophobic endcapping or without endcapping.
Properties
- Separation mechanism: π-π interactions
- Ultra pure, spherical high performance HPLC phase based on silica gel
- Phenyl modification (Phenylpropyl), 12 % carbon content
Key features
- Classical phenyl phase with 12 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications.
Recommended application areas
- Alternative selectivity for aromatic and mode rately pi-pi interaction polar analytes or mixtures with varying polarity and aromaticity.
KNAUER: Phenyl
Tip: For UHPLC columns like Eurospher II 2 μm particle size, samples and eluents should be filtered through a 0.2 μm instead of a 0.45 μm membrane filter. Frits at column inand outlet have smaller pores than classical HPLC columns and block more easily.
Stationary phase Eurospher II 100 Phenyl is provided in these column types: analytical, semi-preparative and preparative.
Eurospher II 100 Phenyl Preparative Standard Columns are available with these parameters:
- Column length (mm): 50, 150, 250
- Column ID (mm): 20, 30, 50
- Particle size (µm): 5, 10, 15
Eurospher II 100 Phenyl Preparative dAX Columns with dynamic axial compression are available with these parameters:
- Column length (mm): 50, 150, 250
- Column ID (mm): 25, 30, 50
- Particle size (µm): 5, 10, 15
KNAUER Preparative HPLC Columns
The right choice for all dimensions from semi-prep to batch chromatography to SMB.
- Modifications: Reversed Phase, HILIC, Normale Phase
- Max. Pressure: 400–600 bar
- Column Inner Diameter (ID): 16–50 mm in our shop, more on request
- Column Brands: Eurospher, Eurospher II
- Safe Investment: Robustness and durability based on superior technology
- Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
- Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available
KNAUER: KNAUER Preparative HPLC Columns
Preparative HPLC columns can be offered with two different hardware types:
- Versatile standard flanged hardware for nearly any application task in batch chromatography
- dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes
