Screening of Sample Matrices and Individual Matrix Ingredients for Suitability in AAA-Direct™

Application Note 150 Determination of Amino Acids in Cell Cultures and Fermentation Broths INTRODUCTION Cell cultures and fermentation broths are used in the manufacture of biotherapeutics and many other biological materials produced using recombinant genetic technology. Broths are also used…

Application Note 163 Determination of Protein Concentrations Using AAA-Direct™ INTRODUCTION Dye-binding assays such as bicinchoninic acid (BCA), Lowry, and Bradford (Coomassie) are the most common methods to determine total protein. The BCA assay depends on Cu (II) reduction to Cu…

Application Note 130 Identification of a Hydroxylysine-Containing Peptide Using AAA-Direct™ INTRODUCTION One type of protein microheterogeneity is a result of the posttranslational modification of lysine (Lys) to hydroxylysine (Hyl). This modification normally occurs in collagen and a few other proteins…

Application Note 179 Carbohydrate and Amino Acid Analysis Using 3-D Amperometry INTRODUCTION AAA-Direct™ is an established technique for amino acid analysis. This technique has proven capabilities for separation of amino acids and carbohydrates in complex samples containing a large number…