Structural characterization of the therapeutic antibody and biosimilar product with hydrogen deuterium exchange mass spectrometry

TECHNICAL NOTE 65887 Hydrogen deuterium exchange mass spectrometry for protein structural characterization Authors: Terry Zhang and Rosa Viner Thermo Fisher Scientific, San Jose, CA, USA Keywords: Peptide mapping, protein conformation, bottom-up, deuterium uptake, hydrogen deuterium exchange mass spectrometry, HDX-MS, HDX,…

WHITE PAPER 65872 Hydrogen deuterium exchange mass spectrometry for the masses Executive summary Determining the shape and dynamics of proteins and protein complexes is crucial to understanding protein function and the roles proteins play in biological systems. Traditional techniques employed…

Analytical solutions for biopharmaceutical characterization and control Your partner on every step of your journey Together we can address the challenges of biotherapeutic drug development Your molecules are complex Biotherapeutics such as monoclonal antibodies, biosimilars, antibody-drug conjugates, and nucleotide-based therapies…

LOCALIZED CONFORMATION ANALYSES OF MUTATED HUMAN IgG1 BY HYDROGEN DEUTERIUM EXCHANGE MASS SPECTROMETRY AND DIFFERENTIAL SCANNING CALORIMETRY Joomi Ahn 1, Xiaojun Lu2, Colette Quinn3, St John Skilton1, Ying Qing Yu1, and Jihong Wang2 1 Waters Corporation, 2 MedImmune, 3TA Instruments…