Classical Reversed Phase columns for HPLC and UHPLC (USP L1)
Most often used stationary phase in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.
Properties
- Separation mechanism: Hydrophobic interaction
- Ultra pure, spherical high performance HPLC phase based on silica gel
- Unpolar, monomeric C18 (Octadecyl) modification, endcapped, with 16 % carbon content (~ 50 % endcapping).
Key features
- High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical C18 phase with ca. 50 % endcapping and resulting 16 % carbon content, outstanding mechanical and chemical stability.
Recommended application areas
- acidic, basic and neutral analytes in reversed phase mode, for example sulphonamides
- anabolic steroids
- anti-psychotics
- beta blocker
- sudan dyes
- phenols and preservatives
KNAUER: C18 Non-endcapped
Tip: Caution! Never use classical C18 phases with 100 % aqueous mobile phase. The hydrophobic C18 chains will collapse
Stationary phase Eurospher II 100 C18 is provided in these column types: analytical, semi-preparative and preparative.
Eurospher II 100 C18 Preparative Standard Columns are available with these parameters:
- Column length (mm): 50, 150, 250
- Column ID (mm): 20, 30, 50
- Particle size (µm): 5, 10, 15
Eurospher II 100 C18 Preparative dAX Columns with dynamic axial compression are available with these parameters:
- Column length (mm): 50, 150, 250
- Column ID (mm): 25, 30, 50
- Particle size (µm): 5, 10, 15
KNAUER Preparative HPLC Columns
The right choice for all dimensions from semi-prep to batch chromatography to SMB.
- Modifications: Reversed Phase, HILIC, Normale Phase
- Max. Pressure: 400–600 bar
- Column Inner Diameter (ID): 16–50 mm in our shop, more on request
- Column Brands: Eurospher, Eurospher II
- Safe Investment: Robustness and durability based on superior technology
- Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.
- Customizable: Standard flanged hardware & dAX hardware with static or dynamic axial compression available
KNAUER: KNAUER Preparative HPLC Columns
Preparative HPLC columns can be offered with two different hardware types:
- Versatile standard flanged hardware for nearly any application task in batch chromatography
- dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes
