Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD

APPLICATION UPDATE 176 Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis Authors Introduction Pranathi Perati and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Glycosylation is one of the most important post-translational modifications in eukaryotic cell proteins. Glycoproteins are involved…

APPLICATION NOTE 72914 HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual eluent generation cartridges Authors Beibei Huang and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal Demonstrate Dual EGC capability and performance for profiling N-linked oligosaccharides released from…

Application Note 105 Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography INTRODUCTION Glycoforms and Protein Sialylation Glycoproteins are proteins with a carbohydrate attached to the polypeptide backbones through one or more glycosylation sites. Oligosaccharides can be linked…

TECHNICAL NOTE 72264 HPAE-PAD N-linked oligosaccharide profiling of IgG Authors Sachin Patil, Deanna Hurum, Lipika Basumallick, and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Goal To develop an HPAE-PAD method to profile the major neutral and charged asparagine-linked (N-linked) oligosaccharides…