Efficient Non-Reduced mAb Subunit LC-MS Analysis to Screen for Modifications of Unpaired Cysteines in Innovator and Biosimilars

HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS MONITORING WORKFLOW Samantha Ippoliti1, Bradley Prater2, Ying Qing Yu1, Magnus Wetterhall3, Guillaume Bechade4 1 Waters Corporation, Milford, MA USA; 2Similis Bio, Sunnyvale, CA; 3Waters Corporation, Solna, Sweden; 4Waters Corporation, Wilmslow,…

HIGH THROUGHPUT CYSTEINYLATION SCREENING AT MAB SUBUNIT LEVEL USING LC-MS SCREENING WORKFLOW Samantha Ippoliti1, Bradley Prater2, Ying Qing Yu1, Magnus Wetterhall3 1Waters Corporation, Milford, MA; 2Similis Bio, Sunnyvale, CA, USA; 3Waters Corporation, Solna, Sweden INTRODUCTION RESULTS & DISCUSSION Monoclonal antibodies…

Application Note Similis Bio - Streamlined mAb Subunit LCMS Workflow for Multiple Attribute Monitoring of Biosimilar mAb Candidates During Bioprocessing and Development Samantha Ippoliti, Jared Young, Katy McNally, Caitlin Hanna, Ying Qing Yu, Bradley Prater, Mark D. Wrona, Emma Harry…

[ APPLICATION NOTEBOOK ] Multi-Attribute Methods for Biopharmaceutical Analysis Application Notes [ APPLICATION NOTEBOOK ] Introduction The adoption of LC-MS-based multi-attribute method (MAM) analysis for routine monitoring of biotherapeutic variation has progressed greatly over the last five years. The ability…