High-throughput peptide mapping of trastuzumab using a tandem LC-MS workflow

APPLICATION NOTE 21835 An automated high-throughput workflow for peptide mapping to monitor post-translational modifications (PTMs) of monoclonal antibodies Authors Silvia Millán-Martín, Craig Jakes, Giorgio Oliviero, Sara Carillo, Jonathan Bones Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing…

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…

APPLICATION NOTE 21782 Comparison of alternative approaches to trypsin protein digestion for reproducible and efficient peptide mapping analysis of monoclonal antibodies Authors Silvia Millán-Martín, Craig Jakes, Noemi Dorival-García, Nicola McGillicuddy, Sara Carillo, Amy Farrell, Jonathan Bones National Institute for Bioprocessing…

APPLICATION NOTE 73672 Accelerating monoclonal antibody peptide mapping with high acquisition speed Orbitrap-based MS Authors: Phil Widdowson, Amy Claydon, Tom Buchanan Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modification, PTM, high throughput analysis, golimumab,…