Determination of the Amino Acid Content of Peptidesby AAA-Direct

Application Note 130 Identification of a Hydroxylysine-Containing Peptide Using AAA-Direct™ INTRODUCTION One type of protein microheterogeneity is a result of the posttranslational modification of lysine (Lys) to hydroxylysine (Hyl). This modification normally occurs in collagen and a few other proteins…

[ APPLICATION NOTEBOOK ] Amino Acid Analysis Application Notebook [ CONTENTS ] [ INTRODUCTION ] Waters UPLC Amino Acid Analysis Solution................................................................................9 UPLC Amino Acid Analysis Solution............................................................................................... 11 Enhancement of the UPLC Amino Acid Analysis Solution with Flexible Detector Options......................................................................................................... 15…

Application Note 142 Determination of Tryptophan Using AAA-Direct ™ INTRODUCTION Tryptophan (Trp) is a difficult amino acid to determine in proteins and peptides because it chemically decomposes during acid hydrolysis. Consequently, many procedures have been developed for the independent preservation…

Application Note 163 Determination of Protein Concentrations Using AAA-Direct™ INTRODUCTION Dye-binding assays such as bicinchoninic acid (BCA), Lowry, and Bradford (Coomassie) are the most common methods to determine total protein. The BCA assay depends on Cu (II) reduction to Cu…