Analysis of Deaminated Oligonucleotides Using Supercritical Fluid Chromatography

Application Note No. 100 Short-Chain Oligonucleotide Analysis Using Supercritical Fluid Chromatography Momoka Hayashida Life Science Life Science  Abstract On the other hand, since the mobile phase containing carbon dioxide has low polarity, SFC was considered unsuitable for the analysis…

Application Note Platform Ion Pairing RPLC Method for Oligonucleotides Using High Throughput 20 mm Length Columns Szabolcs Fekete, Mateusz Imiolek, Matthew A. Lauber Waters Corporation Abstract A novel generic (platform) method is proposed for the separation of oligonucleotide size variants…

Application Note Biopharma/Pharma Evaluating HILIC Stationary Phases for Oligonucleotide Separation by LC/MS Authors Abstract Jordy Hsiao, Lee Bertram, Alex Apffel, Andrea Angelo P. Tripodi, Andrew Coffey, Ta-Chen Wei, and Connor Flannery Agilent Technologies, Inc. Hydrophilic interaction chromatography (HILIC) represents a…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…