Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS

A novel approach for multiple PQAs monitoring of mAbs from bioreactors using 2D-LC-MS Maria Grübner a, Christof Mitterer b, Alexander Schwahn c, Aleš Holfeld c, Sara Carillo e, Ken Cook d, Jonathan Bones e a-d Thermo Fisher Scientific (a Germering/Germany;…

Application note | 004276 Biopharma Monitoring monoclonal antibody bioproduction processes with 2D-LC-UV Author Application benefits Maria Grübner, Thermo Fisher Scientific, • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of cell culture samples enables titer determination and purification for subsequent…

Process Analytics A switchable 2D-LC platform in PAT: routine UV monitoring or MS-based characterization of mAb PQAs directly from the bioreactor Maria Grübnera, Runsheng Zhenga, Alexander Schwahnb, Ken Cookc, Thermo Fisher Scientific, a Germering (Germany), b Reinach (Switzerland), c Hemel…

APPLICATION NOTE 21880 Comparing biosimilars using intact mass analysis under denaturing and native conditions Authors Sara Carillo, Izabela Zaborowska, Jonathan Bones National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland Keywords NIBRT, biopharmaceutical, biotherapeutic, monoclonal antibody (mAb), IgG, biosimilar,…