A Platform Method for the Molecular Mass Analysis of the Light Chains and Heavy Chains of Monoclonal Antibodies using the BioAccord System

Analysis of Monoclonal Antibodies and Their Fragments by Size Exclusion Chromatography Coupled with an Orbitrap Mass Spectrometer Shanhua Lin,1 Hongxia Wang, 2 Zhiqi Hao, 2 Patrick Bennett, 2 and Xiaodong Liu1 1 Thermo Fisher Scientific, Sunnyvale, CA, USA; 2Thermo Fisher…

HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS MONITORING WORKFLOW Samantha Ippoliti1, Bradley Prater2, Ying Qing Yu1, Magnus Wetterhall3, Guillaume Bechade4 1 Waters Corporation, Milford, MA USA; 2Similis Bio, Sunnyvale, CA; 3Waters Corporation, Solna, Sweden; 4Waters Corporation, Wilmslow,…

Agilent-NISTmAb Intact & Subunit Analysis Using Reversed Phase Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…

An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements Application Note Biotherapeutics and Biosimilars Authors Introduction Shuai Wu and Maryann Shen Monoclonal antibody (mAb) based entities represent a rapidly growing class of biologics that require extensive…