Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis

Deanna Hurum, Lipika Basumallick, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Prostate-Specific Antigen (PSA), Transferrin, N-Glycans, O-Glycans, Dionex CarboPac Column Introduction Changes in protein glycosylation are frequently studied in cancer research to identify potential biomarkers. Factors…

Application Note 215 Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column Introduction High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a widely used technique for determining an extensive set of carbohydrates including, but…

APPLICATION NOTE 72914 HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual eluent generation cartridges Authors Beibei Huang and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal Demonstrate Dual EGC capability and performance for profiling N-linked oligosaccharides released from…

Application Note 105 Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography INTRODUCTION Glycoforms and Protein Sialylation Glycoproteins are proteins with a carbohydrate attached to the polypeptide backbones through one or more glycosylation sites. Oligosaccharides can be linked…