Structural Analysis of Native N-Glycans Released from Proteins Using a Novel Mixed-Mode Column and a Hybrid Quadrupole-Orbitrap Mass Spectrometer

See your protein therapeutics in high resolution Bio LC Columns Contents 2 Introduction to monoclonal antibody (mAb) characterization 3 Affinity titer determination 4 Aggregate separation 5 Charge variant analysis 6 Oxidation variant analysis 11 Antibody drug conjugates (ADCs) analysis 12…

In M M G N-linked glycans are released from proteins by PNGase-F. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased…

O-linked glycans are released from proteins by reductive β-elimination. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased performance and ease…

Dave Thomas and Ian Acworth Thermo Fisher Scientific, Chelmsford, MA Ap plica t ion Note 1 1 27 Label-Free Analysis by UHPLC with Charged Aerosol Detection of Glycans Separated by Charge, Size, and Isomeric Structure Key Words Glycan Quantitation, Glycan…