Evaluation of the Temperature Influence on Retention for HILIC of Glucose Oligomers Labeled with 2-Aminobenzamide: Retention Thermodynamics and Practical Influence on Separation of Glycans

Mauro De Pra,1 Barbara van Cann,1 and Peter Sauter2 1 Thermo Fisher Scientific, Amsterdam, The Netherlands; 2Thermo Fisher Scientific, Germering, Germany Key Words Chromeleon 7.2 CDS, HILIC, Biomolecules, Dextran, UltiMate 3000 BioRS, Accucore Introduction Analysis of glycans labeled with 2-aminobenzamide…

APPLICATION NOTE 72604 Comparison of therapeutic antibody originator and biosimilar glycosylation using an integrated glycan labeling solution Authors Alexander Ley and Mauro De Pra Thermo Fisher Scientific, Germering, Germany Goal Demonstrate the suitability of the Applied Biosystems™ GlycanAssure™ HyPerformance APTS…

Application Note Biopharma Response (LU) Response (LU) Response (LU) Resolution and Speed in the Separation of Glycans 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 2.25 2.00 1.75 1.50 1.25 1.00 0.75 0.50 0.25 0.00 2.25 2.00 1.75…

Monica Dolci, Thermo Fisher Scientific, Runcorn, Cheshire, UK Te ch ni cal N ote 2 0 5 4 4 Hydrophilic Interaction Liquid Chromatography: Some Aspects of Solvent and Column Selectivity Key Words Hydrophilic, HILIC, Porous Graphitic Carbon (PGC), Selectivity, Zwitterionic…