Evaluating the Ruggedness of the Alliance™ iS Bio HPLC System for SEC Separations

ADVANCING ANALYTICAL PERFORMANCE FOR BIOTHERAPEUTICS WITH A BIO-INERT HPLC SYSTEM Pawel Bigos, Xiangsha Du, Duanduan Han, Robert Birdsall, Karen Nyholm Waters Corporation INTRODUCTION RESULTS AND DISCUSSION In biopharmaceutical analysis, undesired secondary analyte/ surface interactions have hindered performance in HPLC. Analytes…

Application Note Waters ACQUITY and XBridge Premier Protein SEC 250 Å Columns: A New Benchmark in Inert SEC Column Design Lavelay Kizekai, Matthew A. Lauber Waters Corporation Abstract One of the major limitations in protein size-exclusion chromatography (SEC) is the…

Application Note Evaluation of Modern and Legacy HPLC Systems for the Size-Exclusion Analysis of Aggregation and Fragmentation in a Monoclonal Antibody Drug Corey E. Reed, Paula Hong Waters Corporation Abstract Size-exclusion chromatography is a widely used characterization technique for the…

[ CARE AND USE MANUAL ] ACQUITY Premier Protein SEC 250 Å, 1.7 µm Columns CONTENTS I. INTRODUCTION Thank you for choosing a Waters™ SEC Column. The ACQUITY™ I. INTRODUCTION II. SEC ANALYSIS OF PROTEIN AGGREGATES, MONOMERS, AND FRAGMENTS…