Determination of Amino Acids in Cell Cultures and Fermentation Broths

Application Update 152 An Improved Gradient Method for the AAA-Direct™ Separation of Amino Acids and Carbohydrates in Complex Sample Matrices INTRODUCTION AAA-Direct is an established technique for amino acid analysis. This technique has proven capabilities for the separation of amino…

Application Note 179 Carbohydrate and Amino Acid Analysis Using 3-D Amperometry INTRODUCTION AAA-Direct™ is an established technique for amino acid analysis. This technique has proven capabilities for separation of amino acids and carbohydrates in complex samples containing a large number…

APPLICATION NOTE 122 The determination of carbohydrates, alcohols, and glycols in fermentation broths Authors Valoran Hanko and Jeffrey S. Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Keywords Dionex CarboPac MA1 column, Dionex CarboPac PA1 column, pulsed amperometric detection, PAD, high-performance…

Application Note 142 Determination of Tryptophan Using AAA-Direct ™ INTRODUCTION Tryptophan (Trp) is a difficult amino acid to determine in proteins and peptides because it chemically decomposes during acid hydrolysis. Consequently, many procedures have been developed for the independent preservation…