Green ion pair and HFIP free method for ASO RNA analysis with GLP compliant automated data handling

Oligonucleotide analysis Detailed study into ASO impurity analysis, lessons learned, and myths dispelled while moving to compliant platform methods Ulrik H. Mistarz1, Marcus Hoffmann2, Heiko Herrmann2, Richard Law3, Alexander Schwahn4, Ken Cook5 1Thermo Fisher Scientific, Denmark, 2Thermo Fisher Scientific, Germany,…

Method considerations for therapeutic ASO RNA analysis. Adducts and insource impurity generation Ken Cook1, Ulrik Mistarz1, Alexander Schwahn2, Fiona Rupprecht3 1 Thermo Fisher Scientific, Hemel Hempstead, United Kingdom, 2Thermo Fisher Scientific, Reinach, Switzerland, 3 Thermo Fisher Scientific, Dreieich, Germany Abstract…

Oligonucleotides Capillary flow ion pair reversed-phase separation for very sensitive oligonucleotide LC-HRMS analysis and characterisation Ulrik H. Mistarz1, Shanhua Lin2, Shane L. Bechler2, Brandon H. Robson2, Ken Cook3 1) Thermo Fisher Scientific, Allerød, Denmark, 2) Thermo Fisher Scientific, Sunnyvale, CA…

Cation exchange coupled to high resolution mass spectrometry for the analytical characterization of phosphorodiamidate morpholino oligomers K Cook[a];U H Mistarz[b]; S Millán-Martín[c]; S Carillo[c]; J Bones[c] [a]Thermo Fisher Scientific, United Kingdom; [b]Thermo Fisher Scientific, Denmark; [c]National Institute for Bioprocessing Research…