Determination of Tryptophan Using AAA-Direct

Technical Note 50 Determination of the Amino Acid Content of Peptides by AAA-Direct INTRODUCTION EQUIPMENT ™ The AAA-Direct system separates amino acids on a high performance anion-exchange column and directly detects them by integrated amperometry.1, 2 AAA-Direct has advantages over…

Application Note 163 Determination of Protein Concentrations Using AAA-Direct™ INTRODUCTION Dye-binding assays such as bicinchoninic acid (BCA), Lowry, and Bradford (Coomassie) are the most common methods to determine total protein. The BCA assay depends on Cu (II) reduction to Cu…

Application Note 130 Identification of a Hydroxylysine-Containing Peptide Using AAA-Direct™ INTRODUCTION One type of protein microheterogeneity is a result of the posttranslational modification of lysine (Lys) to hydroxylysine (Hyl). This modification normally occurs in collagen and a few other proteins…

Application Note 150 Determination of Amino Acids in Cell Cultures and Fermentation Broths INTRODUCTION Cell cultures and fermentation broths are used in the manufacture of biotherapeutics and many other biological materials produced using recombinant genetic technology. Broths are also used…