Using Novel RNases to Measure 5’ Cap and Poly(A) Tail Modifications during Oligonucleotide Mapping LC-MS of mRNA

Application Note Tunable Digestions of RNA Using RapiZyme™ RNases to Confirm Sequence and Map Modifications Balasubrahmanyam Addepalli, Tatiana Johnston, Christian Reidy, Matthew A. Lauber Waters Corporation Abstract RNA therapeutics such as sgRNA and mRNA are important modalities for Gene Therapy…

Application Note RNA Digestion Product Mapping Using an Integrated UPLC-MS and Informatics Workflow Catalin E. Doneanu, Chris Preston, Matt Gorton, Tatiana Johnson, Bala Addepalli, Ying Qing Yu Waters Corporation Abstract LC-MS analysis is an indispensable tool for single-guide RNA (sgRNA)…

sgRNA AND mRNA SEQUENCE MAPPING VIA ENDONUCLEASE DIGESTION AND LC-MS ANALYSIS, USING NOVEL INFORMATICS WORKFLOWS Catalin Doneanu1, Alexandre F. Gomes1, Chris Preston2, Matt Gorton2, Tatiana Johnson1, Bala Addepalli1, Heidi Gastall2 and Ying Qing Yu1 1Waters Corporation, Milford, MA, USA; 2Waters…

Application Note Sequence Mapping of mRNA Digests Using the Xevo™ MRT Mass Spectrometer and waters_connect™ MAP Sequence 2.0 Application Catalin Doneanu, Alexandre F. Gomes, Chris Preston, Matt Gorton, Tatiana Johnston, Bala Addepalli, Ying Qing Yu Waters Corporation, United States Published…