Capsid Protein Analysis of Viral Vector- Based Gene Therapeutics by Hydrophilic Interaction Chromatography

[ APPLICATION NOTE ] Optimizing Adeno-Associated Virus (AAV) Capsid Protein Analysis Using UPLC and UPLC-MS Ximo Zhang, Stephan Koza, Ying Qing Yu, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ INTRODUCTION An optimized LC-MS solution for…

Application note | 001120 Biopharma Assessing key attributes of adeno-associated viral proteins using HPLC-FLD-intact mass analysis Authors Application benefits Reiko Kiyonami1, Roberto Gamez1, Min • Rapid ratio assessment of adeno-associated virus (AAV) structural proteins VP1:VP2:VP3 through sensitive fluorescence detection •…

Assessing key attributes of adeno-associated viral proteins using UHPLC-FLD-intact accurate mass analysis Reiko Kiyonami1, Roberto Gamez1, Min Du2, Kenneth Thompson3, Chao Yan Liu3, Julia Kraegenbring4, Catharina Crone4 1Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Cambridge, MA, USA;…

Development of an online two-dimensional SEC-UV-RPLC-MS method for the multi-attribute characterization of adeno-associated virus products MULTIDIMENSIONAL CHROMATOGRAPHY WORKSHOP 4th February 2025 Megane Aebischer [email protected] Introduction Genetic disease Defective DNA Defective protein Introduction 2D-LC workflow Applications Conclusion 2 [email protected] Adeno-associated virus…