Fusion protein complexes analyzed by CG-MALS—non-equivalent, multivalent interactions

Light Scattering for the Masses ® Binding Affinity and Stoichiometry of a Multivalent Protein-aptamer Association Summary ecause proteins are often multimeric in their native state, the biomarker targets of many pharmaceuticals present multiple binding sites. In the case of monoclonal…

A P P L I C AT I O N N O T E AN3008: Metacomplex Formation & Binding Affinity of Multivalent Binding Partners Sophia Kenrick, Ph.D. and Dan Some, Ph.D., Wyatt Technology Summary Multivalent binding plays a crucial role…

Light Scattering for the Masses ® Measuring the Interaction Between Thrombin-α and an Anti-Thrombin Antibody Summary Antibody-antigen binding, hormone-receptor interactions, and many other common biomolecular interactions occur at stoichiometries other than 1:1. The Wyatt Calypso system utilizes composition-gradient multi-angle light…

W H I T E PA P E R WP3001: SEC-MALS and CG-MALS: Complementary Techniques to Characterize Protein-DNA Complexes Sophia Kenrick, Ph.D., Waters | Wyatt Technology Summary Interactions between proteins and nucleic acids often result in binding stoichiometries greater than…