Classical Reversed Phase columns for HPLC and UHPLC (USP L1)
Most often used stationary phase in reversed phase mode. Silica gel based columns with C18 modification are available with or without endcapping. Classically endcapped phases are slightly more hydrophobic and unpolar than the not endcapped phases.
Properties
- Separation mechanism: Hydrophobic interaction
- Ultra pure, spherical high performance HPLC phase based on silica gel
- Unpolar, monomeric C18 (Octadecyl) modification, endcapped, with 16 % carbon content (~ 50 % endcapping).
Key features
- High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical C18 phase with ca. 50 % endcapping and resulting 16 % carbon content, outstanding mechanical and chemical stability.
Recommended application areas
- acidic, basic and neutral analytes in reversed phase mode, for example sulphonamides
- anabolic steroids
- anti-psychotics
- beta blocker
- sudan dyes
- phenols and preservatives
KNAUER: C18 Non-endcapped
Tip: Caution! Never use classical C18 phases with 100 % aqueous mobile phase. The hydrophobic C18 chains will collapse
Stationary phase Eurospher II 100 C18 is provided in these column types: analytical, semi-preparative and preparative.
Eurospher II 100 C18 Analytical Columns are available with these parameters:
- Column length (mm): 50, 100, 150, 250
- Column ID (mm): 2, 3, 4, 4.6
- Particle size (µm): 2, 3, 5
Eurospher II 100 C18 Semi-Preparative Standard Columns are available with these parameters:
- Column length (mm): 30, 50, 125, 150, 250
- Column ID (mm): 8, 16
- Particle size (µm): 5, 10
KNAUER (U)HPLC Columns
Widest range of HPLC and UHPLC columns for all separation modes and applications.
- Safe Investment: Robust and reliable columns for attractive price
- Separation Mode: Reversed Phase, HILIC, Normale
- Simplicity: Easy purchase via our online store with fast delivery
- Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
- Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar
KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.
KNAUER: KNAUER (U)HPLC Columns
